CAJ | 학술논문

目的了解Semaphorins-3A蛋白在生后发育期大鼠脑中的分布特征和表达变化.方法随机选择生后0d、7d、14d、21d和28d日龄SD大鼠,通过免疫组化法观察Semaphorins-3A阳性细胞在大脑皮层、海马、齿状回和内嗅皮层内的分布和表达量.结果出生后大鼠Semaphorins-3A阳性细胞广泛密集的分布于顶、枕、额、颞、岛叶、扣带皮质、梨状前皮质和杏仁周皮质中除分子层(Ⅰ)以外的颗粒细胞层(Ⅱ)、外椎体细胞层(Ⅲ)、内颗粒细胞层(Ⅳ)、内椎体细胞层(Ⅴ)和多行层(Ⅵ).Semaphorins-3A蛋白在0d、7d、14d、21d和28d日龄大鼠的内嗅皮层中表达量分别为84916.23±3266.34、77711.41±2634.26、74124.25±3989.09、65887.63±3406.57和57705.96±3136.35(IOD),表达区域缩小在Ⅱ～Ⅵ层的部分层次.Semaphorins-3A阳性细胞也分布于各日龄大鼠的齿状回、CA1、CA3区颗粒细胞层,少数散在分布于多行层,齿状回、CA1、CA3区内Semaphorins-3A表达量在各日龄之间具有明显差异(P＜0.01).结论 Semaphorins-3A阳性细胞广泛分布于发育期大鼠脑各脑叶及海马CA1、CA3、DG区内,并随年龄的增长分布范围逐渐缩小.
목적 료해Semaphorins-3A단백재생후발육기대서뇌중적분포특정화표체변화.방법 수궤선택생후0d、7d、14d、21d화28d일령SD대서,통과면역조화법관찰Semaphorins-3A양성세포재대뇌피층、해마、치상회화내후피층내적분포화표체량.결과 출생후대서Semaphorins-3A양성세포엄범밀집적분포우정、침、액、섭、도협、구대피질、리상전피질화행인주피질중제분자층(Ⅰ)이외적과립세포층(Ⅱ)、외추체세포층(Ⅲ)、내과립세포층(Ⅳ)、내추체세포층(Ⅴ)화다행층(Ⅵ).Semaphorins-3A단백재0d、7d、14d、21d화28d일령대서적내후피층중표체량분별위84916.23±3266.34、77711.41±2634.26、74124.25±3989.09、65887.63±3406.57화57705.96±3136.35(IOD),표체구역축소재Ⅱ～Ⅵ층적부분층차.Semaphorins-3A양성세포야분포우각일령대서적치상회、CA1、CA3구과립세포층,소수산재분포우다행층,치상회、CA1、CA3구내Semaphorins-3A표체량재각일령지간구유명현차이(P＜0.01).결론 Semaphorins-3A양성세포엄범분포우발육기대서뇌각뇌협급해마CA1、CA3、DG구내,병수년령적증장분포범위축점축소.
Objective To study the distribution and expression of Semaphorins-3A protein in brain of postnatal rats.Methods Semaphorins-3A positive cells were observed by immunohistochemistry in the cerebral cortex,hippocampus,dentate gyms and entorhinal cortex in postnatal 0d,7d,14d,21 d and 28d of Sprague Dawley rats.Results Semaphorins-3A positive cells widely distributed in the granule cell layer ( Ⅱ ),external pyramidal cell layer ( Ⅲ ),internal granular cell layer ( Ⅳ ),pyramidal cell layer ( Ⅴ ) and layer of polymorphous cells ( Ⅵ ),in addition to the molecular layer ( Ⅰ ) of the parietal,occipital,frontal,temporal,insular,cingulate cortex,piriform cortex and entorhinal cortex with postnatal 0d,7d,14d,21d and 28d rats.The amount of semaphorins-3A positive cells(IOD) in the entorhinal cortex was 84916.23 ± 3266.34 in P0d,77711.41 ± 2634.26 in P7d,74124.25 ± 3989.09 in P14d,65887.63 ± 3406.57 in P21d and 57705.96 ± 3136.35 in P28d,meanwhile the region of semaphorins-3A positive cells narrowed in the part level with Ⅱ -Ⅵ levels of cortex.Similarly semaphorins-3A positive cells distributed mainly in granule cell layer of dentate gyrus,CA1,CA3 region and only a few of semaphorins-3A positive cells scattered in the multi-line layer in hippocampus.The expression level of semaphorins-3Awas significant difference among postnatal 0d,7d,14d,21d and 28d rats (P＜0.01).Conclusion Semaphorins-3A positive cells widely distribute in the various cortex and hippocampus in developing rat brain,and the region of semaphorins-3A is reduced with age growth of rats.