中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2012年
8期
570-572
,共3页
孟庆楠%王益民%王蕴华%刘彦强%孙秀岩
孟慶楠%王益民%王蘊華%劉彥彊%孫秀巖
맹경남%왕익민%왕온화%류언강%손수암
永磁磁场%人脑微血管内皮细胞%超氧化物歧化酶%丙二醛
永磁磁場%人腦微血管內皮細胞%超氧化物歧化酶%丙二醛
영자자장%인뇌미혈관내피세포%초양화물기화매%병이철
Permanent magnetic fields%Microvascular endothelial brain cells%Superoxide dismutase%Malondialdehyde
目的 观察不同强度永磁磁场对正常和缺氧条件下人脑微血管内皮细胞(HBMEC)氧化损伤的影响.方法 实验分为对照组和8个不同强度(8.1 mT、16.5 mT、20.3 mT、26.0 mT、27.3 mT、62.5 mT、110.7 mT、215.6 mT)的永磁磁场组(加磁1组～加磁8组),在正常和缺氧两种条件体外培养人脑微血管内皮细胞,于72 h后采用超氧化物歧化酶(SOD)法和丙二醛(MDA)法检测各组人脑微血管内皮细胞氧化损伤的变化情况.结果 正常环境下,加磁1组到加磁6组的SOD值均略低于对照组,且加磁7组和加磁8组的SOD值又略高于对照组,但差异均无统计学意义(P＞0.05)；加磁各组的MDA值均高于对照组,其中仅加磁8组的MDA值与对照组比较,差异有统计学意义(P＜0.05).在缺氧环境下,加磁1组、加磁2组、加磁4组、加磁7组、加磁8组的SOD值均低于对照组,而加磁3组、如磁5组、加磁6组的SOD值则高于对照组,但差异均无统计学意义(P＞0.05)；加磁1组、加磁2组、加磁4组的MDA值均高于对照组,加磁3组、加磁5组、加磁6组、加磁7组、加磁8组的MDA值则低于对照组,其中仅加磁7组的MDA值与对照组比较,差异有统计学意义(P＜0.05).结论 正常条件下,磁感强度215.6mT的永磁磁场对人脑微血管内皮细胞MDA有影响；缺氧条件下,磁感强度110.7mT的永磁磁场对人脑微血管内皮细胞MDA有影响.
目的 觀察不同彊度永磁磁場對正常和缺氧條件下人腦微血管內皮細胞(HBMEC)氧化損傷的影響.方法 實驗分為對照組和8箇不同彊度(8.1 mT、16.5 mT、20.3 mT、26.0 mT、27.3 mT、62.5 mT、110.7 mT、215.6 mT)的永磁磁場組(加磁1組～加磁8組),在正常和缺氧兩種條件體外培養人腦微血管內皮細胞,于72 h後採用超氧化物歧化酶(SOD)法和丙二醛(MDA)法檢測各組人腦微血管內皮細胞氧化損傷的變化情況.結果 正常環境下,加磁1組到加磁6組的SOD值均略低于對照組,且加磁7組和加磁8組的SOD值又略高于對照組,但差異均無統計學意義(P＞0.05)；加磁各組的MDA值均高于對照組,其中僅加磁8組的MDA值與對照組比較,差異有統計學意義(P＜0.05).在缺氧環境下,加磁1組、加磁2組、加磁4組、加磁7組、加磁8組的SOD值均低于對照組,而加磁3組、如磁5組、加磁6組的SOD值則高于對照組,但差異均無統計學意義(P＞0.05)；加磁1組、加磁2組、加磁4組的MDA值均高于對照組,加磁3組、加磁5組、加磁6組、加磁7組、加磁8組的MDA值則低于對照組,其中僅加磁7組的MDA值與對照組比較,差異有統計學意義(P＜0.05).結論 正常條件下,磁感彊度215.6mT的永磁磁場對人腦微血管內皮細胞MDA有影響；缺氧條件下,磁感彊度110.7mT的永磁磁場對人腦微血管內皮細胞MDA有影響.
목적 관찰불동강도영자자장대정상화결양조건하인뇌미혈관내피세포(HBMEC)양화손상적영향.방법 실험분위대조조화8개불동강도(8.1 mT、16.5 mT、20.3 mT、26.0 mT、27.3 mT、62.5 mT、110.7 mT、215.6 mT)적영자자장조(가자1조～가자8조),재정상화결양량충조건체외배양인뇌미혈관내피세포,우72 h후채용초양화물기화매(SOD)법화병이철(MDA)법검측각조인뇌미혈관내피세포양화손상적변화정황.결과 정상배경하,가자1조도가자6조적SOD치균략저우대조조,차가자7조화가자8조적SOD치우략고우대조조,단차이균무통계학의의(P＞0.05)；가자각조적MDA치균고우대조조,기중부가자8조적MDA치여대조조비교,차이유통계학의의(P＜0.05).재결양배경하,가자1조、가자2조、가자4조、가자7조、가자8조적SOD치균저우대조조,이가자3조、여자5조、가자6조적SOD치칙고우대조조,단차이균무통계학의의(P＞0.05)；가자1조、가자2조、가자4조적MDA치균고우대조조,가자3조、가자5조、가자6조、가자7조、가자8조적MDA치칙저우대조조,기중부가자7조적MDA치여대조조비교,차이유통계학의의(P＜0.05).결론 정상조건하,자감강도215.6mT적영자자장대인뇌미혈관내피세포MDA유영향；결양조건하,자감강도110.7mT적영자자장대인뇌미혈관내피세포MDA유영향.
Objective To observe the effects of permanent magnetic fields with different magnetic inductions on oxidative damage to human brain microvascular endothelial cells (HBMECs) under normal and hypoxic conditions. Methods HBMECs were cultured in vitro under normal and hypoxic conditions,then divided into a control group and groups receiving magnetic induction at 8.1,16.5,20.3,26.0,27.3,62.5,110.7 and 215.6 mT.Changes in superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in each group were measured 72 h after exposure to the magnetic fields. Results Under normal conditions the SOD activities of the magnetic groups were not significantly different from that of the control group. Only the MDA content of the 215.6 mT group was statistically different (slightly higher) than that of the control group (P ＜ 0.05 ).Under hypoxic conditions,there was again no statistically significant difference in SOD activity,but the MDA content of the 110.7 mT group was significantly lower. Conclusions Under normal conditions,a 215.6 mT permanent magnetic field has an effect on MDA in HBMECs; under hypoxic conditions a 110.7 mT permanent magnetic field has such an effect.
