中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2008年
11期
733-736
,共4页
王珺%邵宗鸿%付蓉%阮二宝%瞿文%梁勇%刘鸿%吴玉红%宋嘉%王化泉%邢莉民%关晶%李丽娟%刘惠%董舒文%由莉%邹鹏
王珺%邵宗鴻%付蓉%阮二寶%瞿文%樑勇%劉鴻%吳玉紅%宋嘉%王化泉%邢莉民%關晶%李麗娟%劉惠%董舒文%由莉%鄒鵬
왕군%소종홍%부용%원이보%구문%량용%류홍%오옥홍%송가%왕화천%형리민%관정%리려연%류혜%동서문%유리%추붕
贫血,再生障碍性%树突细胞%转录因子,T-bet%转录因子,GATA-3
貧血,再生障礙性%樹突細胞%轉錄因子,T-bet%轉錄因子,GATA-3
빈혈,재생장애성%수돌세포%전록인자,T-bet%전록인자,GATA-3
Anemia,aplastic%Dendritic cell%Transcriptive factor,T-bet%Transcriptive fac-tor,GATA-3
目的 探讨树突细胞(DC)亚群和淋巴细胞转录因子T-bet、GATA-3表达在获得性重型再生障碍性贫血(SAA)患者免疫失衡中的意义.方法 采用流式细胞术检测29例SAA患者及16名健康人外周血DCI(HLA-DR+Lin-CD11c+)、DC2(HLA-DR+Lin-CD123+)数值及比例,半定量RT-PCR检测外周血T-bet mRNA和GATA-3 mRNA表达,ELISA法检测血浆IFNγ、IL-4水平.结果 SAA初治组、恢复组DC1占外周血单个核细胞比例分别为(0.44±0.24)%、(0.73±0.30)%,明显高于正常对照组[(0.29±0.10)%,P<0.05].三组DC2分别为(0.18±0.14)%、(0.28±0.20)%、(0.29±0.13)%,初治组低于对照组(P<0.05).SAA初治组、恢复组DC1/DC2比值分别为3.45±2.71、2.90±0.95,明显高于对照组(1.15±0.56),初治组与恢复组DC1/DC2比值差异无统计学意义(P>0.05).SAA初治组、恢复组及对照组T-bet的相对表达量分别为0.37±0.07、0.20±0.07、0.17±0.05,初治组明显高于恢复组和对照组(P<0.05).三组中GATA-3的相对表达量差异无统计学意义(P>0.05).初治组T-bet/GATA-3比值为0.72±0.13,明显高于恢复组(0.33±0.08)和对照组(0.35±0.11).初治组血浆IFNγ/为(50.9±1.1)ng/L,明显高于恢复组[(49.7±0.9)ng/L]和正常对照组[(49.7±0.7)ng/L].T-bet表达与DC1/DC2比值、IFNγ水平呈显著正相关(r=0.445和r=0.402,P<0.01).结论 DC1/DC2和T-bet/GATA-3比值失衡可作为判断SAA患者免疫状态的指标.T-bet的相对表达量与SAA患者的病情有关,病情好转后T-bet/GATA-3恢复平衡.SAA患者DC亚群恢复迟于血常规恢复,因此不可过早停用免疫抑制剂.
目的 探討樹突細胞(DC)亞群和淋巴細胞轉錄因子T-bet、GATA-3錶達在穫得性重型再生障礙性貧血(SAA)患者免疫失衡中的意義.方法 採用流式細胞術檢測29例SAA患者及16名健康人外週血DCI(HLA-DR+Lin-CD11c+)、DC2(HLA-DR+Lin-CD123+)數值及比例,半定量RT-PCR檢測外週血T-bet mRNA和GATA-3 mRNA錶達,ELISA法檢測血漿IFNγ、IL-4水平.結果 SAA初治組、恢複組DC1佔外週血單箇覈細胞比例分彆為(0.44±0.24)%、(0.73±0.30)%,明顯高于正常對照組[(0.29±0.10)%,P<0.05].三組DC2分彆為(0.18±0.14)%、(0.28±0.20)%、(0.29±0.13)%,初治組低于對照組(P<0.05).SAA初治組、恢複組DC1/DC2比值分彆為3.45±2.71、2.90±0.95,明顯高于對照組(1.15±0.56),初治組與恢複組DC1/DC2比值差異無統計學意義(P>0.05).SAA初治組、恢複組及對照組T-bet的相對錶達量分彆為0.37±0.07、0.20±0.07、0.17±0.05,初治組明顯高于恢複組和對照組(P<0.05).三組中GATA-3的相對錶達量差異無統計學意義(P>0.05).初治組T-bet/GATA-3比值為0.72±0.13,明顯高于恢複組(0.33±0.08)和對照組(0.35±0.11).初治組血漿IFNγ/為(50.9±1.1)ng/L,明顯高于恢複組[(49.7±0.9)ng/L]和正常對照組[(49.7±0.7)ng/L].T-bet錶達與DC1/DC2比值、IFNγ水平呈顯著正相關(r=0.445和r=0.402,P<0.01).結論 DC1/DC2和T-bet/GATA-3比值失衡可作為判斷SAA患者免疫狀態的指標.T-bet的相對錶達量與SAA患者的病情有關,病情好轉後T-bet/GATA-3恢複平衡.SAA患者DC亞群恢複遲于血常規恢複,因此不可過早停用免疫抑製劑.
