中华神经外科杂志
中華神經外科雜誌
중화신경외과잡지
Chinese Journal of Neurosurgery
2010年
2期
116-119
,共4页
印红霞%周梦良%杭春华%祁晓平%史继新
印紅霞%週夢良%杭春華%祁曉平%史繼新
인홍하%주몽량%항춘화%기효평%사계신
脑血管痉挛%蛛网膜下腔出血%氧合血红蛋白%平滑肌细胞%核因子-κB
腦血管痙攣%蛛網膜下腔齣血%氧閤血紅蛋白%平滑肌細胞%覈因子-κB
뇌혈관경련%주망막하강출혈%양합혈홍단백%평활기세포%핵인자-κB
Cerebral vasopasm%Subarachnoid hemorrhage%Oxyhemoglobin%Vascular smooth muscle cells%NF -κB
目的 观察氧合血红蛋白(OxyHb)对培养的血管平滑肌细胞(VSMCs)细胞密度及核因子(NF)-κB的DNA结合活性的影响.方法 用不同浓度(1～100 μmol/L)的OxyHb处理VSMCs,观察其形态学及细胞密度变化,测定NF-κB的DNA结合活性.结果 OxyHb刺激后VSMCs都表现出形态学的改变,其严重程度有浓度依赖性.但是在24 h时只有100 μmol/L组有明显的细胞数量减少,10μmol/L组在48 h时细胞数量开始明显减少.NF-κB的DNA结合活性在经OxyHb处理后明显增高,以10μmol/L及50μmol/L组最高.结论 NF-κB的DNA结合活性在经OxyHb处理的VSMCs中有明显改变,揭示了NF-κB在脑血管痉挛发生时是脑血管平滑肌细胞内重要的信号通路.
目的 觀察氧閤血紅蛋白(OxyHb)對培養的血管平滑肌細胞(VSMCs)細胞密度及覈因子(NF)-κB的DNA結閤活性的影響.方法 用不同濃度(1～100 μmol/L)的OxyHb處理VSMCs,觀察其形態學及細胞密度變化,測定NF-κB的DNA結閤活性.結果 OxyHb刺激後VSMCs都錶現齣形態學的改變,其嚴重程度有濃度依賴性.但是在24 h時隻有100 μmol/L組有明顯的細胞數量減少,10μmol/L組在48 h時細胞數量開始明顯減少.NF-κB的DNA結閤活性在經OxyHb處理後明顯增高,以10μmol/L及50μmol/L組最高.結論 NF-κB的DNA結閤活性在經OxyHb處理的VSMCs中有明顯改變,揭示瞭NF-κB在腦血管痙攣髮生時是腦血管平滑肌細胞內重要的信號通路.
목적 관찰양합혈홍단백(OxyHb)대배양적혈관평활기세포(VSMCs)세포밀도급핵인자(NF)-κB적DNA결합활성적영향.방법 용불동농도(1～100 μmol/L)적OxyHb처리VSMCs,관찰기형태학급세포밀도변화,측정NF-κB적DNA결합활성.결과 OxyHb자격후VSMCs도표현출형태학적개변,기엄중정도유농도의뢰성.단시재24 h시지유100 μmol/L조유명현적세포수량감소,10μmol/L조재48 h시세포수량개시명현감소.NF-κB적DNA결합활성재경OxyHb처리후명현증고,이10μmol/L급50μmol/L조최고.결론 NF-κB적DNA결합활성재경OxyHb처리적VSMCs중유명현개변,게시료NF-κB재뇌혈관경련발생시시뇌혈관평활기세포내중요적신호통로.
Objective To investigate the effect of oxyhemoglobin (OxyHb) on the cell density and DNA - binding activity of nuclear factor (NF) - κB in the cultured vascular smooth muscle cells(VSMCs). Methods The cultured VSMCs were treated with different concentration from 1 to 100 μmol/L of OxyHb. The cell density was measured. DNA - binding activity of NF -κB was detected using electrophoretic mobility shift assay (EMSA). Results Morphological alterations occurred in the VSMCs after treatment with OxyHb. The concentration of OxyHb at 100 μmol/L produced a significant decrease in cell density at 24 hour, and 10 μmol/L at 72 hour. DNA - binding activity of NF -κB was increased after treatment with OxyHb, and peaked at 10 and 50 μmol/L Conclusion Alteration of NF -κB DNA - binding activity occurred in the VSMCs treating with OxyHb, which suggests that NF -κB pathway in the cerebrovascular smooth muscle cells may be involved in the pathogenesis of cerebral vasospasm.
