中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
11期
1374-1376
,共3页
刘燕%李磊强%李云露%杜晨露%邵云涛%郑新春%张翠英
劉燕%李磊彊%李雲露%杜晨露%邵雲濤%鄭新春%張翠英
류연%리뢰강%리운로%두신로%소운도%정신춘%장취영
硫辛酸%肾%再灌注损伤%心肌%细胞凋亡
硫辛痠%腎%再灌註損傷%心肌%細胞凋亡
류신산%신%재관주손상%심기%세포조망
Thioctic acid%Kidney%Reperfusion injury%Myocardium%Apoptosis
目的 评价α-硫辛酸对肾缺血再灌注损伤大鼠心肌细胞凋亡的影响.方法 健康雄性SD大鼠36只,体重250～280 g,采用随机数字表法,将其随机分为3组:假手术组(S组)、肾缺血再灌注组(I/R组)和α-硫辛酸组(L组),每组12只.I/R组和L组采用夹闭双侧肾动、静脉45 min后恢复灌注的方法制备大鼠肾缺血再灌注损伤模型,S组不夹闭双侧肾蒂.L组于夹闭前20 min和再灌注前20 min分别尾静脉注射α-硫辛酸30 mg/kg,I/R组分别尾静脉注射等容量溶剂(35％聚乙二醇+60％生理盐水+5％乙醇).于再灌注24h抽取腹主动脉血,测定血清肌酐(Cr)和MDA浓度,随后处死大鼠取心脏,测定心肌MDA含量和SOD活性,采用流式细胞术检测心肌细胞凋亡率,采用免疫组化法测定心肌细胞Bcl-2及Bax的表达,计算Bcl-2/Bax比值.结果 与S组相比,I/R组和L组血清Cr和MDA浓度、心肌MDA含量和心肌细胞凋亡率升高,SOD活性降低,I/R组Bcl-2/Bax比值降低(P＜0.05)；与JR组相比,L组血清Cr和MDA浓度、心肌MDA含量和心肌细胞凋亡率降低,SOD活性升高,Bcl-2/Bax比值升高(P＜0.05).结论 α-硫辛酸可减轻肾缺血再灌注损伤大鼠心肌损伤,与抑制心肌细胞凋亡有关.
目的 評價α-硫辛痠對腎缺血再灌註損傷大鼠心肌細胞凋亡的影響.方法 健康雄性SD大鼠36隻,體重250～280 g,採用隨機數字錶法,將其隨機分為3組:假手術組(S組)、腎缺血再灌註組(I/R組)和α-硫辛痠組(L組),每組12隻.I/R組和L組採用夾閉雙側腎動、靜脈45 min後恢複灌註的方法製備大鼠腎缺血再灌註損傷模型,S組不夾閉雙側腎蒂.L組于夾閉前20 min和再灌註前20 min分彆尾靜脈註射α-硫辛痠30 mg/kg,I/R組分彆尾靜脈註射等容量溶劑(35％聚乙二醇+60％生理鹽水+5％乙醇).于再灌註24h抽取腹主動脈血,測定血清肌酐(Cr)和MDA濃度,隨後處死大鼠取心髒,測定心肌MDA含量和SOD活性,採用流式細胞術檢測心肌細胞凋亡率,採用免疫組化法測定心肌細胞Bcl-2及Bax的錶達,計算Bcl-2/Bax比值.結果 與S組相比,I/R組和L組血清Cr和MDA濃度、心肌MDA含量和心肌細胞凋亡率升高,SOD活性降低,I/R組Bcl-2/Bax比值降低(P＜0.05)；與JR組相比,L組血清Cr和MDA濃度、心肌MDA含量和心肌細胞凋亡率降低,SOD活性升高,Bcl-2/Bax比值升高(P＜0.05).結論 α-硫辛痠可減輕腎缺血再灌註損傷大鼠心肌損傷,與抑製心肌細胞凋亡有關.
목적 평개α-류신산대신결혈재관주손상대서심기세포조망적영향.방법 건강웅성SD대서36지,체중250～280 g,채용수궤수자표법,장기수궤분위3조:가수술조(S조)、신결혈재관주조(I/R조)화α-류신산조(L조),매조12지.I/R조화L조채용협폐쌍측신동、정맥45 min후회복관주적방법제비대서신결혈재관주손상모형,S조불협폐쌍측신체.L조우협폐전20 min화재관주전20 min분별미정맥주사α-류신산30 mg/kg,I/R조분별미정맥주사등용량용제(35％취을이순+60％생리염수+5％을순).우재관주24h추취복주동맥혈,측정혈청기항(Cr)화MDA농도,수후처사대서취심장,측정심기MDA함량화SOD활성,채용류식세포술검측심기세포조망솔,채용면역조화법측정심기세포Bcl-2급Bax적표체,계산Bcl-2/Bax비치.결과 여S조상비,I/R조화L조혈청Cr화MDA농도、심기MDA함량화심기세포조망솔승고,SOD활성강저,I/R조Bcl-2/Bax비치강저(P＜0.05)；여JR조상비,L조혈청Cr화MDA농도、심기MDA함량화심기세포조망솔강저,SOD활성승고,Bcl-2/Bax비치승고(P＜0.05).결론 α-류신산가감경신결혈재관주손상대서심기손상,여억제심기세포조망유관.
Objective To evaluate the effect of alpha-lipoic acid (ALA) on cardiomyocyte apoptosis following renal ischemia-reperfusion injury(RIRI) in rats.Methods Thirty-six male SD rats weighing 250-280 g were randomly divided into 3 groups ( n =12 each): group sham operation (group S) ; group I/R and group I/R + ALA ( group L).The model of RIRI was produced by occlusion of renal artery and vein for 45 min followed by 24 h reperfusion,in group S the renal pedicles were exposed but not occluded.In group L ALA infusion (30 mg/kg) was given via tail vein at 20 mln before ischemia and at 20 min before reperfusion,while in group I/R the equal volume of solution (35％ polyethylene glycol + 60％ physiological saline + 5％ ethanol) was infused instead of ALA.The animals were saerificed at the end of 24 h of reperfusion,blood samples were taken for detecting concentrations of serum creatinine (Cr) and malondialdehyde (MDA).Then the hearts were immediately removed for determination of SOD activity,MDA content,cardiomyocyte apoptosis (flow cytometry) and Bcl-2/Bax ratio (immunohistology).Results Serum Cr concentration,serum and myocardium MDA levels and cardiomyocyte apoptosis were significantly increased after RIRI in groups I/R and L as compared with group S ( P ＜ 0.05).ALA treatment significantly decreased serum Cr concentration,serum and myocardium MDA levels,cardiomyocyte apoptosis and increased SOD activity and Bcl-2/Bax ratio ( P ＜ 0.05).Conclusion ALA can attenuate myocardium injury by inhibiting cardiomyocyte apoptosis following RIRI in rats.
