CAJ | 학술논문

目的研究染料木素和槲皮素对体外肝纤维化的保护作用.方法细胞增殖和胶原合成分别用结晶紫染色法和3H-脯氨酸参入法.原胶原mRNA水平用RT-PCR法测定.结果染料木素(25～70μmo1@L-1)和槲皮素(6.25～50μmo1@L-1)浓度依赖抑制PDGF促HSC-T6细胞增殖和TGFβ1刺激的胶原合成及I型原胶原mRNA的表达.结论染料木素和槲皮素抑制PDGF和TGFβ1对星状细胞的作用可能对肝纤维化有保护作用.
목적연구염료목소화곡피소대체외간섬유화적보호작용.방법세포증식화효원합성분별용결정자염색법화3H-포안산삼입법.원효원mRNA수평용RT-PCR법측정.결과염료목소(25～70μmo1@L-1)화곡피소(6.25～50μmo1@L-1)농도의뢰억제PDGF촉HSC-T6세포증식화TGFβ1자격적효원합성급I형원효원mRNA적표체.결론염료목소화곡피소억제PDGF화TGFβ1대성상세포적작용가능대간섬유화유보호작용.
Objective: To study the antifibrotic effects of genistein(GE) and quercetin(QU) on rat hepatic stellate HSC-T6 cell proliferation stimulated with platelet-derived growth factor (PDGF), collagen synthesis and type I procollagen messenger RNA (mRNA) expression stimulated with transforming growth factor β1 (TGFβ1). Methods:Cell proliferation was measured by crystal violet staining assay. Collagen synthesis was determined by 3H-proline incorporation assay. Type I procollagen mRNA level was determined by reverse transcription polymerase chain reaction (RT-PCR). Results: GE (25～70 μmol@L-1) and QU (6.25～50 μmol@ L-1) concentration-dependently attenuated PDGF-driven HSC-T6 cell proliferative activity. TGFβ1-stimulated collagen synthesis was also reduced. This was associated with a decrease in type I procollagen mRNA expression, indicating an effect at a pretranslational level.Conclusion: GE and QU may have therapeutic potential against liver fibrosis by regulating PDGF and TGFβ1actions.