CAJ | 학술논문

目的探讨谷氨酰胺(glutamine,GLN)对急性肝损伤大鼠肠黏膜屏障的作用.方法 61只清洁级Wistar大鼠随机分为3组:对照组、模型组、GLN干预组.采用脂多糖(lipopolysaccharide,LPS)和D-半乳糖胺(D-galactosamine,D-Gal)腹腔注射法建立急性肝损伤大鼠模型.检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)和总胆红素(TBiL)水平;光镜下及电镜下观察大鼠肝脏及肠道病理学改变;末端脱氧核苷酸转移酶(TdT)介导的缺口末端标记技术(TUNEL)检测细胞凋亡;高压液相离子色谱仪(HPLC-PED)检测大鼠肠黏膜通透性的改变.结果模型组及GLN干预组大鼠死亡率差异无统计学意义(P>0.05);模型组及GLN干预组大鼠血清ALT、AST和TBIL水平差异无统计学意义(P>0.05);三组肠黏膜通透性差异无统计学意义(P>0.05);光镜下GLN干预组与模型组大鼠肝脏损伤程度差异无统计学意义;肠道未见明显病变;透射电镜下模型组和GLN干预组大鼠肝脏和肠道出现线粒体和细胞核等损伤,GLN干预组较模型组大鼠损伤轻;GLN干预组肝脏和肠道的凋亡指数(Apoptosis index,AI)显著低于模型组(P<0.05).结论脂多糖可以造成D-半乳糖胺致敏大鼠急性肝损伤模型,造模前给予谷胺酰胺干预可能对于改善肝功能,保护肠黏膜的通透性,降低死亡率没有显著性作用.
목적 탐토곡안선알(glutamine,GLN)대급성간손상대서장점막병장적작용.방법 61지청길급Wistar대서수궤분위3조:대조조、모형조、GLN간예조.채용지다당(lipopolysaccharide,LPS)화D-반유당알(D-galactosamine,D-Gal)복강주사법건립급성간손상대서모형.검측혈청병안산전안매(ALT)、천동안산전안매(AST)화총담홍소(TBiL)수평;광경하급전경하관찰대서간장급장도병이학개변;말단탈양핵감산전이매(TdT)개도적결구말단표기기술(TUNEL)검측세포조망;고압액상리자색보의(HPLC-PED)검측대서장점막통투성적개변.결과 모형조급GLN간예조대서사망솔차이무통계학의의(P>0.05);모형조급GLN간예조대서혈청ALT、AST화TBIL수평차이무통계학의의(P>0.05);삼조장점막통투성차이무통계학의의(P>0.05);광경하GLN간예조여모형조대서간장손상정도차이무통계학의의;장도미견명현병변;투사전경하모형조화GLN간예조대서간장화장도출현선립체화세포핵등손상,GLN간예조교모형조대서손상경;GLN간예조간장화장도적조망지수(Apoptosis index,AI)현저저우모형조(P<0.05).결론 지다당가이조성D-반유당알치민대서급성간손상모형,조모전급여곡알선알간예가능대우개선간공능,보호장점막적통투성,강저사망솔몰유현저성작용.
Objective To explore the role of glutamine in LPS and D-Gal induced acute hepatic injury. Methods A total of 61 Wistar rats were randomly divided into three groups:control group, model group and GLN pretreated group.The animal model was established by LPS and D-Gal intraperitoneal injection. GLN at dose of 1 g/kg was intragastrically administrated for 7 d before intraperitoneal injection. To evaluate the hepatic injury, the serum alanine aminotransferase(ALT), aspartate aminotransferase(AST) and total bilimbin(TBiL) were detected by automatic biochemistry analysator. The liver and bowel tissue was observed by lightmicroscope and transmission electron microscope(TEM). The apoptosis of hepatocyte was detected by TUNEL. HPLC-PED was used in the study of intestinal permeability. Result No significant differences were noted between ALT, AST, TBIL level, death rate and intestinal permeability(L/M) between model group and GLN pretreated group;In microscope, the confused structure of hepatic injury and inflammatory infiltration were similar between model group and GLN pretreated greup.The injury of bowel was not obviously. Compared with the model group, there was better trend in liver and bowel in GLN pretreated group by transmission electron microscope(TEM) .The apoptosis index in GLN pretreated group were lower than those in model group. Conclusion LPS can induce acute liver injury in D-Gal-sensitized rats. Glutamine has't the trend of protecting liver function and intestinal barrier function, decreasing death rates.