CAJ | 학술논문

目的通过观察血管紧张素转化酶抑制剂(ACEI)依那普利对肾性高血压大鼠脑缺血再灌注后脑组织基质金属蛋白酶(MMP)-2和MMP-9表达的影响,探讨依那普利的脑保护机制.方法 Wistar雄性大鼠28只,随机分为高血压组和正常血压组.前组采用狭窄肾动脉方法建立肾性高血压大鼠模型,再随机分为依那普利组(Y)和缺血再灌注组(HIR),分别采用依那普利2 mg/kg和0.9%氯化钠注射液进行灌胃治疗;正常血压组再分为假手术组(N)和缺血再灌注组(IR),分别行假手术和缺血再灌注处理.采用大脑中动脉线栓法造成大脑缺血再灌注模型,缺血时间为2 h,再灌注22 h.采用免疫组织化学技术检测脑组织MMP-2和MMP-9的表达,用图象分析仪测定灰度值.结果与N组比较,IR组MMP-2、MMP-9灰度值明显增高(P＜0.01);与IR组比较,HIR组MMP-2、MMP-9灰度值增高(P＜0.05);与HIR组比较,Y组MMP-2、MMP-9灰度值减少(均P＜0.01).结论高血压加重缺血再灌注后脑组织MMP-2和MMP-9的表达;依那普利能明显抑制脑组织MMP-2和MMP-9的表达.
목적 통과관찰혈관긴장소전화매억제제(ACEI)의나보리대신성고혈압대서뇌결혈재관주후뇌조직기질금속단백매(MMP)-2화MMP-9표체적영향,탐토의나보리적뇌보호궤제.방법 Wistar웅성대서28지,수궤분위고혈압조화정상혈압조.전조채용협착신동맥방법건립신성고혈압대서모형,재수궤분위의나보리조(Y)화결혈재관주조(HIR),분별채용의나보리2 mg/kg화0.9%록화납주사액진행관위치료;정상혈압조재분위가수술조(N)화결혈재관주조(IR),분별행가수술화결혈재관주처리.채용대뇌중동맥선전법조성대뇌결혈재관주모형,결혈시간위2 h,재관주22 h.채용면역조직화학기술검측뇌조직MMP-2화MMP-9적표체,용도상분석의측정회도치.결과 여N조비교,IR조MMP-2、MMP-9회도치명현증고(P＜0.01);여IR조비교,HIR조MMP-2、MMP-9회도치증고(P＜0.05);여HIR조비교,Y조MMP-2、MMP-9회도치감소(균P＜0.01).결론 고혈압가중결혈재관주후뇌조직MMP-2화MMP-9적표체;의나보리능명현억제뇌조직MMP-2화MMP-9적표체.
Objective To observe the effects of ACEI( enalapril) on expression of MMP-2 and MMP-9 in renal hypertension rats with focal cerebral ischemia-reperfusion injury. Methods Twenty-eight Wistar male rats were randomly divided into two groups: hypertension group and normaltension group. The former which formed model of renal hypertension rats by constricted renovascular were randomly divided into enalapril group (Y) and hypertension ischemia and reperfusion group(HIR),which fed with enalapril 2mg/kg and equal volum saline respectively;The latter were divided into sham-operation group ( N) and normaltension ischemia and reperfusion group (IR). The focal cerebral ischemia model was established in Wistar rats by using the method of thread inserting left middle cerebral artery occlusion(MCAO) for 2h. After ischemic 24h,the expression of MMP-2 and MMP-9 were measured by immunohisto-chemistry,and the gray scale value was measured by imaging analysis. Results Compared with N,the gray scale values of MMP-2 and MMP-9 in IR were higher(P <0. 01); Compared with IR, the gray scale values of MMP-2 and MMP-9 in HIR were higher(P<0. 05);Compared with HIR,the gray scale values of MMP-2 and MMP-9 in Y were lower(P<0.01). Conclusion Hypertension can increase the expression of MMP-2 and MMP-9 in rats with focal cerebral ischemia-reperfusion. ACEI (Enalapril) could inhibit the expression of MMP-2 and MMP-9 in renal hypertension rats with focal cerebral ischemia-reperfusion.