中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2009年
11期
992-996
,共5页
年华%耿文清%崔华露%张子宁%张旻%韩晓旭%赵彬%胡清海%尚红
年華%耿文清%崔華露%張子寧%張旻%韓曉旭%趙彬%鬍清海%尚紅
년화%경문청%최화로%장자저%장민%한효욱%조빈%호청해%상홍
HIV-1%单核细胞%TLR7%TLR8
HIV-1%單覈細胞%TLR7%TLR8
HIV-1%단핵세포%TLR7%TLR8
HIV-1%Monocyte%TLR7%TLR8
目的 对HIV-1感染者单核细胞TLR7/8表达水平及与疾病进展的相关性进行研究,探讨单核细胞TLR7/8在HIV-1疾病进程中的作用.方法 选取63名HIV-1感染者及18名健康对照,应用MACS磁珠分选法纯化CD14+单核细胞,用2.5μg/ml R848刺激单核细胞TLR7/8,QIAGEN公司的RNA提取试剂盒提取单核细胞总RNA,实时定量RT-PCR测定TLR7/8的mRNA表达.结果 HIV-1感染者单核细胞TLR7和TLR8的表达水平与CIM+T细胞显著正相关(r=0.614,P＜0.01;r=0.419,P＜0.01).TLR7 mRNA表达:缓慢进展组明显高于HIV感染组、AIDS组和健康对照组(P＜0.05),HIV感染组明显高于AIDS组(P＜0.05);TLR8 mRNA表达:缓慢进展组明显高于AIDS组(P＜0.05).体外用R848刺激11LR7后表达显著下调,而TLR8的表达稳定.结论 AIDS疾病进程中HIV-1感染者单核细胞TLR7/8表达水平明显下降,TLR7的表达水平下降更显著.
目的 對HIV-1感染者單覈細胞TLR7/8錶達水平及與疾病進展的相關性進行研究,探討單覈細胞TLR7/8在HIV-1疾病進程中的作用.方法 選取63名HIV-1感染者及18名健康對照,應用MACS磁珠分選法純化CD14+單覈細胞,用2.5μg/ml R848刺激單覈細胞TLR7/8,QIAGEN公司的RNA提取試劑盒提取單覈細胞總RNA,實時定量RT-PCR測定TLR7/8的mRNA錶達.結果 HIV-1感染者單覈細胞TLR7和TLR8的錶達水平與CIM+T細胞顯著正相關(r=0.614,P＜0.01;r=0.419,P＜0.01).TLR7 mRNA錶達:緩慢進展組明顯高于HIV感染組、AIDS組和健康對照組(P＜0.05),HIV感染組明顯高于AIDS組(P＜0.05);TLR8 mRNA錶達:緩慢進展組明顯高于AIDS組(P＜0.05).體外用R848刺激11LR7後錶達顯著下調,而TLR8的錶達穩定.結論 AIDS疾病進程中HIV-1感染者單覈細胞TLR7/8錶達水平明顯下降,TLR7的錶達水平下降更顯著.
목적 대HIV-1감염자단핵세포TLR7/8표체수평급여질병진전적상관성진행연구,탐토단핵세포TLR7/8재HIV-1질병진정중적작용.방법 선취63명HIV-1감염자급18명건강대조,응용MACS자주분선법순화CD14+단핵세포,용2.5μg/ml R848자격단핵세포TLR7/8,QIAGEN공사적RNA제취시제합제취단핵세포총RNA,실시정량RT-PCR측정TLR7/8적mRNA표체.결과 HIV-1감염자단핵세포TLR7화TLR8적표체수평여CIM+T세포현저정상관(r=0.614,P＜0.01;r=0.419,P＜0.01).TLR7 mRNA표체:완만진전조명현고우HIV감염조、AIDS조화건강대조조(P＜0.05),HIV감염조명현고우AIDS조(P＜0.05);TLR8 mRNA표체:완만진전조명현고우AIDS조(P＜0.05).체외용R848자격11LR7후표체현저하조,이TLR8적표체은정.결론 AIDS질병진정중HIV-1감염자단핵세포TLR7/8표체수평명현하강,TLR7적표체수평하강경현저.
Objective To examine the expression of TLR7/8 in monocytes purified from HIV-1 infected individuals and to study its association with disease progression. Methods Sixty-three HIV-1 infected individuals and 18 normal controls were enrolled. Monocytes were purified by MACS system and RNA of them was extracted by RNA mini kit of QIAGEN company. TLR7/8 expression was tested by real-time RT-PCR with ABI7500. Results It was found that the expression of TLR7 was strongly correlated with absolute CD4 count (r =0.614, P＜0.01) , so was TLR8 (r =0.419, P＜0.01). The expression of TLR7 in slow progressor (SP) group was higher than that in HIV-1 infected patients group, AIDS patients group and normal group (P ＜ 0. 05 ) . HIV group and normal group were strongly higher than AIDS group (P ＜ 0. 05). It was no significant differentiation of expression of TLR7 between HIV infection group and normal control group. The expression of TLR8 in SP group and normal group were significantly higher than that in AIDS group (P ＜ 0. 05). The expression of TLR8 was no singnificantly difference between SP group and HIV group or normal control group, so was it between HIV group or normal control group and AIDS group. Conclusion The expression of TLR7/8 in monocytes from HIV-1 infected patients significantly correlated with disease progression.
