CAJ | 학술논문

目的对2008年分离自辽宁蚊虫的乙脑病毒株进行全基因组序列测定和分析,了解其全基因组特征.方法使用针对乙脑病毒全基因组测序引物,RT-PCR扩增片段,完成对病毒全基因组序列的测定.应用Chstal X(1.83)、ATGC(V4)、DNAStar、GENEDOC(3.2)、Mega(4.0)等生物学软件完成全基因组核苷酸和氨基酸序列分析及病毒的系统进化分析.结果乙脑病毒LN0828株基因组全长10 965个核苷酸,其中从97位到10 392位为开放读码框,编码3432个氨基酸.与GenBank中的32株乙脑病毒在全基因组水平的核苷酸总体差异率为1.6%～16.4%,氨基酸总体差异率为0.3%～5.1%.与减毒活疫苗株SA14-14-2相比,编码区共存在1186个核苷酸差异,86个氨基酸差异.全基因组序列系统进化分析显示LN0828株属于基因Ⅰ型乙脑病毒.结论与该地区2002年和2007年乙脑病毒分离株高度同源,关键位点氨基酸未见变异.
목적 대2008년분리자료녕문충적을뇌병독주진행전기인조서렬측정화분석,료해기전기인조특정.방법 사용침대을뇌병독전기인조측서인물,RT-PCR확증편단,완성대병독전기인조서렬적측정.응용Chstal X(1.83)、ATGC(V4)、DNAStar、GENEDOC(3.2)、Mega(4.0)등생물학연건완성전기인조핵감산화안기산서렬분석급병독적계통진화분석.결과 을뇌병독LN0828주기인조전장10 965개핵감산,기중종97위도10 392위위개방독마광,편마3432개안기산.여GenBank중적32주을뇌병독재전기인조수평적핵감산총체차이솔위1.6%～16.4%,안기산총체차이솔위0.3%～5.1%.여감독활역묘주SA14-14-2상비,편마구공존재1186개핵감산차이,86개안기산차이.전기인조서렬계통진화분석현시LN0828주속우기인Ⅰ형을뇌병독.결론 여해지구2002년화2007년을뇌병독분리주고도동원,관건위점안기산미견변이.
Objective To sequence and analyze the whole genome of Japanese encephalitis virus (JEV) isolated from mosquitoes in Liaoning province in 2008. Methods Using RT-PCR to amplify fragments with genome sequencing primer. The full-length genome was obtained by sequencing and sphcing. The differentiation analysis for nucleotides, deduced amino acid sequence and phylogenetic tree was performed by the software of Clustal X (1.83), ATGC(V4), DNAStar, GENEDOC(3.2) and Mega(4.0). Results The whole genome of strain LN0828 possesses 10 965 nucleotides. An open reading frame from 97 to 10 392 including 10 296 nucleotides is capable of coding for a 3432 amino acid polyprotein. Comparison of strain LN0828 genomic sequence with those of 32 JEV isolates in GenBank showed that nacleotide sequence divergence ranges from 1.6% to 16.4%, which resulted in amino acid sequence divergence from 0.3% to 5.1%. In comparison with live attenuated vaccine stain SA14-14-2 in open reading frame, strain LN0828 has a total of 1186 nucleotide substitutions, 86 amino acid divergences. Based on phylogenetic analysis, the strain LN0828 belongs to the genotype 1 JEV. Conclusion The whole genome of strain LN0828 is close tu those of isolates from Liaoning in 2002 and 2007, which were grouped into genotype I JEV.