CAJ | 학술논문

利用真菌β-微管蛋白基因的保守序列和小麦条锈菌(Puccinia striiformi f.sp.tritici)、叶锈菌(Puccinia tri-ticina)的SCAR标记引物,对锈菌孢子与感病小麦叶片总DNA进行复合PCR检测,扩增获得了小麦条锈菌171bp和叶锈菌226 bp的特异性DNA片段,其检测灵敏度可达到50 μg·L~(-1)模板DNA浓度水平.在此基础上,可进一步制备小麦锈菌不同种的分子诊断、检测试剂盒.
이용진균β-미관단백기인적보수서렬화소맥조수균(Puccinia striiformi f.sp.tritici)、협수균(Puccinia tri-ticina)적SCAR표기인물,대수균포자여감병소맥협편총DNA진행복합PCR검측,확증획득료소맥조수균171bp화협수균226 bp적특이성DNA편단,기검측령민도가체도50 μg·L~(-1)모판DNA농도수평.재차기출상,가진일보제비소맥수균불동충적분자진단、검측시제합.
In this research,a multiplex PCR analysis system was built successfully.The 171 bp spe-cific DNA fragment of Puccinia striiformis f.sp.tritici and the 226 bp specific DNA fragment of Puc-cinia triticina were amplified from the corresponding urediospores and the infected wheat leaves.The detection sensitivity of this multiplex PCR was 50μg·L~(-1) template DNA.On this basis,a high-through kit can be further developed for the rapid diagnosis and detection of wheat rust fungi.