CAJ | 학술논문

用小花棘豆(Oxytropis glabra DC.)单株植物叶和茎切段做外植体,体外分离并培养内生真菌,研究菌落和分生孢子的微生物形态特征,对培养出的内生真菌的5.8SrDNA/ITS区进行扩增及序列测定,序列输入GenBank并与已发表的小花棘豆内生真菌序列进行同源性比对,并利用DNASTAR软件包中MegAlign软件构建系统发育树,结合分子生物学及微生物学研究推测内生真菌所属类群.结果显示:11株小花棘豆内生真菌的菌落和分生孢子的微生物形态特征与埃里砖格孢属真菌Embellisia sp.L12特征一致;小花棘豆内生真菌间ITS1和5.8SrDNA区序列相同,与Embellisia sp.L12相比只有一个变异位点,位于5.8SrDNA区,在ITS2区另有两个变异位点.推测小花棘豆内生真菌应与埃里砖格孢属亲缘关系密切,对其物种种属分类地位还须深入研究.
용소화극두(Oxytropis glabra DC.)단주식물협화경절단주외식체,체외분리병배양내생진균,연구균락화분생포자적미생물형태특정,대배양출적내생진균적5.8SrDNA/ITS구진행확증급서렬측정,서렬수입GenBank병여이발표적소화극두내생진균서렬진행동원성비대,병이용DNASTAR연건포중MegAlign연건구건계통발육수,결합분자생물학급미생물학연구추측내생진균소속류군.결과현시:11주소화극두내생진균적균락화분생포자적미생물형태특정여애리전격포속진균Embellisia sp.L12특정일치;소화극두내생진균간ITS1화5.8SrDNA구서렬상동,여Embellisia sp.L12상비지유일개변이위점,위우5.8SrDNA구,재ITS2구령유량개변이위점.추측소화극두내생진균응여애리전격포속친연관계밀절,대기물충충속분류지위환수심입연구.
Leaves and stems were cut from individual plants of Oxytropis glabra DC., and used as materials to isolate and culture the endophytic fungus in vitro.The microbiological morphology of colony and the conidiophore were investigated. The 5.8SrDNA/ITS of the in vitro cultured endophytic fungi were sequenced, and then the sequence data of the endophytic fungi were submitted to GenBank and analysed by homologous blast.The phylogenetic tree was constructed by MegAlign of DNASTAR software package.The molecular biological and microbiological characters were investigated in order to predict the classification of the endophytic fungi.The results indicated that the morphologic characteristics of the colony and conidiophore of this 11 endophytic fungi were the same as that of Embellisia sp.L12.The sequences of ITS1 and 5.8SrDNA of endophytic fungus from Oxytropis glabra DC. were totally the same. Compared to the sequence of Embellisia sp.L12,there was only one viriation site which was in the 5.8SrDNA region in each sequence.In addition, two variation sites were shown in the ITS2 region.It was presumed that the endophytic fungi had a close relationship to Embellisia, but the classification of the endophytic fungi need further research.