车前子对抗高脂血症大鼠脂质过氧化作用
차전자대항고지혈증대서지질과양화작용
Effect of plantain seed on the lipid peroxidation in rats with hyperlipidemia
  • 万方数据
    中国临床康复 중국림상강복
    CHINESE JOURNAL OF CLINICAL REHABILITATION
    2006年 19期 184-186 ,共3页

    王素敏%杨更亮%代洪燕%裴庭梅%王永利

    왕소민%양경량%대홍연%배정매%왕영리

    车前子%高脂血症/中药疗法%脂质过氧化作用 車前子%高脂血癥/中藥療法%脂質過氧化作用
    차전자%고지혈증/중약요법%지질과양화작용
    背景:近年研究发现,车前草种子外壳是很好的可溶性膳食纤维源,可用来添加到食品中,调整胆固醇含量.目的:观察车前子对高脂血症大鼠血脂及其体内脂质过氧化作用的干预结果.设计:完全随机分组设计,对照动物实验.单位:河北省新药药理毒理实验室.材料:①选用健康一级SD大鼠24只,鼠龄60～70 d,体质量(210±22)g,雌雄不拘.②基础饲料由河北省实验动物中心配制,各成分质量分数:面粉0.25,麸皮0.1,玉米面0.22,豆饼0.22,鱼粉0.08,骨粉0.02,草粉0.05,食盐0.01,酵母粉0.02,葵花子0.03.高脂饲料由河北省实验动物中心配制,各成分质量分数:基础饲料0.9,胆固醇0.015,猪油0.08,猪胆盐0.003.③测定血脂试剂盒由保定长城临床试剂公司出品;测超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活力和丙二醛水平试剂盒由南京建成生物工程研究所提供.方法:实验于2004-06/12在河北省新药药理毒理研究实验室完成.①随机将24只大鼠分为3组:正常对照组,模型组,车前子组,每组8只.正常对照组:进食基础饲料.模型组:进食高脂饲料.车前子组:进食高脂饲料+车前子15 g/kg,普通饮水.实验动物分笼饲养,每只摄食25 g/d,自由饮水,实验周期为12周.②实验结束时,麻醉大鼠,采用相应试剂盒测定血清三酰甘油、总胆固醇、高密度脂蛋白胆固醇水平;采用相应试剂盒测血清超氧化物歧化酶、过氧化氢酶活力和丙二醛水平,心肌组织超氧化物歧化酶活力及丙二醛含量,肝组织过氧化氢酶和谷胱甘肽过氧化物酶活力.③两组间计量资料差异比较采用t检验.主要观察指标:造模12周后,各组大鼠血脂水平及机体抗氧化能力比较.结果:大鼠24只均进入结果分析.①造模12周后,模型组大鼠血清和心肌组织超氧化物歧化酶活力明显低于正常对照组和车前子组(P＜0.05),血清丙二醛水平和心肌丙二醛含量均明显高于正常对照组和车前子组(P＜0.05).②造模12周后,模型组大鼠血清和心肌组织过氧化氢酶和肝组织谷胱甘肽过氧化物酶活力均明显低于正常对照组和车前子组(P＜0.05).③造模12周后,模型组大鼠血清总胆固醇和三酰甘油水平明显高于正常对照组和车前子组(P＜0.05),血清高密度脂蛋白胆固醇水平和高密度脂蛋白胆固醇/总胆固醇明显低于正常对照组和车前子组(P＜0.05).结论:提示15g/kg剂量车前子可明显降低高脂血症大鼠血脂,增加机体抗氧化能力.
    배경:근년연구발현,차전초충자외각시흔호적가용성선식섬유원,가용래첨가도식품중,조정담고순함량.목적:관찰차전자대고지혈증대서혈지급기체내지질과양화작용적간예결과.설계:완전수궤분조설계,대조동물실험.단위:하북성신약약리독리실험실.재료:①선용건강일급SD대서24지,서령60～70 d,체질량(210±22)g,자웅불구.②기출사료유하북성실험동물중심배제,각성분질량분수:면분0.25,부피0.1,옥미면0.22,두병0.22,어분0.08,골분0.02,초분0.05,식염0.01,효모분0.02,규화자0.03.고지사료유하북성실험동물중심배제,각성분질량분수:기출사료0.9,담고순0.015,저유0.08,저담염0.003.③측정혈지시제합유보정장성림상시제공사출품;측초양화물기화매、과양화경매、곡광감태과양화물매활력화병이철수평시제합유남경건성생물공정연구소제공.방법:실험우2004-06/12재하북성신약약리독리연구실험실완성.①수궤장24지대서분위3조:정상대조조,모형조,차전자조,매조8지.정상대조조:진식기출사료.모형조:진식고지사료.차전자조:진식고지사료+차전자15 g/kg,보통음수.실험동물분롱사양,매지섭식25 g/d,자유음수,실험주기위12주.②실험결속시,마취대서,채용상응시제합측정혈청삼선감유、총담고순、고밀도지단백담고순수평;채용상응시제합측혈청초양화물기화매、과양화경매활력화병이철수평,심기조직초양화물기화매활력급병이철함량,간조직과양화경매화곡광감태과양화물매활력.③량조간계량자료차이비교채용t검험.주요관찰지표:조모12주후,각조대서혈지수평급궤체항양화능력비교.결과:대서24지균진입결과분석.①조모12주후,모형조대서혈청화심기조직초양화물기화매활력명현저우정상대조조화차전자조(P＜0.05),혈청병이철수평화심기병이철함량균명현고우정상대조조화차전자조(P＜0.05).②조모12주후,모형조대서혈청화심기조직과양화경매화간조직곡광감태과양화물매활력균명현저우정상대조조화차전자조(P＜0.05).③조모12주후,모형조대서혈청총담고순화삼선감유수평명현고우정상대조조화차전자조(P＜0.05),혈청고밀도지단백담고순수평화고밀도지단백담고순/총담고순명현저우정상대조조화차전자조(P＜0.05).결론:제시15g/kg제량차전자가명현강저고지혈증대서혈지,증가궤체항양화능력.
    BACKGROUND: Recently, it is investigated that shell of plantain seed is a soluble dietary fiber which can be added into foods to regulate content of cholesterol.OBJECTIVE: To investigate the interventional effect of plantain seed on lipid and its lipid peroxidation in rats with hyperlipidemia.DESIGN: Completely randomized grouping design and controlled animal study.SETTING: Laboratory of Pharmacology and Toxicology for New Drug in Hebei Province.MATERIALS: ① A total of 24 healthy SD rats, of grade I, aged 60-70 days, weighting (210±22) g, of either gender, were selected in this study. ② Basic feed was provided by Experimental Animal Center in Hebei Province,and the fractional mass of each component was mentioned as following:flour 0.25, bran 0.1, corn dust 0.22, bean cake 0.22, fish dust 0.02, bone dust 0.02, grass dust 0.05, salt 0.01, yeast dust 0.02, and sunflower seed 0.03. High fat feed was provided by Experimental Animal Center in Hebei Province, and the fractional mass of each component was mentioned as following: basic 0.9, cholesterol 0.015, lard 0.08, and hyocholic salt 0.003.③ Lipid kit was provided by Baoding Changcheng Clinical Reagent Company, and kits of superoxide dismutase (SOD), catalase (CAT),glutathione peroxidase (GSH-Px) and malondialdehyde (MDA) were provided by Nanjing Jiancheng Bioengineering Institute.METHODS: The experiment was completed at the Laboratory of Pharmacology and Toxicology for New Drug in Hebei Province from June to December 2004. ① All 24 rats were randomly divided into 3 groups:normal control group, model group and plantain seed group with 8 in each group. Rats in the normal control group were fed with basic feed. Rats in plantain seed group were fed with high fat feed + 15 g/kg plantain seed and drank routinely. Experimental rats were fed in cages, respectively.Each one was fed with 25 g/d food and drunk freely. The experimental cycle was 12 weeks. ② At the end of experiment, rats were anesthetized to assayed levels of serum triacylglycerol (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C), serum SOD and MDA, activities of CAT and SOD in myocardial tissue, content of MDA, and activities of CAT and GSH-Px in hepatic tissue with related kits. ③ Measurement data were compared between each two group with t test.MAIN OUTCOME MEASURES:Comparison of serum lipid level and anti-oxidation among groups at 12 weeks after modeling.RESULTS: All 24 rats were involved in the final analysis. ① At 12 weeks after modeling, activities of SOD in serum and myocardial tissue were lower in model group than those in normal control group and plantain seed group (P ＜ 0.05), but levels of MDA in serum and myocardial tissue were higher in model group than those in normal control group and plantain seed group (P ＜ 0.05). ② At 12weeks after modeling, activities of CAT and GSH-Px in serum and myocardial tissue were lower in model group than those in normal control group and plantain seed group (P ＜ 0.05). ③ At 12 weeks after modeling, levels of TC and TG in serum were higher in model group than those in normal control group and plantain seed group (P ＜ 0.05), but level of HDL-C and ratio between HDL-C and TC in serum were lower in model group than those in normal control group and plantain seed group (P ＜ 0.05).CONCLUSION: Plantain seed at dosage of 15 g/kg can decrease content of lipid and strengthen anti-oxidation of economy in rats with hyperlipidemia.
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