CAJ | 학술논문

目的:探讨一氧化氮合酶在豚鼠耳蜗内的分布以及对毛细血管张力的作用机制.方法:经4 g/L多聚甲醛心脏灌注固定后,取出耳蜗,经3 g/L依地酸脱钙后,作10 μm厚冰冻切片,用辅酶Ⅱ孵育液在37℃条件下孵育1 h.结果:发现在耳蜗内、外毛细胞底部与耳蜗神经末梢接头处及毛细血管球内皮细胞有明显NADPH-黄递酶活性,此外在内、外柱细胞、支持细胞、血管纹及螺旋神经节细胞也有NADPH-黄递酶活性反应.结论:一氧化氮(NO)在维持耳蜗正常神经传导及毛细血管张力中起着重要作用.
목적:탐토일양화담합매재돈서이와내적분포이급대모세혈관장력적작용궤제.방법:경4 g/L다취갑철심장관주고정후,취출이와,경3 g/L의지산탈개후,작10 μm후빙동절편,용보매Ⅱ부육액재37℃조건하부육1 h.결과:발현재이와내、외모세포저부여이와신경말소접두처급모세혈관구내피세포유명현NADPH-황체매활성,차외재내、외주세포、지지세포、혈관문급라선신경절세포야유NADPH-황체매활성반응.결론:일양화담(NO)재유지이와정상신경전도급모세혈관장력중기착중요작용.
AIM: To explore the effect and mechanism of nitric oxide synthase(NOS) in the regulation of nerve conduction and capillary blood flow in guinea pig cochlea.METHODS: After paraformaldehyde perfusion of the heart in 6 guinea pigs, the cochleas taken to prepared frozen sections following decalcification with edetic acid. The sections were incubated in nicotinamide adenine dinucleotide phosphate hydrogen(NADPH) for charactering the distribution and activity of NADPH-diaphorase by means of enzyme-linked immunosorbent assay(ELISA).RESULTS: Obvious NADPH-diaphorase activity was found in the guinea pig cochlea, cochlea nerve ending underlying the outer hair cells, and the endothelial cells of the capillary glomus. In addition, NADPH-diaphorase activity was also detected in the outer and inner pillar cells, supporting cells,spiral ligaments, and spiral ganglion cells.CONCLUSION: Nitric oxide is crucial in maintaining normal nerve conduction and capillary blood flow in the cochlea of guinea pigs.