CAJ | 학술논문

目的筛选人皮肤黑素瘤组织中表达的已知has-microRNA.方法采用SBC microRNA芯片技术,将6例人皮肤黑素瘤组织总RNA分别与9例健康人色素痣总RNA混合物对比,筛选已知的468条has-microRNA表达情况.用qPCR分别检测这6例人皮肤黑素瘤组织中各候选has-microRNA的表达.将芯片和qPCR两种方法检测结果一致的候选has-microRNA确定为有意义的共同差异表达has-microRNA.结果 2倍以上差异表达miRNA占所检测miRNA的12.18%～86.33%,5倍以上差异表达miRNA占1.28%～19.02%,10倍以上差异表达miRNA占0.43%～5.34%.此6例人皮肤黑素瘤中miRNA-21明显上调表达,miRNA-320和miRNA-494明显下调表达.结论人皮肤黑素瘤组织中miRNA-21明显上调表达,miRNA-320和miRNA-494明显下调表达.
목적 사선인피부흑소류조직중표체적이지has-microRNA.방법 채용SBC microRNA심편기술,장6례인피부흑소류조직총RNA분별여9례건강인색소지총RNA혼합물대비,사선이지적468조has-microRNA표체정황.용qPCR분별검측저6례인피부흑소류조직중각후선has-microRNA적표체.장심편화qPCR량충방법 검측결과일치적후선has-microRNA학정위유의의적공동차이표체has-microRNA.결과 2배이상차이표체miRNA점소검측miRNA적12.18%～86.33%,5배이상차이표체miRNA점1.28%～19.02%,10배이상차이표체miRNA점0.43%～5.34%.차6례인피부흑소류중miRNA-21명현상조표체,miRNA-320화miRNA-494명현하조표체.결론 인피부흑소류조직중miRNA-21명현상조표체,miRNA-320화miRNA-494명현하조표체.
Objective To select microRNAs (miRNA) associated with human cutaneous malignant melanoma (MM). Methods Total RNA was extracted from 6 tissue samples of MM and 9 human control samples of pigmented nevi, and small RNAs of less than 200 bp were enriched, miRNA microarray was used to select differentially expressed miRNAs between tissue samples of MM and pigmented nevi from 468 candi-dates. The expression of differentially expressed miRNAs was confirmed by fluorescence based real-time quan-titative PCR (qPCR) in all of these samples. Those miRNAs that were identified as differentially expressed with both miRNA microarray and qPCR were considered as significant miRNAs. Results Between the tissue samples of MM and pigmented nevi, 12.18% to 86.33% of miRNAs differentially expressed by more than 2 folds, 1.28% to 19.02% by more than 5 folds, and 0.43% to 5.34% by more than 10 folds. The expression of miRNA-21 was obviously up-regulated, while that of miRNA-320 and miRNA-494 was down-regulated in the MM samples. Conclusion There is an increase in the expression of homo sapiens miRNA-21 but a decrease in that of miRNA-320 and miRNA-494 in MM tissues.