CAJ | 학술논문

目的探讨苏州地区儿童急性呼吸道人类偏肺病毒感染的流行特点及与气候因素的关系.方法对2006-2009年因呼吸道感染而住院的6655例儿童应用逆转录PCR(RT-PCR)方法检测人类偏肺病毒(hMPV),同时采用直接免疫荧光法,检测呼吸道分泌物中呼吸道合胞病毒、流感病毒(A、B)、副流感病毒(Ⅰ～Ⅲ)和腺病毒;同时收集2006-2009年苏州地区月平均气温、月平均湿度、月总雨量、月总日照、月平均风速等有关气象资料,采用直线回归和逐步回归法研究各病毒活性与气候因素的相关性.结果 2006-2009年苏州地区病毒总检出率为32.2%(2142/6599),其中hMPV检出率为8.9%(596/6655),RSV检出率(15.7%)高于hMPV检出率,流感病毒、副流感病毒和腺病毒检出率均低于hMPV检出率.2006-2009年hMPV年度检出率分别为8.2%(135/1646),8.1%(137/1682),12.7%(184/1444),7.4%(140/1883),差异有统计学意义(x2=33.23,P＜0.05).hMPV四季检出率分别为11.6%、7.6%、4.7%和11.7%,冬春季的检出率高于夏秋季(x2=74.67,P＜0.001).hMPV检出率与月平均气温之间为中度相关(r=-0.43),与月平均雨量(r=-0.29)、月平均相对湿度(r=-0.27)、月平均日照时间(r=-0.11)、月平均风速(r=-0.13)为低度相关.结论 hMPV是引起苏州地区儿童呼吸道感染第二位的病毒病原,流行季节为冬春季,气候因素尤其是气温和雨量对hMPV的流行可能有影响.
목적 탐토소주지구인동급성호흡도인류편폐병독감염적류행특점급여기후인소적관계.방법 대2006-2009년인호흡도감염이주원적6655례인동응용역전록PCR(RT-PCR)방법검측인류편폐병독(hMPV),동시채용직접면역형광법,검측호흡도분비물중호흡도합포병독、류감병독(A、B)、부류감병독(Ⅰ～Ⅲ)화선병독;동시수집2006-2009년소주지구월평균기온、월평균습도、월총우량、월총일조、월평균풍속등유관기상자료,채용직선회귀화축보회귀법연구각병독활성여기후인소적상관성.결과 2006-2009년소주지구병독총검출솔위32.2%(2142/6599),기중hMPV검출솔위8.9%(596/6655),RSV검출솔(15.7%)고우hMPV검출솔,류감병독、부류감병독화선병독검출솔균저우hMPV검출솔.2006-2009년hMPV년도검출솔분별위8.2%(135/1646),8.1%(137/1682),12.7%(184/1444),7.4%(140/1883),차이유통계학의의(x2=33.23,P＜0.05).hMPV사계검출솔분별위11.6%、7.6%、4.7%화11.7%,동춘계적검출솔고우하추계(x2=74.67,P＜0.001).hMPV검출솔여월평균기온지간위중도상관(r=-0.43),여월평균우량(r=-0.29)、월평균상대습도(r=-0.27)、월평균일조시간(r=-0.11)、월평균풍속(r=-0.13)위저도상관.결론 hMPV시인기소주지구인동호흡도감염제이위적병독병원,류행계절위동춘계,기후인소우기시기온화우량대hMPV적류행가능유영향.
Objective To study the epidemiology of human metapneumovirus (hMPV) infection in children and its relations with meteorological conditions in Suzhou. Method Samples obtained from 6655 children hospitalized with acute respiratory tract infections (ARIs) during the period from 2006 to 2009,were tested for virus pathogens. Nasopharyngeal aspirates were obtained from the children according to a standard protocol and were tested for respiratory syncytial virus ( RSV), influenza viruses (IFV) A and B,parainfluenza virus (PIV) types 1,2, and 3 and adenovirus (ADV) with direct immunofluorescence assay.Samples were tested for hMPV with reverse transcription polymerase chain reaction (RT-PCR).Meteorological conditions including mean temperature, relative humidity, rainfall amount, sum of sunshine and mean wind velocity were collected monthly. The relationship between activity of the virus and meteorological conditions was analyzed by linear regression and stepwise regression analysis. Result Viral pathogens were identified in 32. 2% of 6655 specimens. The positive rate of hMPV was 8.9%, RSV was 15.7%, IFV, PIV and ADV detection rates were less than that of hMPV. The annual positive rate of hMPV from 2006 to 2009 was 8.2%, 8. 1%, 12.7%, 7.4% respectively(x2 =33.23, P＜0.05). The hMPV positive rate of the four seasons was 11.6% , 7.6%, 4. 7% and 11.7%, respectively, detection rate in winter and spring was significantly higher than those in summer and autumn( x2 = 74. 67, P ＜ 0. 001 ). The positive rate of hMPV and the monthly mean temperature was moderately correlated ( r = - 0. 43 ), and the monthly average rainfall ( r = - 0. 29 ), monthly mean relative humidity ( r = - 0. 27 ), monthly average sunshine duration ( r = - 0. 11 ), the monthly average wind speed ( r = - 0. 13 ) had low correlations.Conclusion hMPV was the sceond most common viral pathogen of acute respiratory tract infection in children in Suzhou, which prevailed predominantly in the winter and spring. Climatic factors, especially temperature and rainfall may affect the prevalence of hMPV.