CAJ | 학술논문

目的探讨地塞米松对支气管哮喘(简称哮喘)豚鼠模型白介素4(IL-4)、IL-5水平及气道高反应性(airway hyperreactivity,AHR)的影响,为激素治疗哮喘提供理论依据.方法实验分为3组:正常对照组、哮喘模型组和地塞米松治疗组,每组各10只豚鼠.用卵白蛋白腹腔注射致敏和雾化吸入激发建立哮喘豚鼠模型,三通管做气管插管进行机械通气,测定气道内压力以反映气道反应性,酶联免疫吸附试验(ELISA)检测豚鼠血清中IL-4、IL-5含量.结果哮喘模型组豚鼠血清IL-4、IL-5含量,分别为(38.2±3.4)ng/L和(344.4±21.8)ng/L,显著高于正常对照组,分别为(19.8±1.2)ng/L和(77.8±26.0)ng/L(P＜0.01);地塞米松组IL-4、IL-5含量,分别为(22.0±1.8) ng/L和(234.6±35.1)ng/L,均明显低于哮喘模型组(P＜0.01).哮喘豚鼠模型组气道反应性(0.013±0.014) g/L与正常对照组(0.168±0.186) g/L比较显著升高(P＜0.01);地塞米松组气道反应性(0.144±0.154) g/L与哮喘模型组比较显著降低(P＜0.01),与正常对照组比较差异无统计学意义(P＞0.05).结论地塞米松能有效降低哮喘豚鼠AHR,其机制可能是抑制哮喘豚鼠体内IL-4、IL-5的生成.
목적 탐토지새미송대지기관효천(간칭효천)돈서모형백개소4(IL-4)、IL-5수평급기도고반응성(airway hyperreactivity,AHR)적영향,위격소치료효천제공이론의거.방법 실험분위3조:정상대조조、효천모형조화지새미송치료조,매조각10지돈서.용란백단백복강주사치민화무화흡입격발건립효천돈서모형,삼통관주기관삽관진행궤계통기,측정기도내압력이반영기도반응성,매련면역흡부시험(ELISA)검측돈서혈청중IL-4、IL-5함량.결과 효천모형조돈서혈청IL-4、IL-5함량,분별위(38.2±3.4)ng/L화(344.4±21.8)ng/L,현저고우정상대조조,분별위(19.8±1.2)ng/L화(77.8±26.0)ng/L(P＜0.01);지새미송조IL-4、IL-5함량,분별위(22.0±1.8) ng/L화(234.6±35.1)ng/L,균명현저우효천모형조(P＜0.01).효천돈서모형조기도반응성(0.013±0.014) g/L여정상대조조(0.168±0.186) g/L비교현저승고(P＜0.01);지새미송조기도반응성(0.144±0.154) g/L여효천모형조비교현저강저(P＜0.01),여정상대조조비교차이무통계학의의(P＞0.05).결론 지새미송능유효강저효천돈서AHR,기궤제가능시억제효천돈서체내IL-4、IL-5적생성.
Objective To explore the effects of dexamethasone on interleukin-4 (IL-4) ,IL-5 in serum and airway hyperresponsiveness(AHR) of asthmatic model in guinea pig and to provide theoretical basis for the treatment of bronchial asthma(asthma). Methods This experiment included three groups:normal control group,asthmatic model group and dexamethasone treatment group,involving 10 guinea pigs in each group.Asthmatic models in guinea pigs were constructed by immunization of intraperitoneal injection with saline ovalbumin(OVA) and challenge of inspiration with nebulized OVA. A three-way cannula was inserted into the lumen of the trachea for mechanical ventilation and the intratracheal pressure (IP) was measured simultaneously for reflecting airway responsiveness. The levels of IL-4, IL-5 in serum of guinea pigs were determined using enzyme-linked immunosorbent assay (ELISA). Results The levels of IL-4, IL-5 of asthmatic model group (38.2±3.4) ng/L and (344.4±21.8) ng/L were significantly higher than those of normal control group (19.8±1.2) ng/L and (77.8±26.0) ng/L( P＜0.01). The levels of IL-4,IL-5 of dexamethasone group (22. 0±1. 8) ng/L and (234.6±35.1) ng/L were significantly lower than those of asthmatic model group( P＜0.01). The airway responsiveness of asthmatic model group (0. 013±0.014) g/L was significantly higher than that of normal control group (0. 168±0. 186) g/L (P＜0.01). Compared with that of asthmatic model group, the airway responsiveness of dexamethasone group (0. 144±0. 154) g/L decreased significantly( P ＜ 0.01), but they did not have any statistical significance from that of normal control group( P ＞ 0.05). Conclusions Dexamethasone could efficiently decrease the AHR of asthmatic guinea pig. The mechanism may be that dexamethasone could prevent the production of IL-4, IL-5 in asthmatic guinea pig.