CAJ | 학술논문

目的用流式细胞仪分析肺癌患者外周血循环中的癌细胞。方法外周血经Ficoll梯度离心分离单核细胞组分，后者用CD45、细胞角蛋白（CK）及肺癌特异性抗体（2F7／S5A）染色后，应用流式细胞仪检测CD45－CK＋2F7／S5A＋细胞。结果检测了165例肺癌患者外周血，发现50例（30.30％）患者外周血癌细胞呈阳性。其中非小细胞肺癌阳性率为30.67％(45／150)，小细胞肺癌为33.33％。阳性率与患者的病理分期有显著相关性（P＜0.05）。结论利用流式细胞仪分析检测肺癌患者外周血循环中的癌细胞不仅有助于临床病理分期，而且对预测肺癌转移潜能具有应用价值。
목적 용류식세포의분석폐암환자외주혈순배중적암세포。방법 외주혈경Ficoll제도리심분리단핵세포조분，후자용CD45、세포각단백（CK）급폐암특이성항체（2F7／S5A）염색후，응용류식세포의검측CD45－CK＋2F7／S5A＋세포。결과 검측료165례폐암환자외주혈，발현50례（30.30％）환자외주혈암세포정양성。기중비소세포폐암양성솔위30.67％(45／150)，소세포폐암위33.33％。양성솔여환자적병리분기유현저상관성（P＜0.05）。결론 이용류식세포의분석검측폐암환자외주혈순배중적암세포불부유조우림상병리분기，이차대예측폐암전이잠능구유응용개치。
Objective To analyze circulating lung cancer cells in theperipheral blood in patients with lung cancer by flow cytometry (FCM). Methods The monocyte fraction in peripheral blood was isolated by Ficoll-Hypaque gradient centrifugation. The cells obtained were labeled with antibodies against CD45, cytokeratin (CK) and antigen (2F7／S5A). The CD45－CK＋2F7／S5A＋cells were analyzed by FCM. Results Fifty cases out of 165 patients with lung cancer (30.30％) were found to have cancer cells in the peripheral blood. Positive rate in non-small cell lung cancer was 30.67％(45／150) and that in small cell lung cancer was 33.33％(5／15) respectively. Meanwhile, there was distinct correlation between detective rate of cancer cells in the peripheral blood and pathological stage（P＜0.05）. Conclusion Examination of lung cancer presented in the peripheral blood by FCM might be helpful for staging and finding metastatic potential of lung cancer.