CAJ | 학술논문

目的探讨异基因造血干细胞移植联合不同剂量内皮祖细胞(EPC)输注对移植后造血重建的影响.方法以C57BL/6小鼠为供鼠,Balb/c小鼠为受鼠,进行HSCT,输注骨髓单个核细胞数量为5×106个/只.仅进行HSCT者为单纯骨髓细胞移植组；行HSCT的同时经尾静脉输注供者骨髓单个核细胞诱导培养的EPC的受鼠为EPC联合移植组,EPC的输注量分别为5×104、1×105、5× 105和1×106个/只.另设正常对照组和致死量照射组.观察小鼠的存活率、造血重建情况及骨髓微环境的变化.结果各EPC联合移植组小鼠存活时间长于单纯骨髓移植组,5×105 EPC联合移植组至观察结束时存活率为100％,高于其他各组(P＜0.05).移植后10和15 d,5×105 EPC联合移植组外周血白细胞数量高于其他组(P＜0.05).移植后15 d,5×105 EPC联合移植组外周血血小板数量高于其他组(P＜0.05).5×105 EPC联合移植组造血组织增生程度也好于其他组.5×105 EPC联合移植组骨髓内HSC比例为(1.06±0.03)％,高于其他各组(P＜0.05).结论小鼠异基因骨髓移植中联合输注5×105 EPC能够有效促进造血重建,提高小鼠存活率.
목적 탐토이기인조혈간세포이식연합불동제량내피조세포(EPC)수주대이식후조혈중건적영향.방법 이C57BL/6소서위공서,Balb/c소서위수서,진행HSCT,수주골수단개핵세포수량위5×106개/지.부진행HSCT자위단순골수세포이식조；행HSCT적동시경미정맥수주공자골수단개핵세포유도배양적EPC적수서위EPC연합이식조,EPC적수주량분별위5×104、1×105、5× 105화1×106개/지.령설정상대조조화치사량조사조.관찰소서적존활솔、조혈중건정황급골수미배경적변화.결과 각EPC연합이식조소서존활시간장우단순골수이식조,5×105 EPC연합이식조지관찰결속시존활솔위100％,고우기타각조(P＜0.05).이식후10화15 d,5×105 EPC연합이식조외주혈백세포수량고우기타조(P＜0.05).이식후15 d,5×105 EPC연합이식조외주혈혈소판수량고우기타조(P＜0.05).5×105 EPC연합이식조조혈조직증생정도야호우기타조.5×105 EPC연합이식조골수내HSC비례위(1.06±0.03)％,고우기타각조(P＜0.05).결론 소서이기인골수이식중연합수주5×105 EPC능구유효촉진조혈중건,제고소서존활솔.
Objective To explore a proper dose of endothelial progenitor cells (EPCs)administration that can achieve optimal hematopoietic improving effectiveness in a murine allogeneic hernatopoietic stem cell transplantation (allo-HSCT) model.Methods Female Balb/c mice were lethally irradiated with 60Co source,and then were injected intravenously with 5 106 bone marrow cells from C57BL/6 mice (bone marrow transplantation group).In co-transfer experiments,5 × 104,1 ×105,5 × 105 or 1 × 106 donor EPCs (EPCs treated groups) were injected simultaneously with bone marrow cells.The recipients were monitored for survival,peripheral white blood cells,hematopoietic stem cells (HSCs) and bone marrow histology.Results Compared with bone marrow transplantation group,all EPCs treated groups had accelerated recovery of peripheral white blood cells (P＜0.05),platelets (P＜0.05) and HSCs (P＜0.05).When infused with less than 5 × 105 EPCs,these effective hernatopoietic improving phenomena showed a positive correlation with the administrated doses of EPCs.However,when infused with 1 × 106 EPCs,the mice showed lower survival rate (P＜0.05)and slower recovery of peripheral white blood cells (P＜0.05),platelets (P＜0.05) and HSCs (P＜0.05) than 5 × 105 EPCs treated grpup.Bone marrow histopathology analysis confirmed the above findings.Conclusion Co-transfer with donor EPCs can improve survival rate and hematopoietic reconstitution of recipient mice in allo-HSCT,and 5 × 105 EPCs should be a proper dose to achieve the best effectiveness.