CAJ | 학술논문

研究两种从盘花垂头菊中分离出的高含氧甜没药烯型倍半萜(HOBS)化合物对肿瘤细胞的影响.结果显示HOBS能够诱导人肝癌细胞SMMC-7721凋亡.单细胞电泳实验证明HOBS能够以剂量依赖的方式诱导SMMC-7721细胞损伤,流式细胞技术显示HOBS能够以剂量依赖的方式诱导SMMC-7721细胞凋亡,凋亡率达到38.9%,ci(Ca2+)的测定显示HOBS能够诱导ci(Ca2+)升高,所有的结果证明HOBS能够诱导人肝癌细胞SMMC-7721凋亡.提示这两种化合物有抗人肝癌细胞的SMMC-7721活性,为抗肿瘤药物的设计提供了新的思路.
연구량충종반화수두국중분리출적고함양첨몰약희형배반첩(HOBS)화합물대종류세포적영향.결과현시HOBS능구유도인간암세포SMMC-7721조망.단세포전영실험증명HOBS능구이제량의뢰적방식유도SMMC-7721세포손상,류식세포기술현시HOBS능구이제량의뢰적방식유도SMMC-7721세포조망,조망솔체도38.9%,ci(Ca2+)적측정현시HOBS능구유도ci(Ca2+)승고,소유적결과증명HOBS능구유도인간암세포SMMC-7721조망.제시저량충화합물유항인간암세포적SMMC-7721활성,위항종류약물적설계제공료신적사로.
The effects of two novel highly oxygenated bisabolane-type sesquiterpenes (HOBS) isolated from C.Discoideum (Cremanthodium discoidiem) on tumor cells were investigated.The results indicate that HOBS induced DNA damage and apoptosis of SMMCo7721 cells both in a dose-dependent manner as revealed by single cell gel electrophoresis and flow cytometry analyses,and the apoptosis rate was up to 38.9%.Measurement of ci(Ca2+) showed that HOBS could induce elevation of Ca2+.All the results suggest that HOBS could induce SMMC-7721 cells apoptosis.