CAJ | 학술논문

本试验对100只3日龄雏鸡用人工口服感染Ⅷ型腺病毒建立了鸡包涵体肝炎（Inclusion body hepytitis-IBH）模型后以免疫金银法进行免疫组织化学动态研究。结果表明，该病毒对鸡的肝脏、胸腺、法氏囊、脾脏、小肠、肾脏、胰腺等脏器的上皮细胞、淋巴细胞、血管内皮细胞，窦内皮细胞及红细胞等均具有亲嗜性，以肝脏、法氏囊、肾脏最为明显。此外，多数组织在接种12 h～2 d及9 d～11 d细胞核内两次出现胶体金颗粒，其形态表现为粗大颗粒及微细颗粒两种。
본시험대100지3일령추계용인공구복감염Ⅷ형선병독건립료계포함체간염（Inclusion body hepytitis-IBH）모형후이면역금은법진행면역조직화학동태연구。결과표명，해병독대계적간장、흉선、법씨낭、비장、소장、신장、이선등장기적상피세포、림파세포、혈관내피세포，두내피세포급홍세포등균구유친기성，이간장、법씨낭、신장최위명현。차외，다수조직재접충12 h～2 d급9 d～11 d세포핵내량차출현효체금과립，기형태표현위조대과립급미세과립량충。
Immunohistochemical dynamic changes of inclusion body hepatitis （IBH） were studied following the oral administration of a serotype 8 strain of avian adenovirus into 3-day-old chickens．The results showed that the adenovirus had an affinity to the epithelia，lymphocytes，the blood vessel and sinus endothelium and erythrocytes in the livers，thymuses，bursas，spleens，intestines，kidneys and pancreases．In addition，during 12 h-2days，9 days-11 days post inoculation（pi），the nuclei of most organs were positive twice．The positive particles in the nuclei have two kinds of appearances：large and fine particular．