中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2009年
4期
318-322
,共5页
牛膺筠%刘夫玲%杨莹%袁春燕
牛膺筠%劉伕玲%楊瑩%袁春燕
우응균%류부령%양형%원춘연
视网膜母细胞瘤%新生血管化,病理性%缺氧诱导因子1,α亚基%血管内皮生长因子A
視網膜母細胞瘤%新生血管化,病理性%缺氧誘導因子1,α亞基%血管內皮生長因子A
시망막모세포류%신생혈관화,병이성%결양유도인자1,α아기%혈관내피생장인자A
Retinoblastoma%Neovascularization,pathologic%Hypoxia-inducible factorl,alpha submit%Vascular endothelial growth factor A
目的 探讨视网膜母细胞瘤(Rb)标本中是否存在血管生成拟态(VM),并分析VM与Rb临床病理特征及其与缺氧诱导因子-1αa(HIF-1α)、血管内皮生长因子(VEGF)蛋白表达的关系,进一步阐述VM的临床意义.方法 实验研究.收集60例Rb患者、10例正常人视网膜组织石蜡标本及相关临床病理资料,应用过碘酸-雪夫(PAS)与CD34双重染色以及免疫组织化学染色法检测60例Rb中是否存在VM和分布,观察与临床病理学特征的关系特点;检测HIF-1α、VEGF蛋白表达,分析Rb瘤组织中VM与HIF-1α、VEGF表达的关系;用CD34抗体进行肿瘤血管内皮染色,并计数肿瘤微血管密度(MVD).Rb和正常视网膜组织中的VM、HIF-1α、VEGF蛋白阳性表达的比较采用χ2检验;VM阳性组和VM阴性组的MVD值的比较采用q检验.结果 HE染色法观察到Rb中存在VM,其为Rb细胞构成的管腔样结构,无内皮细胞衬覆,腔内可见红细胞.CD34和PAS双重染色结合CD34、神经元特异性烯醇化酶(NSE)免疫组织化学染色进一步证明Rb中存在VM,60例Rb中VM阳性率为18.33%(11/60),其中随着R-E分级的增高,VM阳性表达率明显增加,差别具有统计学意义(χ2=8.861,P<0.05);分化型Rb中VM阳性率(4.34%)低于未分化型中VM阳性率(22.02%)(χ2=4.872,P<0.05);此外,HIF-1α、VEGF在Rb的VM形成过程中的阳性表达率依次为VM阳性组大于VM阴性组大于正常组;微血管密度计数高低与VM的表达有关,VM阴性组的MVD值(49.77±2.05)高于VM阳性组MVD值(36.53±1.15).结论 Rb中存在VM,且肿瘤恶性程度越高,形成VM能力越强.存在VM的Rb组织HIF-1α、VEGF高表达,提示HIF-1α、VEGF在VM的形成中可能起促进作用.
目的 探討視網膜母細胞瘤(Rb)標本中是否存在血管生成擬態(VM),併分析VM與Rb臨床病理特徵及其與缺氧誘導因子-1αa(HIF-1α)、血管內皮生長因子(VEGF)蛋白錶達的關繫,進一步闡述VM的臨床意義.方法 實驗研究.收集60例Rb患者、10例正常人視網膜組織石蠟標本及相關臨床病理資料,應用過碘痠-雪伕(PAS)與CD34雙重染色以及免疫組織化學染色法檢測60例Rb中是否存在VM和分佈,觀察與臨床病理學特徵的關繫特點;檢測HIF-1α、VEGF蛋白錶達,分析Rb瘤組織中VM與HIF-1α、VEGF錶達的關繫;用CD34抗體進行腫瘤血管內皮染色,併計數腫瘤微血管密度(MVD).Rb和正常視網膜組織中的VM、HIF-1α、VEGF蛋白暘性錶達的比較採用χ2檢驗;VM暘性組和VM陰性組的MVD值的比較採用q檢驗.結果 HE染色法觀察到Rb中存在VM,其為Rb細胞構成的管腔樣結構,無內皮細胞襯覆,腔內可見紅細胞.CD34和PAS雙重染色結閤CD34、神經元特異性烯醇化酶(NSE)免疫組織化學染色進一步證明Rb中存在VM,60例Rb中VM暘性率為18.33%(11/60),其中隨著R-E分級的增高,VM暘性錶達率明顯增加,差彆具有統計學意義(χ2=8.861,P<0.05);分化型Rb中VM暘性率(4.34%)低于未分化型中VM暘性率(22.02%)(χ2=4.872,P<0.05);此外,HIF-1α、VEGF在Rb的VM形成過程中的暘性錶達率依次為VM暘性組大于VM陰性組大于正常組;微血管密度計數高低與VM的錶達有關,VM陰性組的MVD值(49.77±2.05)高于VM暘性組MVD值(36.53±1.15).結論 Rb中存在VM,且腫瘤噁性程度越高,形成VM能力越彊.存在VM的Rb組織HIF-1α、VEGF高錶達,提示HIF-1α、VEGF在VM的形成中可能起促進作用.
