CAJ | 학술논문

建立了一个胶束电动色谱-二极管阵列检测方法,用于分离测定山梨酸钾、苯甲酸钠和糖精钠.在优化条件下,使用24 mmol/L十二烷基硫酸钠、20 mmol/L硼砂,pH 9.6、电压12 kV,分离时间仅用4.2 min.测定范围内,峰高与分析物浓度呈良好的线性关系,迁移时间和峰高的相对标准偏差(n=6)小于0.63％,3个分析物的检测限分别为0.42、0.43和0.42 μg/L,与高效液相色谱方法相比灵敏度增加了约10～100倍.方法被用于实际饮料样品中的3种食品添加剂的测定,回收率89.3％～112.0％.
건립료일개효속전동색보-이겁관진렬검측방법,용우분리측정산리산갑、분갑산납화당정납.재우화조건하,사용24 mmol/L십이완기류산납、20 mmol/L붕사,pH 9.6、전압12 kV,분리시간부용4.2 min.측정범위내,봉고여분석물농도정량호적선성관계,천이시간화봉고적상대표준편차(n=6)소우0.63％,3개분석물적검측한분별위0.42、0.43화0.42 μg/L,여고효액상색보방법상비령민도증가료약10～100배.방법피용우실제음료양품중적3충식품첨가제적측정,회수솔89.3％～112.0％.
A new micellar electrokinetic chromatography with photo diode array detection method was developed to separate and determine potassium sorbate,sodium benzoate and sodium saccharin.In conditions of sodium dodecyl sulphate 24 mmol/L,Na2B4O7 20 mmol/L,pH 9.6,and separation voltage 12 kV,the separation was achieved within 4.2 min.Good linear relationships between peak height and concentration were found in the tested range.Relative standard deviations (n=6) of migration times or peak heights were less than 0.63％.The detection limits for potassium sorbate,sodium benzoate and sodium saccharin were 0.42,0.43,and 0.42 μg/L respectively.The sensitivity was improved ten to a hundred times more than those of high performance liquid chromatography method reported recently.The method was applied to the determination of the three food additives in real drink samples with recoveries of 89.3％-112.0％.