CAJ | 학술논문

采用3对特异性引物,对中国近海常见3种原甲藻的18S rDNA的部分序列进行PCR扩增,运用变性梯度凝胶电泳技术(Denaturing Gradient Gel Electrophoresis, DGGE)对PCR产物进行了差异性分析.结果表明,各对引物具有不同的区分效果,选择包含较多差异碱基的短片段进行扩增,可以对藻种进行更好的区分;PCR-DGGE技术具有较高的灵敏性,不同藻种间即使1个碱基的差异也可以区分开;PCR-DGGE技术可以对3种原甲藻进行区分.
채용3대특이성인물,대중국근해상견3충원갑조적18S rDNA적부분서렬진행PCR확증,운용변성제도응효전영기술(Denaturing Gradient Gel Electrophoresis, DGGE)대PCR산물진행료차이성분석.결과표명,각대인물구유불동적구분효과,선택포함교다차이감기적단편단진행확증,가이대조충진행경호적구분;PCR-DGGE기술구유교고적령민성,불동조충간즉사1개감기적차이야가이구분개;PCR-DGGE기술가이대3충원갑조진행구분.
Three pairs of primers were applied for the amplification of 18S rDNA partial sequences of three species of Prorocentrum. PCR Products were analyzed by DGGE. Primers developed in this study showed different distinguishing effects. Short PCR amplicons with multiple species-specific differences in base pair composition could be separated by DGGE effectively. PCR-DGGE was a sensitive technique, even only a one-base difference between various species could be discerned. The results demonstrated that PCR-DGGE could be used in the difference analysis of these three species of Prorocentrum.