中华内分泌代谢杂志
中華內分泌代謝雜誌
중화내분비대사잡지
CHINESE JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2011年
6期
505-508
,共4页
伍丽萍%施秉银%杨婧%旬利茹%徐利%田竹芳%高珊%张煜
伍麗萍%施秉銀%楊婧%旬利茹%徐利%田竹芳%高珊%張煜
오려평%시병은%양청%순리여%서리%전죽방%고산%장욱
性别差异%格雷夫斯病%诱导%小鼠%模型
性彆差異%格雷伕斯病%誘導%小鼠%模型
성별차이%격뢰부사병%유도%소서%모형
Gender difference%Graves′ disease%Induce%Mice%Model
目的 在雌性或雄性BALB/c小鼠制备Graves病动物模型,观察其促甲状腺素受体抗体(TRAb)滴度,TT4水平以及甲状腺组织的增生情况,探讨性别差异对成模的影响.方法 用表达TSH受体A亚单位的重组腺病毒免疫雌性或雄性BALB/c小鼠诱导Graves病,每3周免疫1次,共3次,末次免疫后4周取血,检测TRAb、TT4,剥离甲状腺行组织学检查.结果 雌性和雄性实验组小鼠TRAb阳性率均达到100%,两组之间抗体滴度无显著性差异.雌性及雄性实验组分别有75.0%,41.7%的小鼠发生甲亢,雌性实验组TT4水平明显高于雄性实验组并有统计学差异(P＜0.01),各组甲亢小鼠甲状腺组织出现明显增生.结论 利用表达TSH受体A亚单位的重组腺病毒免疫雌性、雄性BALB/c小鼠诱导Graves病动物模型,尽管两性TRAb抗体水平相似,但其甲亢发生率及程度存在一定的性别偏倚,提示雌性小鼠更易于模型的诱导.
目的 在雌性或雄性BALB/c小鼠製備Graves病動物模型,觀察其促甲狀腺素受體抗體(TRAb)滴度,TT4水平以及甲狀腺組織的增生情況,探討性彆差異對成模的影響.方法 用錶達TSH受體A亞單位的重組腺病毒免疫雌性或雄性BALB/c小鼠誘導Graves病,每3週免疫1次,共3次,末次免疫後4週取血,檢測TRAb、TT4,剝離甲狀腺行組織學檢查.結果 雌性和雄性實驗組小鼠TRAb暘性率均達到100%,兩組之間抗體滴度無顯著性差異.雌性及雄性實驗組分彆有75.0%,41.7%的小鼠髮生甲亢,雌性實驗組TT4水平明顯高于雄性實驗組併有統計學差異(P＜0.01),各組甲亢小鼠甲狀腺組織齣現明顯增生.結論 利用錶達TSH受體A亞單位的重組腺病毒免疫雌性、雄性BALB/c小鼠誘導Graves病動物模型,儘管兩性TRAb抗體水平相似,但其甲亢髮生率及程度存在一定的性彆偏倚,提示雌性小鼠更易于模型的誘導.
목적 재자성혹웅성BALB/c소서제비Graves병동물모형,관찰기촉갑상선소수체항체(TRAb)적도,TT4수평이급갑상선조직적증생정황,탐토성별차이대성모적영향.방법 용표체TSH수체A아단위적중조선병독면역자성혹웅성BALB/c소서유도Graves병,매3주면역1차,공3차,말차면역후4주취혈,검측TRAb、TT4,박리갑상선행조직학검사.결과 자성화웅성실험조소서TRAb양성솔균체도100%,량조지간항체적도무현저성차이.자성급웅성실험조분별유75.0%,41.7%적소서발생갑항,자성실험조TT4수평명현고우웅성실험조병유통계학차이(P＜0.01),각조갑항소서갑상선조직출현명현증생.결론 이용표체TSH수체A아단위적중조선병독면역자성、웅성BALB/c소서유도Graves병동물모형,진관량성TRAb항체수평상사,단기갑항발생솔급정도존재일정적성별편의,제시자성소서경역우모형적유도.
Objective To investigate the effect of mice gender on the TSH receptor antibody(TRAb)titers, the levels of TT4,and the degree of thyroid hyperplasia by establishing an animal model of Graves′ disease in male and female BALB/c mice. Methods Male and female BALB/c mice were immunized with recombinant adenovirus expressing TSHRA subunit(Ad-TSHR289)to induce Graves′ disease. Animals were injected 3 times at intervals of 3 weeks. All mice were sacrificed 4 weeks after the last injection to obtain blood for measurement of TSHR antibody titers and TT4evels, and thyroid glands for histological examination. Results TRAb positive rates were 100% both in female or male mice. No significant difference was observed in titers of TRAb between them. The incidence of hyperthyroidism in female mice was higher than that in male mice, being 75.0% and 41.7% respectively. There was statistical difference in levels of TT4between females and males(P＜0.01). Mice with high TT4exihibited marked thyroid hyperplasia. Conclusion Despite TSHR antibodies were similar between female and male mice, the incidence and degree of hyperthyroidism showed sex bias in Graves′s animal model. The results indicated that it was easier to induce model in females than in males by immunizing BALB/c mice with Ad-TSHR289.
