CAJ | 학술논문

目的探讨酒精致脑发育异常机制。方法从孕19 d鼠胚胎脑组织分离培养星形、少突胶质细胞体外分别施不同剂量酒精及其代谢产物乙醛，应用免疫细胞化学技术研究二者作用不同时间对星形、少突胶质细胞原癌基因 c-fos 表达影响。结果酒精对星形、少突胶质细胞c-fos表达与时间和剂量有关。各剂量酒精、乙醛作用1 h既可影响两种细胞c-fos 基因表达，2 h表达至峰值，72 h恢复正常呈现特异时相性。结论酒精、乙醛均可致星形、少突胶质细胞c-fos表达增强。c-fos异常表达很可能在酒精所致脑发育异常担当重要作用。
목적탐토주정치뇌발육이상궤제。방법종잉19 d서배태뇌조직분리배양성형、소돌효질세포체외분별시불동제량주정급기대사산물을철，응용면역세포화학기술연구이자작용불동시간대성형、소돌효질세포원암기인 c-fos 표체영향。결과주정대성형、소돌효질세포c-fos표체여시간화제량유관。각제량주정、을철작용1 h기가영향량충세포c-fos 기인표체，2 h표체지봉치，72 h회복정상정현특이시상성。결론주정、을철균가치성형、소돌효질세포c-fos표체증강。c-fos이상표체흔가능재주정소치뇌발육이상담당중요작용。
Objective　This paper is aimed to explore the mechanisms of brain development abnormality induced by alcohol.Methods　Astrocytes and oligodendrocytes of 19-day rat embryo were exploited and cultured in vitro，and alcohol and its metabolite product (acetaldehyde) were added to DMEMF12 medium.After different exposure times，c-fos expression of astrocytes and oligodendrocytes was measured by the immunocytochemistry technique.Results　Changes in c-fos gene expression induced by alcohol and acetaldehyde was time and dose dependent.After 1 hr exposure，alcohol and acetaldehyde affected c-fos gene expression in two kinds of neuralglia.C-fos positive expression reached peak value after 2 hr，but recovered after 72 hr and showed special time phase expression.Conclusions　Alcohol and acetaldehyde cause abnormal increase of c-fos gene expression in astrocytes and oligodendrocytes.This abnormal expression may play an important role in abnormal brain development induced by alcohol.