목적 탐토시망막모세포류(Rb)표본중시부존재혈관생성의태(VM),병분석VM여Rb림상병리특정급기여결양유도인자-1αa(HIF-1α)、혈관내피생장인자(VEGF)단백표체적관계,진일보천술VM적림상의의.방법 실험연구.수집60례Rb환자、10례정상인시망막조직석사표본급상관림상병리자료,응용과전산-설부(PAS)여CD34쌍중염색이급면역조직화학염색법검측60례Rb중시부존재VM화분포,관찰여림상병이학특정적관계특점;검측HIF-1α、VEGF단백표체,분석Rb류조직중VM여HIF-1α、VEGF표체적관계;용CD34항체진행종류혈관내피염색,병계수종류미혈관밀도(MVD).Rb화정상시망막조직중적VM、HIF-1α、VEGF단백양성표체적비교채용χ2검험;VM양성조화VM음성조적MVD치적비교채용q검험.결과 HE염색법관찰도Rb중존재VM,기위Rb세포구성적관강양결구,무내피세포츤복,강내가견홍세포.CD34화PAS쌍중염색결합CD34、신경원특이성희순화매(NSE)면역조직화학염색진일보증명Rb중존재VM,60례Rb중VM양성솔위18.33%(11/60),기중수착R-E분급적증고,VM양성표체솔명현증가,차별구유통계학의의(χ2=8.861,P<0.05);분화형Rb중VM양성솔(4.34%)저우미분화형중VM양성솔(22.02%)(χ2=4.872,P<0.05);차외,HIF-1α、VEGF재Rb적VM형성과정중적양성표체솔의차위VM양성조대우VM음성조대우정상조;미혈관밀도계수고저여VM적표체유관,VM음성조적MVD치(49.77±2.05)고우VM양성조MVD치(36.53±1.15).결론 Rb중존재VM,차종류악성정도월고,형성VM능력월강.존재VM적Rb조직HIF-1α、VEGF고표체,제시HIF-1α、VEGF재VM적형성중가능기촉진작용.
Objective To explore if vasculogenic mimicry (VM) exists in retinoblastoma (Rb)and to explore the clinical significance of VM. Methods It was an experimental study. Sixty Rb specimens with complete clinical and prognostic data were collected. Periodic acid-Schif staining and immunohistochemical staining of CD34 were conducted to explore if VM exists in those Rb specimens. The expression of HIF-1α and VEGF were examined by immunohistochemical staining. The expression of CD34 endothelial antigen was used to label the neo-microvessels. Microvesseldensity (MVD) in retinoblastoma tissues was calculated. Results In HE staining slides, VM was present in Rb specimens and was formed by tumor cells but not endothelial cells. Red blood cells were present in the VM. VM existed in 18.33% (11/60) of the Rb specimens. Low R-E graded Rb specimens exhibited a higher VM positive rate than that in the high R-E graded Rb (χ2=8.861 ,P <0.05). The positive rate of VM was 4.34% in differentiated type of Rb and was 22.02% in undifferentiated type of Rb (χ2= 4.872, P <0.0). HIF-1α and VEGF expressions in Rb with VM were significantly greater than those in Rb without VM (P = 0.001). The density of endothelial vessels correlated with VM. The mean MVD was 49.77±2. 05 in Rb without VM and 36.53±1.15 in Rb with VM (P <0.05). Conclusions VM exists in Rb. Highly differentiated Rb exhibits more VM than that in less differentiated Rb. Expression of HIF-1α and VEGF is greater in Rb with VM, indicating that these factors may stimulate the occurrence of VM.
