激光生物学报
激光生物學報
격광생물학보
ACTA LASER BIOLOGY SINICA
2012年
4期
360-364
,共5页
吴琪%周守标%程龙玲%吴国伟
吳琪%週守標%程龍玲%吳國偉
오기%주수표%정룡령%오국위
铜胁迫%芦竹%生长生理指标%富集能力
銅脅迫%蘆竹%生長生理指標%富集能力
동협박%호죽%생장생리지표%부집능력
Cu stress%Arundo donax%physiological index%accumulation
通过测定叶片中叶绿素含量、过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、脯氨酸含量及质膜透性,研究了不同浓度铜胁迫对芦竹幼苗植株的生理指标及富集能力的影响.结果表明:随铜处理浓度的增大,叶片中叶绿素含量、POD、SOD和CAT活性均呈先升高后降低的趋势,当铜处理浓度在100 mg·kg-1时,叶绿素含量、CAT活性达到最大值,而POD、SOD活性在200 mg·kg-1处理时达 到最大值.MDA含量、脯氨酸含量及质膜透性均随铜浓度增大而升高,当铜处理浓度在800 mg·kg-1时,叶片中的MDA含量、脯氨酸含量及质膜透性达到最大值.检测芦竹中铜含量的分布规律为根(地下部分)>茎、叶(地上部分),当铜处理浓度未达800 mg·kg-1时,转运系数小于1,富集系数大于1.结果表明芦竹对铜污染土壤具有一定耐受力.
通過測定葉片中葉綠素含量、過氧化氫酶(CAT)、過氧化物酶(POD)和超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、脯氨痠含量及質膜透性,研究瞭不同濃度銅脅迫對蘆竹幼苗植株的生理指標及富集能力的影響.結果錶明:隨銅處理濃度的增大,葉片中葉綠素含量、POD、SOD和CAT活性均呈先升高後降低的趨勢,噹銅處理濃度在100 mg·kg-1時,葉綠素含量、CAT活性達到最大值,而POD、SOD活性在200 mg·kg-1處理時達 到最大值.MDA含量、脯氨痠含量及質膜透性均隨銅濃度增大而升高,噹銅處理濃度在800 mg·kg-1時,葉片中的MDA含量、脯氨痠含量及質膜透性達到最大值.檢測蘆竹中銅含量的分佈規律為根(地下部分)>莖、葉(地上部分),噹銅處理濃度未達800 mg·kg-1時,轉運繫數小于1,富集繫數大于1.結果錶明蘆竹對銅汙染土壤具有一定耐受力.
통과측정협편중협록소함량、과양화경매(CAT)、과양화물매(POD)화초양화물기화매(SOD)활성、병이철(MDA)함량、포안산함량급질막투성,연구료불동농도동협박대호죽유묘식주적생리지표급부집능력적영향.결과표명:수동처리농도적증대,협편중협록소함량、POD、SOD화CAT활성균정선승고후강저적추세,당동처리농도재100 mg·kg-1시,협록소함량、CAT활성체도최대치,이POD、SOD활성재200 mg·kg-1처리시체 도최대치.MDA함량、포안산함량급질막투성균수동농도증대이승고,당동처리농도재800 mg·kg-1시,협편중적MDA함량、포안산함량급질막투성체도최대치.검측호죽중동함량적분포규률위근(지하부분)>경、협(지상부분),당동처리농도미체800 mg·kg-1시,전운계수소우1,부집계수대우1.결과표명호죽대동오염토양구유일정내수력.
To determine the tolerance and phytoaccumulation ability of Arundo donax to copper (Cu),the effects of exogenous Cu treatments on chlorophyll content,activities of catalase ( CAT),peroxidase (POD) and superoxide dismutase (SOD),malondialdehyde (MDA) content,protein content and plasma membrane permeability were examined.Chlorophyll content and the activities of CAT,POD and SOD increased firstly then declined significantly.When Cu concentration was 100 mg · kg1,the chlorophyll content and the activity of CAT were the highest,while the activities of POD and SOD reached maximum when Cu concentration was 200 mg · kg-1.MDA content,Pro content and plasma membrane permeability increased with increasing concentration,and maximality was found at 800 mg · kg-l Cu.The total Cu content was higher in roots (underground part) than in stems and leaves (aboveground part).The translocation factors of Cu were all below one,while the accumulation coefficient were above one,when plants were not subjected to less than 800 mg · kg-1Cu treatment.Our results suggested A.donax was a tolerant plant species to copper.