CAJ | 학술논문

目的:探讨乳腺癌细胞系MCF-7和MDA-MB-231中是否存在乳腺癌起始细胞(CD44+/CD24-/low)亚群;并分析Hedgehog信号通路在CD44+/CD24-/low乳腺癌起始细胞亚群中的表达.方法:应用荧光激活细胞分选方法检测与分选MCF-7和MDA-MB-231中的CD44+/CD24-/low细胞亚群和非CD44+/CD24-/low细胞亚群,并在激光共聚焦显微镜下进行观察确认.应用逆转录聚合酶链反应(RT-PCR)方法检测CD44+/CD24-/low细胞亚群和非CD44+/CD24-/low细胞亚群中Hedgehog信号通路重要基因PTCH(human patched gene)、SHH(sonic Hedgehog)、Gii-1(glioma-associated oncogene homoglog-1)和SMOH(smoothened homolog)的表达情况.结果:乳腺癌细胞系MCF-7和MDA-MB-231中CD44+/CD24-/low亚群,比例分别为(1.70±1.43)%和(94.2±1.2)%.乳腺癌细胞系MCF-7和MDA-MB-231的CD44+/CD24-/low亚群中Hedgehog信号通路处于明显活化状态,其中Hedgehog信号通路中重要下游信号分子Gii-1在MCF-7和MDA-MB-231细胞系CD44+/CD24-/low细胞亚群中的表达要明显高于非CD44+/CD24-/low细胞亚群(P分别为0.007和0.005).结论:乳腺癌细胞系MCF-7和MDA-MB-231中存在CD44+/CD24-/low干细胞标志细胞亚群,其中Hedgehog信号通路处于明显活化状态.
목적:탐토유선암세포계MCF-7화MDA-MB-231중시부존재유선암기시세포(CD44+/CD24-/low)아군;병분석Hedgehog신호통로재CD44+/CD24-/low유선암기시세포아군중적표체.방법:응용형광격활세포분선방법검측여분선MCF-7화MDA-MB-231중적CD44+/CD24-/low세포아군화비CD44+/CD24-/low세포아군,병재격광공취초현미경하진행관찰학인.응용역전록취합매련반응(RT-PCR)방법검측CD44+/CD24-/low세포아군화비CD44+/CD24-/low세포아군중Hedgehog신호통로중요기인PTCH(human patched gene)、SHH(sonic Hedgehog)、Gii-1(glioma-associated oncogene homoglog-1)화SMOH(smoothened homolog)적표체정황.결과:유선암세포계MCF-7화MDA-MB-231중CD44+/CD24-/low아군,비례분별위(1.70±1.43)%화(94.2±1.2)%.유선암세포계MCF-7화MDA-MB-231적CD44+/CD24-/low아군중Hedgehog신호통로처우명현활화상태,기중Hedgehog신호통로중중요하유신호분자Gii-1재MCF-7화MDA-MB-231세포계CD44+/CD24-/low세포아군중적표체요명현고우비CD44+/CD24-/low세포아군(P분별위0.007화0.005).결론:유선암세포계MCF-7화MDA-MB-231중존재CD44+/CD24-/low간세포표지세포아군,기중Hedgehog신호통로처우명현활화상태.