CAJ | 학술논문

目的:分离及鉴定来自于新疆塔城民间自制酸梅酱中的酵母菌.方法:用NL1/NL4引物对扩增酵母菌株的26S rDNA D1/D2区,测序结果进行序列分析,用Neighbour-joining(N-J)方法构建系统发育进化树,同时结合酵母的传统形态学鉴定对菌株进行鉴定.结果:26S rDNA D1/D2区序列分析表明菌株KKS与Metschnikowia aff.fructicola D3895相近,相似率为99.2%.在N-J法构建的系统发育进化树中,菌株KKS与Metschnikowia aff.fructicola聚类在同一分枝上.结论:从新疆塔城民间自制酸梅酱中分离得到一株酵母菌并将该菌株鉴定为Metschnikowia aff.fructicola.
목적:분리급감정래자우신강탑성민간자제산매장중적효모균.방법:용NL1/NL4인물대확증효모균주적26S rDNA D1/D2구,측서결과진행서렬분석,용Neighbour-joining(N-J)방법구건계통발육진화수,동시결합효모적전통형태학감정대균주진행감정.결과:26S rDNA D1/D2구서렬분석표명균주KKS여Metschnikowia aff.fructicola D3895상근,상사솔위99.2%.재N-J법구건적계통발육진화수중,균주KKS여Metschnikowia aff.fructicola취류재동일분지상.결론:종신강탑성민간자제산매장중분리득도일주효모균병장해균주감정위Metschnikowia aff.fructicola.
Objective: Yeast from jam of Xinjiang Tacheng' s plum was isolated and identified. Method: 26S rDNA D1/D2 domain sequence for yeast strain was amplified by using primer pairs NL1/NL4, the acquired data were used to analyse sequence, and using neighbour - joining method to construct phylogenetic tree. To confirm identification, the morphological and physiological properties were also examined. Result: The sequence analysis of 26S rDNA D1/D2 showed that strain KKS was closely related to Metschnikowia aff. fructicola D3895, with the similarity of 99.2%. In the phylogenetic tree drawn from neighbor-joining method, strain KKS and Metschnikowia aff. fructicola were in the same branch. Conclusion: One yeast strain from jam of Xinjiang Tacheng' s plum was isolated and identified to Metschnikowia aff. fructicola.