CAJ | 학술논문

目的探讨腺样囊性癌(adenoid cystic carcinoma,ACC)组织中神经生长因子(nerve growth factor,TGF)及上皮钙黏素(E-cadherin,E-cad)、钙结合蛋白A4(S100A4)的表达和临床意义,分析它们之间的相关性及其与ACC嗜神经侵袭(perineural invasion,PNI)现象的关系,进一步探讨NGF在PNI过程中对ACC细胞的黏附力和迁移运动能力的影响.方法对42例ACC组织按年龄、性别、发病部位、临床分期及PNI和非PNI(NPNI)进行分组,42例中PNI组26例,NPNI组16例,应用免疫组化SP法检测其中NGF、E-cad和S100A4的表达与分布,分析三者与临床病理参数之间的关系,并对它们在不同病理类型和组织学部位的表达进行统计学分析.结果 NGF和S100A4在PNI组的表达率分别为88%(23/26)和77%(20/26),较NPNI组(8/16和7/16)高,差异有统计学意义(P＜0.05),两者在PNI组的表达呈正相关(r=0.316,P＜0.05).PNI组中E-cad的表达为31%(8/26),较NPNI组(12/16)低(P＜0.05),与NGF的表达(r=-0.385,P＜0.05)和S100A4的表达呈明显负相关(r=-0.612,P=0.000).NGF在PNI组ACC神经周组织的表达[83%(25/30)]高于远离神经部位[47%(14/30)],差异有统计学意义(P＜0.05).结论在ACC嗜神经侵袭过程中,NGF可能扮演着重要角色,但并非惟一因素,NGF可能通过和相应受体结合提高S100A4的表达率而降低E-cad 的表达和生物活性,使癌细胞间黏附力降低,运动能力增强进而促进ACC对神经组织的侵袭.
목적 탐토선양낭성암(adenoid cystic carcinoma,ACC)조직중신경생장인자(nerve growth factor,TGF)급상피개점소(E-cadherin,E-cad)、개결합단백A4(S100A4)적표체화림상의의,분석타문지간적상관성급기여ACC기신경침습(perineural invasion,PNI)현상적관계,진일보탐토NGF재PNI과정중대ACC세포적점부력화천이운동능력적영향.방법 대42례ACC조직안년령、성별、발병부위、림상분기급PNI화비PNI(NPNI)진행분조,42례중PNI조26례,NPNI조16례,응용면역조화SP법검측기중NGF、E-cad화S100A4적표체여분포,분석삼자여림상병리삼수지간적관계,병대타문재불동병리류형화조직학부위적표체진행통계학분석.결과 NGF화S100A4재PNI조적표체솔분별위88%(23/26)화77%(20/26),교NPNI조(8/16화7/16)고,차이유통계학의의(P＜0.05),량자재PNI조적표체정정상관(r=0.316,P＜0.05).PNI조중E-cad적표체위31%(8/26),교NPNI조(12/16)저(P＜0.05),여NGF적표체(r=-0.385,P＜0.05)화S100A4적표체정명현부상관(r=-0.612,P=0.000).NGF재PNI조ACC신경주조직적표체[83%(25/30)]고우원리신경부위[47%(14/30)],차이유통계학의의(P＜0.05).결론 재ACC기신경침습과정중,NGF가능분연착중요각색,단병비유일인소,NGF가능통과화상응수체결합제고S100A4적표체솔이강저E-cad 적표체화생물활성,사암세포간점부력강저,운동능력증강진이촉진ACC대신경조직적침습.
Objective To investigate the effect on adhesion and motion capbilities of adenoid cystic carcinoma(ACC) , by detecting the expression of nerve growth factor (NGF) , E-cadherin (E-cad) and S100A4 and the clinical significance in ACC tissues and analyzing the relationships between them with perineural invasion(PNI). Methods The expression of NGF, E-cad and S100A4 in ACC was detected with the way of immunohistochemistry SP, and then analyzing the expression level of them in different pathological types and histological regions in statistical ways on the basis of their relation with clinical and pathological parameters. Results The expression of NGF and S100A4 in PNI group [ 88% (23/26) and 77% (20/26) ] , was higher than that in NPNI group (8/16 and 7/16, P <0.05), and a positive correlation between them was identified in PNI group(r =0. 316,P <0.05). However the E-cad expression was lower in PNI group[31%(8/26) ,P<0. 05]. On the other hand it suggested a negative correlation with NGF (r = 0.385, P<0.05) as well as that with S100A4(r = -0.612,P =0.000). The expression level of NGF in fasciculus[ 83% (25/30)] has significant deviation compared with it in distant tumor tissues [ 47% (14/30), P<0. 05]. Conclusions In the PNI process of ACC, NGF plays important parts but not the only factor. It can increase the expression and activity of S100A4 but decrease E-cad expression through binding with its receptor. Thus, adhesion abilities of tumor cells was weakened and motional abilities was strengthen.