中华烧伤杂志
中華燒傷雜誌
중화소상잡지
16
2011年
5期
358-362
,共5页
徐庆连%蔡晨%戚伟伟%夏正国%唐益忠
徐慶連%蔡晨%慼偉偉%夏正國%唐益忠
서경련%채신%척위위%하정국%당익충
烧伤%脂肪酸类,不饱和%肿瘤坏死因子α%巨噬细胞游走抑制因子%肺%炎症%NF-KB%巨噬细胞炎性蛋白质1
燒傷%脂肪痠類,不飽和%腫瘤壞死因子α%巨噬細胞遊走抑製因子%肺%炎癥%NF-KB%巨噬細胞炎性蛋白質1
소상%지방산류,불포화%종류배사인자α%거서세포유주억제인자%폐%염증%NF-KB%거서세포염성단백질1
Burns%Fatty acids,unsaturated%Tumor necrosis factor-alpha%Macrophage migration inhibitory factors%Lung%Inflammation%Nuclear factor kappa B%Macrophage inflammatory protein 1
目的 观察ω-3多不饱和脂肪酸(PUFA)对严重烫伤大鼠肺部炎症的影响并探讨其机制.方法 72只成年SD大鼠按照随机数字表法分成3组:假伤组(A组,8只)、ω-3 PUFA治疗组(B组,32只)、中长链脂肪酸治疗组(C组,32只).A组大鼠浸入32℃水中模拟烫伤,伤后不予其他处理；B、C组制成30％ TBSAⅢ度烫伤,伤后5 min内,经尾静脉分别注射100g/L ω-3 PUFA(1 mL/kg)和200 g/L中长链脂肪酸注射液(2 mL/kg),两者热量等值,之后每天注射1次,连续10d.检测A组大鼠伤后即刻及B、C组伤后1、4、7、10 d血清TNF-α、肺组织匀浆巨噬细胞炎症蛋白1α(MIP-1α)水平,免疫组织化学染色法观察肺组织NF-KB p65及巨噬细胞游走抑制因子(MIF)表达情况.对实验数据进行t检验.结果 伤后所有时相点3组大鼠各项检测指标比较,均为B、C组明显高于A组,t值为3.411～8.782,P值均小于0.01.伤后4、7、10 d,B组各项指标水平明显低于C组,t值为2.321～2.785,P值均小于0.05.A组TNF-α为(0.96±0.32) ng/mL、MIP-1α为(76±16) pg/mL、NF-kB p65为(0.24±0.03)分、MIF为(1.31±0.03)分；B组以上指标伤后7d达峰值,分别为(2.43±0 32) ng/mL、(210±56) pg/mL、(4.23±2.15)分、(4.69±1.83)分；C组以上指标伤后7d为(3.15±0 54) ng/mL、(274±64) pg/mL、(5.15±2.31)分、(5.37±2.16)分.结论 ω-3 PUFA能降低烫伤大鼠血清TNF-α含量和肺组织MIP-1α、NF-KB p65、MIF水平,对肺组织有一定保护作用.
目的 觀察ω-3多不飽和脂肪痠(PUFA)對嚴重燙傷大鼠肺部炎癥的影響併探討其機製.方法 72隻成年SD大鼠按照隨機數字錶法分成3組:假傷組(A組,8隻)、ω-3 PUFA治療組(B組,32隻)、中長鏈脂肪痠治療組(C組,32隻).A組大鼠浸入32℃水中模擬燙傷,傷後不予其他處理；B、C組製成30％ TBSAⅢ度燙傷,傷後5 min內,經尾靜脈分彆註射100g/L ω-3 PUFA(1 mL/kg)和200 g/L中長鏈脂肪痠註射液(2 mL/kg),兩者熱量等值,之後每天註射1次,連續10d.檢測A組大鼠傷後即刻及B、C組傷後1、4、7、10 d血清TNF-α、肺組織勻漿巨噬細胞炎癥蛋白1α(MIP-1α)水平,免疫組織化學染色法觀察肺組織NF-KB p65及巨噬細胞遊走抑製因子(MIF)錶達情況.對實驗數據進行t檢驗.結果 傷後所有時相點3組大鼠各項檢測指標比較,均為B、C組明顯高于A組,t值為3.411～8.782,P值均小于0.01.傷後4、7、10 d,B組各項指標水平明顯低于C組,t值為2.321～2.785,P值均小于0.05.A組TNF-α為(0.96±0.32) ng/mL、MIP-1α為(76±16) pg/mL、NF-kB p65為(0.24±0.03)分、MIF為(1.31±0.03)分；B組以上指標傷後7d達峰值,分彆為(2.43±0 32) ng/mL、(210±56) pg/mL、(4.23±2.15)分、(4.69±1.83)分；C組以上指標傷後7d為(3.15±0 54) ng/mL、(274±64) pg/mL、(5.15±2.31)分、(5.37±2.16)分.結論 ω-3 PUFA能降低燙傷大鼠血清TNF-α含量和肺組織MIP-1α、NF-KB p65、MIF水平,對肺組織有一定保護作用.
