中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2011年
8期
685-687
,共3页
吴高义%陈磊%孙晶%黄飞%朱国雄%李冬临
吳高義%陳磊%孫晶%黃飛%硃國雄%李鼕臨
오고의%진뢰%손정%황비%주국웅%리동림
白介素-1%白介素-6%颞下颌关节%心理应激
白介素-1%白介素-6%顳下頜關節%心理應激
백개소-1%백개소-6%섭하합관절%심리응격
Interlukin-1%Interlukin-6%Tempommandibular joint%Psychological stress
目的 通过对心理应激大鼠颞下颌关节(tempommandibular joint,TMJ)的形态、生理学评估及相关炎性因子的检测,探讨心理应激对TMJ的可能致病机制.方法 采用交流箱心理应激动物模型,检测空白对照组、心理应激组(PS组)、心理应激加地西泮组(PS+DI组)大鼠血清中Cor和ACTH水平;运用RT-PCR技术检测心理应激不同时期大鼠TMJ内炎性因子IL-1和IL-6的表达变化.结果 空白对照组血清中Cor和ACTH值与PS组有显著差异(P＜0.01),空白对照组血清中Cor和ACTH值与PS+DI组无显著差异(P＞0.05);空白对照组IL-1(分别为(0.453±0.021)mg/L,(0.439±0.028)mg/L,0.454±0.023)mg/L、IL-6[分别为(0.525±0.028)mg/L,(0.515±0.028)mg/L,(0.518±0.022)mg/L]与PS组IL-1[分别为(0.981±0.024)mg/L,(0.746±0.017)mg/L,(0.510±0.016)mg/L]、IL-6[分别为(0.820±0.023)mg/L,(0.694±0.019)mg/L,(0.579±0.015)mg/L]有显著差异(P＜0.05),与PS+DI组IL-1[分别为(0.549±0.014)mg/L,(0.498±0.014)mg/L,(0.444±0.022)mg/L]、IL-6[分别为(0.599±0.015)mg/L,(0.541±0.015)mg/L,(0.487±0.008)mg/L]差异无显著性(P＞0.05).结论 心理应激可能在TMD形成过程中起着重要作用.
目的 通過對心理應激大鼠顳下頜關節(tempommandibular joint,TMJ)的形態、生理學評估及相關炎性因子的檢測,探討心理應激對TMJ的可能緻病機製.方法 採用交流箱心理應激動物模型,檢測空白對照組、心理應激組(PS組)、心理應激加地西泮組(PS+DI組)大鼠血清中Cor和ACTH水平;運用RT-PCR技術檢測心理應激不同時期大鼠TMJ內炎性因子IL-1和IL-6的錶達變化.結果 空白對照組血清中Cor和ACTH值與PS組有顯著差異(P＜0.01),空白對照組血清中Cor和ACTH值與PS+DI組無顯著差異(P＞0.05);空白對照組IL-1(分彆為(0.453±0.021)mg/L,(0.439±0.028)mg/L,0.454±0.023)mg/L、IL-6[分彆為(0.525±0.028)mg/L,(0.515±0.028)mg/L,(0.518±0.022)mg/L]與PS組IL-1[分彆為(0.981±0.024)mg/L,(0.746±0.017)mg/L,(0.510±0.016)mg/L]、IL-6[分彆為(0.820±0.023)mg/L,(0.694±0.019)mg/L,(0.579±0.015)mg/L]有顯著差異(P＜0.05),與PS+DI組IL-1[分彆為(0.549±0.014)mg/L,(0.498±0.014)mg/L,(0.444±0.022)mg/L]、IL-6[分彆為(0.599±0.015)mg/L,(0.541±0.015)mg/L,(0.487±0.008)mg/L]差異無顯著性(P＞0.05).結論 心理應激可能在TMD形成過程中起著重要作用.
목적 통과대심리응격대서섭하합관절(tempommandibular joint,TMJ)적형태、생이학평고급상관염성인자적검측,탐토심리응격대TMJ적가능치병궤제.방법 채용교류상심리응격동물모형,검측공백대조조、심리응격조(PS조)、심리응격가지서반조(PS+DI조)대서혈청중Cor화ACTH수평;운용RT-PCR기술검측심리응격불동시기대서TMJ내염성인자IL-1화IL-6적표체변화.결과 공백대조조혈청중Cor화ACTH치여PS조유현저차이(P＜0.01),공백대조조혈청중Cor화ACTH치여PS+DI조무현저차이(P＞0.05);공백대조조IL-1(분별위(0.453±0.021)mg/L,(0.439±0.028)mg/L,0.454±0.023)mg/L、IL-6[분별위(0.525±0.028)mg/L,(0.515±0.028)mg/L,(0.518±0.022)mg/L]여PS조IL-1[분별위(0.981±0.024)mg/L,(0.746±0.017)mg/L,(0.510±0.016)mg/L]、IL-6[분별위(0.820±0.023)mg/L,(0.694±0.019)mg/L,(0.579±0.015)mg/L]유현저차이(P＜0.05),여PS+DI조IL-1[분별위(0.549±0.014)mg/L,(0.498±0.014)mg/L,(0.444±0.022)mg/L]、IL-6[분별위(0.599±0.015)mg/L,(0.541±0.015)mg/L,(0.487±0.008)mg/L]차이무현저성(P＞0.05).결론 심리응격가능재TMD형성과정중기착중요작용.
Objective To investigate the possible mechanisms of the attribution of psychological stress to the temporomandibular joint disorder(TMD) ,through the evaluation of the animal model and detection of the proinflammatory cytokines in the TMJ.Methods The animal models of communication box were built to mimic the psychological stress.The concentration of the serum Cor and ACTH was detected in the control group, Psychological Stress group ( PS group), and diazepam ( anti-anxiety drug) group ( PS + DI group).The expression of the proinflammatory cytokines IL-1 and IL-6 in the rat TMJ in the different phases of psychologicalstress was detected by RT-PCR.Results The results of the serum concen- tration of Cor and ACTH showed that there was significant difference between the control group and the PS group(P＜0.01 ) ,while no significant difference between the control group and the PS + DI group(P＞0.05).The expressions of IL-1 and IL-6 were comapared in all group.The expressions of IL-1 in CON group were (0.453± 0.021 ) mg/L, (0.439 ± 0.028 ) mg/L and (0.454 ± 0.023 )mg/L.These values were markedly increased compared with those of the PS group(0.981 ±0.024)mg/L, (0.746±0.017)mg/L and (0.510 ±0.016)mg/L respectively, P＜0.01 ) ,but no significant differences compared with PS + DI group(0.549 ± 0.014 ) mg/L, ( 0.498 ± 0.014 ) mg/L and ( 0.444 ± 0.022 ) mg/L respectively, P ＞ 0.05).Similar changes were observed in expressions of IL-6.The expressions of IL-6 in the CON rats were (0.525 ±0.028)mg/L,(0.515 ±0.028)mg/L and (0.518 ±0.022)mg/L,respectively,while those of PS group were(0.820 ± 0.023 ) mg/L, (0.694 ± 0.019 ) mg/L and (0.579 ± 0.015 ) mg/L, respectively, which were significan- tly higher in the PS groups(P＜ 0.05 ).But there were no significant differences between CON group and PS + DI group( (0.599 ±0.015)mg/L, (0.541 ±0.015)mg/L, (0.487 ±0.008)mg/L respectively, P＞0.05).Conclusion The psychological stress can play important role in the formation of TMD.