목적 탐토수돌세포(DC)아군화림파세포전록인자T-bet、GATA-3표체재획득성중형재생장애성빈혈(SAA)환자면역실형중적의의.방법 채용류식세포술검측29례SAA환자급16명건강인외주혈DCI(HLA-DR+Lin-CD11c+)、DC2(HLA-DR+Lin-CD123+)수치급비례,반정량RT-PCR검측외주혈T-bet mRNA화GATA-3 mRNA표체,ELISA법검측혈장IFNγ、IL-4수평.결과 SAA초치조、회복조DC1점외주혈단개핵세포비례분별위(0.44±0.24)%、(0.73±0.30)%,명현고우정상대조조[(0.29±0.10)%,P<0.05].삼조DC2분별위(0.18±0.14)%、(0.28±0.20)%、(0.29±0.13)%,초치조저우대조조(P<0.05).SAA초치조、회복조DC1/DC2비치분별위3.45±2.71、2.90±0.95,명현고우대조조(1.15±0.56),초치조여회복조DC1/DC2비치차이무통계학의의(P>0.05).SAA초치조、회복조급대조조T-bet적상대표체량분별위0.37±0.07、0.20±0.07、0.17±0.05,초치조명현고우회복조화대조조(P<0.05).삼조중GATA-3적상대표체량차이무통계학의의(P>0.05).초치조T-bet/GATA-3비치위0.72±0.13,명현고우회복조(0.33±0.08)화대조조(0.35±0.11).초치조혈장IFNγ/위(50.9±1.1)ng/L,명현고우회복조[(49.7±0.9)ng/L]화정상대조조[(49.7±0.7)ng/L].T-bet표체여DC1/DC2비치、IFNγ수평정현저정상관(r=0.445화r=0.402,P<0.01).결론 DC1/DC2화T-bet/GATA-3비치실형가작위판단SAA환자면역상태적지표.T-bet적상대표체량여SAA환자적병정유관,병정호전후T-bet/GATA-3회복평형.SAA환자DC아군회복지우혈상규회복,인차불가과조정용면역억제제.
Objective To investigate the relationship between the dendritic cell (DC) subsets and transcriptive factors, T-bet, GATA-3, and immune imbalance in acquired severe aplastic anemia (SAA). Methods The DCI (HLA-DR+ Lin- CD11c+) and DC2(HLA-DR+ Lin- CD123+) in peripheral blood mononuchar cells (PBMNC) were measured with flow cytometry (FCM), the expressions of T-bet mRNA and GATA-3 mRNA in PBMNC with semiquantitative RT-PCR and the plasma level of IFNγ and IL-4 with ELISA in 29 SAA patients and 16 healthy controls. Results ①The percentages of DC1 in PBMNC were (0.44 ± 0.24)% and (0.73 ± 0.30)% in untreated and recovered SAA patients respectively, both were higher than that in controls (0.29 ± 0.10) % (P < 0.05). The percentage of DC2 in the untreated cases was lower than that of recovered ones or controls [(0.18 ± 0.14) % vs (0.28 ± 0.20) % and (0.29 ± 0.13) %] (P < 0.05). DC1/DC2 ratios were 3.45 ± 2.71 and 2.90 ± 0.95 in untreated and recovered groups respectively, both were higher than that in controls (1.15 ± 0.56) (P < 0.05). No statistic difference in DC1/DC2 ratio was found between untreated and recovered patients (P <0.05). ② The relative mRNA expression levels of tranacriptive factor T-bet were 0.37±0.07, 0.20±0.07 and 0.17±0.05 in the above 3 groups, respectively, untreated group being higher than that of recovered group or healthy controls (P < 0.05). There was no statistic difference of GATA-3 expression among the 3 groups (P > 0.05). T-bet/ GATA-3 ratio was 0.72±0.13 in untreated group, being higher than that of recovered group (0.33±0.08) or controls (0.35 ± 0.11). ③ The plasma level of IFNγ in the untreated group was (50.9 ± 1.1) ng/L, which was higher than that of recovered group [(49.7±0.9) ng/L] or controls[(49.7±0.7) ng/L]. ④There was significant positive correlations between T-bet and DC1/DC2 ratio (r = 0.445, P < 0.01), as well as between T-bet and IFNγ (r = 0.402, P < 0.01). Conclusion Either DC1/DC2 or T-bet/GATA-3 ratio might become an index to estimate immune imbalance. High-expressed T-bet was related to the progress of SAA. In patients with SAA, DC1/DC2 ratio returns to normal range later than that of routine blood test does, indicating that immunosuppressive therapy should not be withdrawn toe earlier.
