CAJ | 학술논문

背景:以往关于口腔综合治疗台水路细菌污染状况的研究较多,但口腔专科医院不同科室间因业务内容不同,其对口腔综合治疗台高速涡轮机水路的影响如何尚未见报道.目的:评估口腔专科医院不同专业各科室口腔综合治疗台高速涡轮机水路的细菌污染状况,以期为各科室制定个性化的感染控制措施提供依据.设计、时间及地点:横断面调查,于2007-05/2008-03完成实验及数据整理,取样于广东省口腔医院,细菌学检测于南方医科大学公共卫生与热带医学学院微生物实验室完成.材料:普通营养琼脂、血平板、微生物生化反应鉴定系统、链球菌凝集试验试剂盒、革兰染色液等购自广东环凯微生物试剂公司.方法:广东省口腔医院80张口腔综合治疗台接无菌高速涡轮机后取水样,取样时间为2007-05/10隔周星期五下班后,每机每次取6 mL,共取10次.采用常规微生物分离鉴定技术进行细菌学检测.主要观测指标:水样中细菌浓度和葡萄球菌、链球菌、放线菌3种细菌的检出率.结果:口腔医院各临床科室口腔综合治疗台高速涡轮机水路水样中细菌浓度5.15×10~1.59×10~3 cfu/mL,平均5.67×10~2 cfu/mL,牙周病科水样细菌浓度最高,口腔颌面外科水样细菌浓度最低,各科室间差异有显著性意义(P=0.000);各科室间水样3种细菌的检出率差异亦有显著性意义(p=0.000).结论:口腔专科医院不同科室的口腔综合治疗台高速涡轮机水路细菌污染状况不同,对于高速涡轮机使用频率较低的科室建议将空踩口腔综合治疗台高速涡轮机水路放水列入每天工作常规,而对于临床业务污染较严重的科室还应考虑采用额外的感染控制措施.
배경:이왕관우구강종합치료태수로세균오염상황적연구교다,단구강전과의원불동과실간인업무내용불동,기대구강종합치료태고속와륜궤수로적영향여하상미견보도.목적:평고구강전과의원불동전업각과실구강종합치료태고속와륜궤수로적세균오염상황,이기위각과실제정개성화적감염공제조시제공의거.설계、시간급지점:횡단면조사,우2007-05/2008-03완성실험급수거정리,취양우광동성구강의원,세균학검측우남방의과대학공공위생여열대의학학원미생물실험실완성.재료:보통영양경지、혈평판、미생물생화반응감정계통、련구균응집시험시제합、혁란염색액등구자엄동배개미생물시제공사.방법:광동성구강의원80장구강종합치료태접무균고속와륜궤후취수양,취양시간위2007-05/10격주성기오하반후,매궤매차취6 mL,공취10차.채용상규미생물분리감정기술진행세균학검측.주요관측지표:수양중세균농도화포도구균、련구균、방선균3충세균적검출솔.결과:구강의원각림상과실구강종합치료태고속와륜궤수로수양중세균농도5.15×10~1.59×10~3 cfu/mL,평균5.67×10~2 cfu/mL,아주병과수양세균농도최고,구강합면외과수양세균농도최저,각과실간차이유현저성의의(P=0.000);각과실간수양3충세균적검출솔차이역유현저성의의(p=0.000).결론:구강전과의원불동과실적구강종합치료태고속와륜궤수로세균오염상황불동,대우고속와륜궤사용빈솔교저적과실건의장공채구강종합치료태고속와륜궤수로방수렬입매천공작상규,이대우림상업무오염교엄중적과실환응고필채용액외적감염공제조시.
BACKGROUND: The presence of various microorganisms in dental unit waterlines has been revealed by numerous reports previously. However, impact of different dental procedures on microbial contamination in dental unit waterlines (DUWLs) remains unclearly.OBJECTIVE: To assess the level of microbial contamination in water effluent from the high-speed handpiece line of dental chair units (DCUs) among different specialty departments in a provincial stomatological hospital. Thus, to propose individual infection control measures for different specialty departments according to their properties of microbial contamination.DESIGN, TIME AND SETTING: A cross-sectional investigation was designed and conducted in Guangdong Provincial Stomatological Hospital and Laboratory of Microbiology, School of Public Health and Tropical Medicine, Southern Medical University, between May 2007 and March 2008.MATERIALS: The materials used in this investigation including nutrient agar, blood plates, biochemical testing kit, agglutination testing kit and Gram staining agents. These materials were purchased from Guangdong Huankai Microbial Science and Technology Co., Ltd.METHODS: Water sampling was conducted at 80 DCUs of different specialty departments in Guangdong Provincial Stomatological Hospital. A total of 60 mL, with 6 mL for once sampling of water effluent from the high-speed handpiece line of each DCU were collected aseptically after the finish of the daily clinical work on every second Friday between May and October in 2007. Standard isolation and identification technique of bacteria was adopted.MAIN OUTCOME MEASURES: Concentration of bacteria and prevalence of 3 species of bacteria in water effluent from the high-speed handpiece line of DCUs among different specialty departments were evaluated.RESULTS: Concentration of bacteria in water effluent from the high-speed handpiece line of DCUs averaged 5.67×10~2 cfu/mL, ranged from 5.15×10 to 1.59×10~3 cfu/mL. The microbial concentration of water samples from Department of Periodontics was significantly higher than that from other departments, while the microbial concentration of water samples from Department of Oral Surgery was the lowest (P = 0.000).CONCLUSION: Contamination in water effluent from the high-speed handpiece line of DCUs varied from different specialty departments in the provincial stomatological hospital. It is suggested that DUWLs flushing should be performed routinely, especially for the department without frequent use of the high-speed handpiece in the daily work, and extra infection control measures should be adopted for the department with severe clinical operative contamination.