CAJ | 학술논문

通过富集培养技术结合高效液相色谱法检测,从长期受丁草胺污染的污泥中筛选出1株丁草胺降解菌,命名为Y-1.经形态特征、生理生化特征和16S rDNA序列同源性分析,将该菌株鉴定为假单胞菌属(Pseudomonas sp.).通过研究Y-1在不同条件下的降解特性发现,降解丁草胺的最优条件为:丁草胺初始浓度20 mg/L、接种量5％、pH值7、培养温度30℃.在最优环境条件下培养7d,Y-1能降解培养液中76％的丁草胺,显示了良好的降解能力.
통과부집배양기술결합고효액상색보법검측,종장기수정초알오염적오니중사선출1주정초알강해균,명명위Y-1.경형태특정、생리생화특정화16S rDNA서렬동원성분석,장해균주감정위가단포균속(Pseudomonas sp.).통과연구Y-1재불동조건하적강해특성발현,강해정초알적최우조건위:정초알초시농도20 mg/L、접충량5％、pH치7、배양온도30℃.재최우배경조건하배양7d,Y-1능강해배양액중76％적정초알,현시료량호적강해능력.
From the sludge polluted by butachlor for a long time,the degrading bacterium to butachlor was selected through enrichment culture with combination of High Performance Liquid Chromatography (HPLC) detection and then named as Y-1.Through the analysis of morphological characteristics,physiological and biochemical characteristics,and 16S rDNA sequence homology,Y-1 was identified as Pseudomonas sp..Through the research of the degradation character of Y-1 in different ambient conditions,the optimal conditions were found as follows:the initial concentration of butachlor of 20 mg/L,inoculum concentration of 5％,pH of 7 and temperature of 30℃.The degradation rate of Y-1 to butachlor reached 76％ after the cultivation for 7 days in optimal conditions,which showed the excellent degradation ability.