CAJ | 학술논문

目的检测基质细胞衍生因子-1(SDF-1)、CDM在乒乓球拍样皮瓣组织不同时间、不同面积的表达,观察SDF-1在皮瓣组织血管新生中的作用.方法制作乒乓球拍样皮瓣动物模型.对皮瓣进行大体观察、皮瓣成活情况、免疫组织化学及酶联免疫吸附试验(ELISA)技术检测皮瓣远端组织在术后0、3、5、7、14 d时SDF-1、CD34的表达.结果 (1)同一组狭长窄蒂皮瓣,随着时间推移,SDF-1、CD34的表达也随之增加,SDF-1在第5天达到最高值(A组124.80±4.05,B组137.85±3.03,C组166.53±2.98,D组72.80±2.63,E组62.79±2.20),CD34在第7天达到最高值(A组16.76±0.62,B组17.60±0.72,C组18.48±0.55,D组12.70±0.60,E组11.51±0.70),随后逐渐下降.(2)不同组狭长窄蒂皮瓣,随着皮瓣面积的增大,SDF-1、CD34的表达也随之增加,当皮瓣面积达5 cm×5 cm时,各项指标不再增加,皮瓣远端部分坏死.结论在皮瓣成活过程中SDF-1和CD34的表达呈正相关.
목적 검측기질세포연생인자-1(SDF-1)、CDM재핑퐁구박양피판조직불동시간、불동면적적표체,관찰SDF-1재피판조직혈관신생중적작용.방법 제작핑퐁구박양피판동물모형.대피판진행대체관찰、피판성활정황、면역조직화학급매련면역흡부시험(ELISA)기술검측피판원단조직재술후0、3、5、7、14 d시SDF-1、CD34적표체.결과 (1)동일조협장착체피판,수착시간추이,SDF-1、CD34적표체야수지증가,SDF-1재제5천체도최고치(A조124.80±4.05,B조137.85±3.03,C조166.53±2.98,D조72.80±2.63,E조62.79±2.20),CD34재제7천체도최고치(A조16.76±0.62,B조17.60±0.72,C조18.48±0.55,D조12.70±0.60,E조11.51±0.70),수후축점하강.(2)불동조협장착체피판,수착피판면적적증대,SDF-1、CD34적표체야수지증가,당피판면적체5 cm×5 cm시,각항지표불재증가,피판원단부분배사.결론 재피판성활과정중SDF-1화CD34적표체정정상관.
Objective To detect the expression of stromal cell-derived factor 1 (SDF-1), CD34 in the PingPong Racket flap during the survival process, and investigate the role of SDF-1 in flap neovascularization. Methods The PingPong Racket flap animal model was established. The morphology and survival of the flap were observed. The expression of SDF-1 and CD34 in the distal flap was detected by using immunohistochemistry and enzyme-linked immunosorbent assay at 0, 3, 5, 7, and 14 days after operation respectively. Results (1) In the same group of flaps, with prolongation of time, the expression of SDF-1 and CD34 was increased. The expression of SDF-1 reached the peak at the 5th day (group A; 124. 80 ±4.05, B: 137.85±3.03, C: 166.53±2.98, D: 72. 80 ±2.63, E: 62.79±2.20), and that of CD34 reached the peak at the 7th day (group A: 16. 76 ±0.62, B: 17. 60 ±0.72, C: 18.48 ±0.55, D: 12.70 ±0. 60, E: 11. 51 ±0. 70) ; (2) In the different groups of flaps, when the flap area was increased, the expression of SDF-1 and CD34 was increased, and at the area of 5 cm ×5 cm, the flaps had partial necrosis. Conclusion There was a positive relationship between SDF-1 and CD34 in the survival process of the flap.