CAJ | 학술논문

目的应用分析化学技术建立正常儿童粪便样本中短链脂肪酸的指纹图谱,并分析其在功能性便秘(FC)儿童中的变化,探讨肠道短链脂肪酸变化在儿童功能性便秘中的作用.方法研究对象分为对照组和便秘组,对照组35例,平均年龄6岁,便秘组15例,平均年龄6.5岁,均符合儿童功能性便秘罗马Ⅲ标准.取所有儿童粪便样本提取短链脂肪酸(SCFA),应用气相色谱技术(GC)对SCFA的含量进行定量检测.结果对照组儿童每克干粪中SCFA的含量为(51.89±17.62)mg/g,其中各SCFA的含量依次为:乙酸,丁酸,丙酸,异丁酸和异戊酸;与对照组相比,便秘儿童每克干粪中SCFA总量明显减少,差异有统计学意义(P<11.01);其中乙酸,丙酸和丁酸的含量减少,异丁酸和异戊酸含量增加,其含量顺序依次为:异丁酸最多,异戊酸次之,乙酸,丙酸和丁酸含量最低;便秘组粪便中sCFA浓度(87.29±33.42)mg/L较时照组的(17.36±8.12)mg/L增高,差异有统计学意义(P<0.01);结论功能性便秘儿童粪便中的短链脂肪酸含量减少,比例失调,但浓度升高,功能性便秘的产生可能与肠道内短链脂肪酸吸收过度以及局部的短链脂肪酸浓度升高有关.
목적 응용분석화학기술건립정상인동분편양본중단련지방산적지문도보,병분석기재공능성편비(FC)인동중적변화,탐토장도단련지방산변화재인동공능성편비중적작용.방법 연구대상분위대조조화편비조,대조조35례,평균년령6세,편비조15례,평균년령6.5세,균부합인동공능성편비라마Ⅲ표준.취소유인동분편양본제취단련지방산(SCFA),응용기상색보기술(GC)대SCFA적함량진행정량검측.결과 대조조인동매극간분중SCFA적함량위(51.89±17.62)mg/g,기중각SCFA적함량의차위:을산,정산,병산,이정산화이무산;여대조조상비,편비인동매극간분중SCFA총량명현감소,차이유통계학의의(P<11.01);기중을산,병산화정산적함량감소,이정산화이무산함량증가,기함량순서의차위:이정산최다,이무산차지,을산,병산화정산함량최저;편비조분편중sCFA농도(87.29±33.42)mg/L교시조조적(17.36±8.12)mg/L증고,차이유통계학의의(P<0.01);결론공능성편비인동분편중적단련지방산함량감소,비례실조,단농도승고,공능성편비적산생가능여장도내단련지방산흡수과도이급국부적단련지방산농도승고유관.
Objective To investigate the formation and proportion of the short-chain fatty acids (SCFA) in normal and functional constipation (FC) children.Methods Fifty children were divided into control group (15) and FC group (35).SCFA were extracted from children's stool, and quantitatively analyzed by gas chromatography (GC).Results Of control group, SCFA of stool was 51.89±17.62 (mg/g);among the SCFA, acetic acid was the richest, butyric acid was the second richest, followed by propanoic acid third, isobutyric acid fourth, and isopentoic acid the last.Compared with the SCFA of control group, the stool SCFA in FC group significantly decreased; among the SCFA, isobutyric acid was the richest, isopentoic acid second, and then came propanoic acid, acetic acid, and butyric acid.The content of acetic acid, butyric acid and propanoic acid decreased, while the isobutyric acid and isopentoic acid increased in FC group.The concentration of SCFA in FC group was 87.29±33.42 mg/L, which was significantly higher than 17.36±8.12 mg/L of the control group (P<0.01).Conclusions The stool SCFA increases and disproportions in FC patients, which may contribute to the pathogenesis of FC in children.