CAJ | 학술논문

目的探讨血管内介入技术建立肠系膜上静脉-门静脉血栓动物模型的可行性.方法小型猪9头,选用1头行预实验,8头用于正式实验.全麻下经皮经肝门静脉穿刺,用球囊导管阻塞门静脉主干并注入凝血酶或自体血栓,30 min后行门静脉造影证实肠系膜上静脉-门静脉血栓形成,并比较血栓形成前后影像学变化.对术中死亡动物进行解剖,分析死亡原因,必要时取组织行病理学检查.结果 9头猪均成功建立肠系膜上静脉-门静脉血栓模型.第1头预实验猪模型建立后10 min死亡,病理证实死亡原因为DIC.正式实验8头猪,6头肠系膜上静脉-门静脉血栓形成后饲养14 d,其余2头术后3 h内死亡,死亡原因分别为肝破裂和麻醉药物过量.结论应用血管内介入技术可以建立肠系膜上静脉-门静脉血栓动物模型,为肠系膜上静脉-门静脉血栓的相关研究提供实验基础.
목적 탐토혈관내개입기술건립장계막상정맥-문정맥혈전동물모형적가행성.방법 소형저9두,선용1두행예실험,8두용우정식실험.전마하경피경간문정맥천자,용구낭도관조새문정맥주간병주입응혈매혹자체혈전,30 min후행문정맥조영증실장계막상정맥-문정맥혈전형성,병비교혈전형성전후영상학변화.대술중사망동물진행해부,분석사망원인,필요시취조직행병이학검사.결과 9두저균성공건립장계막상정맥-문정맥혈전모형.제1두예실험저모형건립후10 min사망,병리증실사망원인위DIC.정식실험8두저,6두장계막상정맥-문정맥혈전형성후사양14 d,기여2두술후3 h내사망,사망원인분별위간파렬화마취약물과량.결론 응용혈관내개입기술가이건립장계막상정맥-문정맥혈전동물모형,위장계막상정맥-문정맥혈전적상관연구제공실험기출.
Objective To assess the feasibility of interventional techniques in the establishment of animal model of superior mesenteric vein-portal vein (SMV-PV) thrombosis. Methods Nine miniature pigs were involved in the study including one for preliminary experiment. After general anesthesia, a balloon catheter was placed in the main trunk of PV to block the portal flow and then thrombin or autologous blood clot was injected to the SMV. Venography was performed to confirm the thrombosis 30 minutes later. Changes in the imaging before and after the thrombosis were observed. Pigs died during the experiment were anatomized to analyze the causes, and pathological examination was performed when necessary. Results The model of SMV-PV thrombosis was successfully established in all the pigs. One pig died of diffuse intravascular coagulation 10 minutes after model establishment in the preliminary experiment. Two pigs died of hepatorrhexis and over dose of anesthetics respec-tively 3 hours after model establishment, and the rest 6 pigs were fed for 14 days. Conclusion Interventional techniques are effective in the establishment of SMV-PV thrombosis model.