CAJ | 학술논문

以舞毒蛾为材料,分离提取酚氧化酶,研究4-己基间苯二酚(4HR)和曲酸对舞毒蛾酚氧化酶活力的影响.结果表明:4HR对该虫的单酚酶和二酚酶活性均表现很强的抑制作用,其IC_(50)分别为0.000 41和0.000 35mmol·L~(-1).其中,4HR对单酚酶活力表达的迟滞时间有明显的延长效应,浓度为0.000 2 mmol·L~(-1)时可使单酚酶活力表达迟滞时间从181 s延长到253 s;而当浓度为0.000 5 mmol·L~(-1)时,其迟滞时间则延长至372 s.以邻苯二酚为底物时,4HR对二酚酶的抑制作用表现为典型的竞争型抑制类型,抑制常数K_1为0.000 15 mmol·L~(-1).曲酸对舞毒蛾的单酚酶和二酚酶活性均表现较强的抑制作用,其IC_(50)分别为0.06和0.92 mmol·L~(-1).曲酸对单酚酶活力表达的迟滞时间也有明显的延长效应,浓度为0.1 mmol·L~(-1)时可使单酚酶活力表达迟滞时间从306 s延长到732 s;而当浓度为0.15 mmol·L~(-1)时,其迟滞时间则延长至900 s.以邻苯二酚为底物时,曲酸对二酚酶的抑制作用表现为典型的竞争型抑制类型,抑制常数K_i为0.51 mmol·L~(-1).
이무독아위재료,분리제취분양화매,연구4-기기간분이분(4HR)화곡산대무독아분양화매활력적영향.결과표명:4HR대해충적단분매화이분매활성균표현흔강적억제작용,기IC_(50)분별위0.000 41화0.000 35mmol·L~(-1).기중,4HR대단분매활력표체적지체시간유명현적연장효응,농도위0.000 2 mmol·L~(-1)시가사단분매활력표체지체시간종181 s연장도253 s;이당농도위0.000 5 mmol·L~(-1)시,기지체시간칙연장지372 s.이린분이분위저물시,4HR대이분매적억제작용표현위전형적경쟁형억제류형,억제상수K_1위0.000 15 mmol·L~(-1).곡산대무독아적단분매화이분매활성균표현교강적억제작용,기IC_(50)분별위0.06화0.92 mmol·L~(-1).곡산대단분매활력표체적지체시간야유명현적연장효응,농도위0.1 mmol·L~(-1)시가사단분매활력표체지체시간종306 s연장도732 s;이당농도위0.15 mmol·L~(-1)시,기지체시간칙연장지900 s.이린분이분위저물시,곡산대이분매적억제작용표현위전형적경쟁형억제류형,억제상수K_i위0.51 mmol·L~(-1).
The inhibitory effects of tetra-hexylresorcinol and kojic acids on the phenoloxidase from Lymantria dispar were investigated. The results showed that the IC_(50) of tetra-hexylresorcinol was 0.000 41 mmol·L~(-1) for monophenolase activity and 0.000 35 mmol·L~(-1) for diphenolase, respectively. Tetra-hexylresorcinol extended the lagtime of the enzyme for oxidation of L-tyrosine. The lagtime extended from 181 s to 253 s by 4-hexylresorcinol with 0.000 2 mmol·L~(-1), and the lagtime extended from 181 s to 372 s by the chemical with 0.000 5 mmol·L~(-1). The inhibition kinetics analyzed by Lineweaver- Burk plots indicated that 4-hexylresorcinol was a competitive inhibitor for the oxidation of catechol and the inhibition constant was 0.000 15 mmol·L~(-1) .The IC_(50) of kojic acids were 0.06 mmol·L~(-1) for monophenolase activity and 0.92 mmol·L~(-1) for diphenolase,respectively. Kojic acids extended the lagtime of the enzyme for oxidation of L-tyrosine. The lagtime extended from 306 s to 702 s by the kojic acids with 0.1 mmol·L~(-1), and the lagtime extended from 306 s to 900 s by the compound with 0.15 mmol·L~(-1) .The inhibition kinetics analyzed by Lineweaver-Burk plots indicated that kojic acids was a competitive inhibitor for the oxidation of catechol, and the inhibition constant was 0.51 mmol·L~(-1).