CAJ | 학술논문

目的研究大鼠开放性骨折后T淋巴细胞的分子生物学改变.方法建立大鼠开放性骨折模型,采用流式细胞法记录检测T细胞亚群,用放免试剂盒测定cAMP,用普通生化法测定GSH(谷胱甘肽)、SOD(超氧化物歧化酶).结果与对照组相比CD3及CD4细胞百分比明显下降,CD8细胞百分比明显上升(P<0.01).cAMP、GSH、SOD均有明显变化.结论开放性骨折后由于T淋巴细胞的改变,机体处于免疫功能抑制状态.
목적연구대서개방성골절후T림파세포적분자생물학개변.방법건립대서개방성골절모형,채용류식세포법기록검측T세포아군,용방면시제합측정cAMP,용보통생화법측정GSH(곡광감태)、SOD(초양화물기화매).결과여대조조상비CD3급CD4세포백분비명현하강,CD8세포백분비명현상승(P<0.01).cAMP、GSH、SOD균유명현변화.결론개방성골절후유우T림파세포적개변,궤체처우면역공능억제상태.
Objective To study molecular biological changes of T lymphocyte after open fracture of rat.Methods Model of rat open fracture was constructed.T lymphocyte subsets were registered by using flowing-cell.cAMP was measured by using radio-immuny box.The concentration of GSH and SOD were determined by using common biochemical methods.Results Percentages of CD8 cells increased while those of CD3, CD4 cells reduced significantly as compared with the controls. So do cAMP, GSH, SOD. Conclusion For those changes of T lymphocyte after open fracture, the immunologic function in rat is depressed.