CAJ | 학술논문

目的:通过变应性鼻炎动物模型了解Eotaxin在变应性鼻炎中的表达以及变态反应炎性递质组胺对Eotaxin表达的影响,以期深入了解变应性鼻炎的发病机制.方法:卵蛋白致敏法建立变应性鼻炎大鼠模型,应用免疫组织化学、RT-PCR及ELISA法,观察组胺及组胺HR1拮抗剂干预后鼻黏膜、血清及鼻腔灌洗液中Eotaxin的表达量或含量变化.结果:变应性鼻炎干预组与变应性鼻炎无干预组相比,鼻腔灌洗液以及鼻黏膜中Eotaxin的表达量较前者显著增加(P＜0.05),而H1拮抗剂干预组血清,鼻腔灌洗液以及鼻黏膜中Eotaxin增加均显著受到抑制(P＜0.05).结论:本实验进一步确认了Eotaxin参与了变应性鼻炎发病的理论基础.组胺作为一种变应性鼻炎中的重要炎症递质可以通过影响Eotaxin的表达参与变应性鼻炎的发病过程,且HR1亦参与了这一过程.
목적:통과변응성비염동물모형료해Eotaxin재변응성비염중적표체이급변태반응염성체질조알대Eotaxin표체적영향,이기심입료해변응성비염적발병궤제.방법:란단백치민법건립변응성비염대서모형,응용면역조직화학、RT-PCR급ELISA법,관찰조알급조알HR1길항제간예후비점막、혈청급비강관세액중Eotaxin적표체량혹함량변화.결과:변응성비염간예조여변응성비염무간예조상비,비강관세액이급비점막중Eotaxin적표체량교전자현저증가(P＜0.05),이H1길항제간예조혈청,비강관세액이급비점막중Eotaxin증가균현저수도억제(P＜0.05).결론:본실험진일보학인료Eotaxin삼여료변응성비염발병적이론기출.조알작위일충변응성비염중적중요염증체질가이통과영향Eotaxin적표체삼여변응성비염적발병과정,차HR1역삼여료저일과정.
Objective: To explore the expression of Eotaxin and the effect of histamine in allergic rhinitis model (AR),and aim to explore the pathogenesis of AR. Method:The AR models were established by applicating of ovain albumin in rats. The expression of Eotaxin in nosal mucosa,serum and nasal cavity lavage fluid,were observed before and after treatment of histamine or its antagonist by immunochemistry,RT-PCR and ELISA technique. Result:The expression of Eotaxin in nasal lavage fluid and nasal mueosa increased after treatment of histamine(P＜0.05). Contrarily,the expression of Eotaxin in nasal lavage fluid,nasal mucosa and serum decreased after treatment of the antagonist of histamine. Conclusion:Both histamine and its receptor can involve in the pathogenesis of AR by affecting the expression of Eotaxin.