中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2008年
7期
480-484
,共5页
田孝东%杨尹默%汤坚强%万远廉%黄莛庭
田孝東%楊尹默%湯堅彊%萬遠廉%黃莛庭
전효동%양윤묵%탕견강%만원렴%황정정
胰腺肿瘤%Hedgehog%免疫组化%Western blot
胰腺腫瘤%Hedgehog%免疫組化%Western blot
이선종류%Hedgehog%면역조화%Western blot
Pancreatic neoplasm%Hedgehog%Immunohistochemistry%Western blot
目的 研究Hedgehog相关信号蛋白Ihh、Ptc和Smo在胰腺癌中的表达情况及其临床意义.方法 采用免疫组织化学方法 检测54例原发性胰腺癌及5例正常胰腺石蜡标本中Hedgehog信号蛋白(Ihh、Ptc、Smo)的表达情况,并统计分析其阳性率与临床病理特征的相关性;采用Westernblot比较21例新鲜胰腺癌及癌旁胰腺组织标本Hedgehog信号蛋白表达量的差异.结果 54例胰腺癌标本中,Ihh、Ptc及Smo阳性率分别为70.4%、64.8%和88.9%,5例正常胰腺组织中均无阳性表达;Ptc阳性率与肿瘤大小、分化程度、淋巴结转移情况及肿瘤TNM分期显著相关,Smo表达率则与肿瘤分化程度显著相关(P<0.05);21例新鲜胰腺癌标本中Ihh、Ptc、Smo平均表达量均显著高于癌旁胰腺组织(P<0.05).结论 胰腺癌组织中Hedgehog信号蛋白表达量显著增加;Hedgehog信号蛋白与胰腺癌临床病理特征相关.
目的 研究Hedgehog相關信號蛋白Ihh、Ptc和Smo在胰腺癌中的錶達情況及其臨床意義.方法 採用免疫組織化學方法 檢測54例原髮性胰腺癌及5例正常胰腺石蠟標本中Hedgehog信號蛋白(Ihh、Ptc、Smo)的錶達情況,併統計分析其暘性率與臨床病理特徵的相關性;採用Westernblot比較21例新鮮胰腺癌及癌徬胰腺組織標本Hedgehog信號蛋白錶達量的差異.結果 54例胰腺癌標本中,Ihh、Ptc及Smo暘性率分彆為70.4%、64.8%和88.9%,5例正常胰腺組織中均無暘性錶達;Ptc暘性率與腫瘤大小、分化程度、淋巴結轉移情況及腫瘤TNM分期顯著相關,Smo錶達率則與腫瘤分化程度顯著相關(P<0.05);21例新鮮胰腺癌標本中Ihh、Ptc、Smo平均錶達量均顯著高于癌徬胰腺組織(P<0.05).結論 胰腺癌組織中Hedgehog信號蛋白錶達量顯著增加;Hedgehog信號蛋白與胰腺癌臨床病理特徵相關.
목적 연구Hedgehog상관신호단백Ihh、Ptc화Smo재이선암중적표체정황급기림상의의.방법 채용면역조직화학방법 검측54례원발성이선암급5례정상이선석사표본중Hedgehog신호단백(Ihh、Ptc、Smo)적표체정황,병통계분석기양성솔여림상병리특정적상관성;채용Westernblot비교21례신선이선암급암방이선조직표본Hedgehog신호단백표체량적차이.결과 54례이선암표본중,Ihh、Ptc급Smo양성솔분별위70.4%、64.8%화88.9%,5례정상이선조직중균무양성표체;Ptc양성솔여종류대소、분화정도、림파결전이정황급종류TNM분기현저상관,Smo표체솔칙여종류분화정도현저상관(P<0.05);21례신선이선암표본중Ihh、Ptc、Smo평균표체량균현저고우암방이선조직(P<0.05).결론 이선암조직중Hedgehog신호단백표체량현저증가;Hedgehog신호단백여이선암림상병리특정상관.
Objective To investigate the expression of hedgehog signal molecules (Ihh,Ptc and Stop) in human pancreatic cancer and their correlation with clinical and pathologic characteristics.Methods Fifty-four pancreatic cancer samples and 5 normal pancreatic tissues were examined by im-munohistochemistry for the expression and localization of hedgehog signal molecules (Ihh, Ptc andStop). Meanwhile,correlation of hedgehog signal molecules to clinical and pathologic characteristics ofpancreatic cancer was analyzed. Western blot was carried out to analyze the expression of Ihh,Ptc and Smo in 21 freshly removed pancreatic cancer tissues and paraneoplastic pancreatic tissues. Results Of the 54 pancreatic cancer samples, immunoreactivity of Ihh, Ptc and Smo was observed in 70. 4%,64.8% and 88.9 % of the cancer tissues, respectively. No immunoreaetivity was observed in the ductal cells of all the 5 normal pancreas tissues. Expression of Ptc was strongly correlated with tumor size,differentiation grade of tumor cells,lymph node metastasis and TNM stage of tumor,and Stop expres-sion strongly correlated with the differentiated grade of tumor cells (P~0. 05,respectively). Western blot analysis revealed that the expression of Ihh, Ptc and Stop was significantly higher in pancreatic cancer compared to paraneoplastic pancreatic tissues (P < 0. 05). Conclusion The expression of Hedgehog signal molecules is significantly higher in pancreatic cancer tissues. These molecules are correlated to clinical and pathologic characteristics of pancreatic cancer.
