CAJ | 학술논문

目的检测几种常见嗜肝DNA病毒在胆道闭锁患儿肝脏组织中的感染率,探讨其与胆道闭锁发生的关系.方法用荧光定量PCR(FQ-PCR)检测随机选取的85例胆道闭锁和10例对照组患儿肝脏组织中的5种嗜肝DNA病毒,包括人巨细胞病毒(HCMV)、腺病毒(ADV)、EB病毒(EBV)、单纯疱疹病毒(HSV)和乙肝病毒(HBV),同时进行石蜡切片的病理染色和巨细胞病毒晚期蛋白PP65免疫组化方法检测.结果在85例胆道闭锁患儿肝脏组织中,嗜肝DNA病毒阳性56例(65.9%),其中HCMV阳性51例(60.0%),ADV阳性5例(5.9%),EBV阳性3例(3.5%),HSV和HBV阳性0例(0%),对照组10例中均未检测到以上病毒的存在.免疫组织化学结果显示:HCMV多集中于肝脏组织中的肝细胞、血管内皮细胞、炎症浸润细胞当中,胆管上皮细胞呈强阳性反应.结论胆道闭锁与嗜肝DNA病毒感染之间存在相关性,其中以人巨细胞病毒感染关系最为密切.
목적 검측궤충상견기간DNA병독재담도폐쇄환인간장조직중적감염솔,탐토기여담도폐쇄발생적관계.방법 용형광정량PCR(FQ-PCR)검측수궤선취적85례담도폐쇄화10례대조조환인간장조직중적5충기간DNA병독,포괄인거세포병독(HCMV)、선병독(ADV)、EB병독(EBV)、단순포진병독(HSV)화을간병독(HBV),동시진행석사절편적병리염색화거세포병독만기단백PP65면역조화방법검측.결과 재85례담도폐쇄환인간장조직중,기간DNA병독양성56례(65.9%),기중HCMV양성51례(60.0%),ADV양성5례(5.9%),EBV양성3례(3.5%),HSV화HBV양성0례(0%),대조조10례중균미검측도이상병독적존재.면역조직화학결과현시:HCMV다집중우간장조직중적간세포、혈관내피세포、염증침윤세포당중,담관상피세포정강양성반응.결론 담도폐쇄여기간DNA병독감염지간존재상관성,기중이인거세포병독감염관계최위밀절.
Objective To establish the infection rates of hepatotropic DNA viruses in the liver tissues of biliary atresia patients.Methods Fluorescent quantitative PCR (FQ-PCR)was used to assess 5 viruses,including human cytomegalovirus(HCMV),adenovirus (ADV),EB virus (EBV),herpes simplex virus (HSV)and hepatitis B virus (HBV)in 85 biliarty atresia patients and 10 controls.Immunohistochemistry was carried out to assess the cytomegalovirus late proteins PP65 in paraffin sections.Results The fluorescence quantitative PCR result shows that among 85 patients,56(65.8%)were found to be positive for hepatotropic DNA virus.HCMV,ADV,EBV,HSV and HBV accounted for 51(60%),5(5.9%),3(3.5%),0(0%),and 0(0%)of the cases respectively.The biopsies of 3 patients were tested positive for two viruses simultaneously.They include two cases of HCMV in combination with ADV and one case of,ADV in combination with EBV.In the control group,virus detection was negative.Moreover,PP65 was located in the cytoplasm of infected cells such as liver cells,vascular endothelial cells,bile duct epithelial cells and inflammatory infiltrating cells.Conclusions Hepatotropic DNA viruses detection are associated with biliary atresia.Human cytomegalovirus has the strongest association with biliary atresia.