목적 관찰ω-3다불포화지방산(PUFA)대엄중탕상대서폐부염증적영향병탐토기궤제.방법 72지성년SD대서안조수궤수자표법분성3조:가상조(A조,8지)、ω-3 PUFA치료조(B조,32지)、중장련지방산치료조(C조,32지).A조대서침입32℃수중모의탕상,상후불여기타처리；B、C조제성30％ TBSAⅢ도탕상,상후5 min내,경미정맥분별주사100g/L ω-3 PUFA(1 mL/kg)화200 g/L중장련지방산주사액(2 mL/kg),량자열량등치,지후매천주사1차,련속10d.검측A조대서상후즉각급B、C조상후1、4、7、10 d혈청TNF-α、폐조직균장거서세포염증단백1α(MIP-1α)수평,면역조직화학염색법관찰폐조직NF-KB p65급거서세포유주억제인자(MIF)표체정황.대실험수거진행t검험.결과 상후소유시상점3조대서각항검측지표비교,균위B、C조명현고우A조,t치위3.411～8.782,P치균소우0.01.상후4、7、10 d,B조각항지표수평명현저우C조,t치위2.321～2.785,P치균소우0.05.A조TNF-α위(0.96±0.32) ng/mL、MIP-1α위(76±16) pg/mL、NF-kB p65위(0.24±0.03)분、MIF위(1.31±0.03)분；B조이상지표상후7d체봉치,분별위(2.43±0 32) ng/mL、(210±56) pg/mL、(4.23±2.15)분、(4.69±1.83)분；C조이상지표상후7d위(3.15±0 54) ng/mL、(274±64) pg/mL、(5.15±2.31)분、(5.37±2.16)분.결론 ω-3 PUFA능강저탕상대서혈청TNF-α함량화폐조직MIP-1α、NF-KB p65、MIF수평,대폐조직유일정보호작용.
Objective To study the effect of ω-3 polyunsaturated fatty acids (ω-3 PUFA) on inflammation in lung tissue of rats with severe scald and its mechanism.Methods Seventy-two adult SD rats were divided into sham scald group (SS,n =8),treatment group 1 (T1,n =32),treatment group 2 (T2,n =32) according to the random number table.Rats in T1 group and T2 group were inflicted with 30％ TBSA full-thickness scald,and then they were respectively injected with 100 g/L ω-3 PUFA ( 1 mL/kg) and 200 g/L long-chain fatty acid (2 mL/kg) via tail vein within 5 minutes after burn.The above two fatty acids with equivalent calories were continuously injected for 10 days ( once a day).On post burn day (PBD) 1,4,7,and 10,serum level of TNF-α and level of macrophage inflammatory protein 1α (MIP-1α) in lung homogenate of T1 and T2 groups were detected,the levels of NF-kBp65 and macrophage migration inhibitory factor (MIF) in lung tissue of T1 and T2 groups were observed with immunohistochemical staining (recorded as score).Above-mentioned parameters were also determined in SS group.Data were processed with t test.Results The levels of 4 parameters in T1 and T2 groups on PBD 1,4,7,10 were higher than those in SS group (with t values from 3.411 to 8.782,P values all below 0.01 ),and those in T1 group on PBD 4,7,10 were lower than those in T2 group (with t values from 2.321 to 2.785,P values all below 0.05).The serum level of TNF-α and levels of MIP-1α,NF-kBp65,and MIF in lung tissue in SS group was respectively (0.96 ± 0.32) ng/mL,(76 ± 16 ) pg/mL,0.24 ± 0.03,1.31 ± 0.03,and those in T1 and T2 groups all peaked on PBD 7 [ (2.43 ± 0.32) ng/mL,(210 ± 56) pg/mL,4.23 ± 2.15,4.69 ±1.83; (3.15±0.54) ng/mL,(274±64) pg/mL,5.15±2.31,5.37±2.16].Conclusions ω-3 PUFA can effectively reduce serum level of TNF-α and levels of MIP-1α,NF-kBp65,and MIF in lung tissue of rats with severe scald,showing that it has a protective effect against injury of lung tissue.
