CAJ | 학술논문

[目的]通过检测猪瘟疫苗免疫后的IFN-γ水平探讨仔猪细胞免疫情况.[方法]采用超前免疫和普通免疫2种方法对仔猪进行猪瘟免疫,用流式细胞仪对猪瘟疫苗免疫后仔猪外周血单核细胞(PBMC)中分泌IFN-γ细胞数量进行检测. [结果]无论采取哪一种方法进行免疫,仔猪PBMC中分泌IFN-γ细胞的比例均显著增多,且一直维持在较高的水平.当在60日龄仔猪进行第2次免疫后分泌时,IFN-γ细胞的比例增加更为明显,而对照组仔猪分泌IFN-γ细胞的比例在试验过程中则一直维持较低的水平,这说明猪瘟疫苗能够诱导仔猪体内IFN-γ水平升高,间接表明猪瘟疫苗注射后激发了机体的细胞免疫应答.[结论]与普通免疫方法相比,采用超前免疫方法能够使仔猪及早地产生针对猪瘟的细胞免疫反应.
[목적]통과검측저온역묘면역후적IFN-γ수평탐토자저세포면역정황.[방법]채용초전면역화보통면역2충방법대자저진행저온면역,용류식세포의대저온역묘면역후자저외주혈단핵세포(PBMC)중분비IFN-γ세포수량진행검측. [결과]무론채취나일충방법진행면역,자저PBMC중분비IFN-γ세포적비례균현저증다,차일직유지재교고적수평.당재60일령자저진행제2차면역후분비시,IFN-γ세포적비례증가경위명현,이대조조자저분비IFN-γ세포적비례재시험과정중칙일직유지교저적수평,저설명저온역묘능구유도자저체내IFN-γ수평승고,간접표명저온역묘주사후격발료궤체적세포면역응답.[결론]여보통면역방법상비,채용초전면역방법능구사자저급조지산생침대저온적세포면역반응.
[Objective] This study aimed to discuss the immunization of the cell in piglets immunized with classical swine fever vaccine through detection on IFN-γ Level in immunized piglets.[Method] Piglets were immunized with classical swine fever (CSF) vaccine by common immunization and ultra Pre-immunity and the cells secreting IFN-γ in peripheral blood mononuclear cells (PBMC) were detected by flow cytometry after piglet immunization.[Result] The proportion of cells secreting IFN-γ in PBMC was increased significantly and retained a high level no matter which immunity method was adopted and that was increased more significantly after the secondary immunization for piglets at 60 days,while that in CK group retained a lower level during the whole process of experiment,indicating that the immunization of CSF vaccine could induce the rising of level of IFN-γ in piglets and indirectly indicating that the cellular immunity was provoked after the injection of CSF vaccine.[Conclusion] Compared with the method of common immunization,the ultra pre-immunity method could made the piglets early produce the cell immunoreaction aiming to CSF.