CAJ | 학술논문

目的了解河北正定县某镇自然人群感染乙型肝炎病毒(HBV)的"α"抗原决定簇突变情况,分析新生儿乙肝疫苗计划免疫对其的影响.方法为正定县某镇全体村民整群采集静脉血标本,用放射免疫法检测血清HBV-M,选取乙肝表面抗原(HBsAg)阳性者提取血清HBV DNA,扩增S基因,测序后与标准序列对比.结果 11 478份血清样品中,HBsAg检测阳性率4.78%.其中可用于HBV DNA提取的样品443份,扩增HBV S基因并成功测序434例.检测到该地区HBV"α"抗原决定簇氨基酸突变率6.7%,1986年前后出生人群HBV"α"抗原决定簇两个茎环的氨基酸突变率差异无统计学意义(P=1.0000,P=0.0877).结论该镇存在HBV"α"抗原决定簇突变株,其在乙肝疫苗免疫出生后人群中感染率较低,尚不能说明该镇新生儿乙肝疫苗接种会引发突变株的流行.
목적 료해하북정정현모진자연인군감염을형간염병독(HBV)적"α"항원결정족돌변정황,분석신생인을간역묘계화면역대기적영향.방법 위정정현모진전체촌민정군채집정맥혈표본,용방사면역법검측혈청HBV-M,선취을간표면항원(HBsAg)양성자제취혈청HBV DNA,확증S기인,측서후여표준서렬대비.결과 11 478빈혈청양품중,HBsAg검측양성솔4.78%.기중가용우HBV DNA제취적양품443빈,확증HBV S기인병성공측서434례.검측도해지구HBV"α"항원결정족안기산돌변솔6.7%,1986년전후출생인군HBV"α"항원결정족량개경배적안기산돌변솔차이무통계학의의(P=1.0000,P=0.0877).결론 해진존재HBV"α"항원결정족돌변주,기재을간역묘면역출생후인군중감염솔교저,상불능설명해진신생인을간역묘접충회인발돌변주적류행.
Objective To determine the" α" dominant mutation of hepatitis B virus(HBV) in community-based Zhengding. Analysis the role of the newborn hepatitis B vaccination on the mutation. Methods Based on the national surveillance of hepatitis B, 11 478 people's sera were collected and tested by SPRIA with kits. Collect people's sera with positive HBsAg and amplify the S gene. Sequencing and elastwaling them with the standard sequences. Results Overall, HBV DNA was successfully amplified and sequenced in 434 of 443 samples. 6.7 % samples mutated in HBV "α" dominant region. The difference between the mutation ratio of the two loops of HBV "α" dominant between the people born before and after the year 1986 has no significance. Conclusion There were HBV "α" dominant mutant virus in the local area with a low infection rate in the population born after the year 1986. It could not explain the newborn hepatitis B vaccination can induce the prevalence of the "α" dominant mutate HBV.