林业科学
林業科學
임업과학
SCIENTIA SILVAE SINICAE
2010年
4期
37-42
,共6页
钟浩%周明兵%白有煌%汤定钦
鐘浩%週明兵%白有煌%湯定欽
종호%주명병%백유황%탕정흠
毛竹%磁珠富集%转座子%TIRs%Stowaway-like%MITEs
毛竹%磁珠富集%轉座子%TIRs%Stowaway-like%MITEs
모죽%자주부집%전좌자%TIRs%Stowaway-like%MITEs
Phyllostachys edulis%enrichment by magnetic beads%transposon%TIRs%Stowaway-like MITEs
微型颠倒重复序列(miniature inverted repeat transposable elements,MITEs)是一类对基因组进化和基因表达有重要调节作用的转座子.为分析MITEs在毛竹基因组中的分布特性,借鉴Fast Isolation by AFLP of SequencesContaining repeats(FIASCO)方法,首次构建Stowaway-like MITEs富集文库.随机挑取21个克隆,测序发现2个含有Stowaway-like MITEs序列(Stow-Ph1和Stow-Ph2),阳性率为9.52%.Stow-Ph1和Stow-Ph2的长度分别为260和258 bp,具有Stowaway-like MITEs典型的末端颠倒重复序列(terminal inverted repeats,TIRs)和靶位点重复序列(target site duplication,TSD).Stow-Ph1和Stow-Ph2与水稻的Stow-Os8(FJ266024)的同源性分别为46.4%和52.6%.Stow-Ph1和Stow-Ph2与水稻16类Stowaway-like MITEs的TIRs前20 bp的相似性均高于50%,表明毛竹的Stowaway-like MITEs在TIRs上是高度保守的.
微型顛倒重複序列(miniature inverted repeat transposable elements,MITEs)是一類對基因組進化和基因錶達有重要調節作用的轉座子.為分析MITEs在毛竹基因組中的分佈特性,藉鑒Fast Isolation by AFLP of SequencesContaining repeats(FIASCO)方法,首次構建Stowaway-like MITEs富集文庫.隨機挑取21箇剋隆,測序髮現2箇含有Stowaway-like MITEs序列(Stow-Ph1和Stow-Ph2),暘性率為9.52%.Stow-Ph1和Stow-Ph2的長度分彆為260和258 bp,具有Stowaway-like MITEs典型的末耑顛倒重複序列(terminal inverted repeats,TIRs)和靶位點重複序列(target site duplication,TSD).Stow-Ph1和Stow-Ph2與水稻的Stow-Os8(FJ266024)的同源性分彆為46.4%和52.6%.Stow-Ph1和Stow-Ph2與水稻16類Stowaway-like MITEs的TIRs前20 bp的相似性均高于50%,錶明毛竹的Stowaway-like MITEs在TIRs上是高度保守的.
미형전도중복서렬(miniature inverted repeat transposable elements,MITEs)시일류대기인조진화화기인표체유중요조절작용적전좌자.위분석MITEs재모죽기인조중적분포특성,차감Fast Isolation by AFLP of SequencesContaining repeats(FIASCO)방법,수차구건Stowaway-like MITEs부집문고.수궤도취21개극륭,측서발현2개함유Stowaway-like MITEs서렬(Stow-Ph1화Stow-Ph2),양성솔위9.52%.Stow-Ph1화Stow-Ph2적장도분별위260화258 bp,구유Stowaway-like MITEs전형적말단전도중복서렬(terminal inverted repeats,TIRs)화파위점중복서렬(target site duplication,TSD).Stow-Ph1화Stow-Ph2여수도적Stow-Os8(FJ266024)적동원성분별위46.4%화52.6%.Stow-Ph1화Stow-Ph2여수도16류Stowaway-like MITEs적TIRs전20 bp적상사성균고우50%,표명모죽적Stowaway-like MITEs재TIRs상시고도보수적.
Transposable elements were a kind of DNA fragments movable in genomes.Miniature inverted repeat transposable elements (MITEs) were a class of short, widespread transposable elements with high copy number which played an important role in evolution of genome and regulation of gene expression.In order to analyze the distribution and characteristics of MITEs in Phyllostachys edulis genome, DNA library enriched for Stowaway-like MITEs was firstly constructed by the method of FIASCO (Fast Isolation by AFLP of Sequences Containing repeats).Totally 21 recombinant clones were randomly isolated with PCR method and then sequenced.Two clones (Stow-Ph1 and Stow-Ph2) were identified as Stowaway-like MITEs.The rate of positive clones was 9.52%.The fragment lengths of Stow-Ph1 and Stow-Ph2 were 260 bp and 258 bp, respectively.They had typical TIRs (terminal inverted repeats) and TSD (target site duplication) of Stowaway-like MITEs.The identity was 46.4% between Stow-Phi and Stow-Os8 (FJ266024), and 52.6% between Stow-Ph2 and Stow-Os8.The identities among Stow-Ph1, Stow-Ph2 and 16 rice Stowaway-like MITEs were above 50% based on the first 20 bp of their TIRs, which reflected that the TIRs of Stow-Ph1 and Stow-Ph2 were highly conserved.
