CAJ | 학술논문

目的探讨直肠癌组织耐药相关蛋白的表达及川芎嗪联合化疗的意义.方法从耐药直肠癌患者手术切除直肠癌组织上分离制备细胞悬液,并利用流式细胞仪和Western-Bitting研究P-170、多药耐药相关蛋白(Muhidrug resistance protein,MRP)、肺耐药相关蛋白(Lung re-sistance associated protein,LRP)等耐药蛋白的表达情况,以阐明耐药直肠癌细胞的耐药机理,另外应用中药川芎嗪对5-FU进行增敏实验,再联合5-FU对耐药直肠癌患者进行化疗,并与常规化疗进行疗效比较.结果耐药直肠癌细胞的耐药机理主要是通过上调P-170、MRP等耐药蛋白的表达实现,而中药川芎嗪则可以通过下调P-170、MRP等耐药蛋白的表达米增敏耐药直肠癌细胞.结论川芎嗪可以增加耐药直肠癌细胞对化疗药物的敏感性,川芎嗪联合化疗可以有效增加耐药患者的近期有效率,值得临床推广.-sistance associated protein,LRP)等耐药蛋白的表达情况,以阐明耐药直肠癌细胞的耐药机理,另外应用中药川芎嗪对5-FU进行增敏实验,再联合5-FU对耐药直肠癌患者进行化疗,并与常规化进行疗效比较.结果耐药直肠癌细胞的耐药机理主要是通过上调p-170、MRP等耐药蛋白的表达实现,而中药川芎嗪则可以通过下调P-170、MRP等耐药蛋白的表达米增敏耐药直肠癌细胞.结论川芎嗪可以增加耐药直肠癌细胞对化疗药物的敏感性,川芎嗪联合化疗可以有效增加耐药患者的近期有效率,值得临床推广.-sistance asso
목적 탐토직장암조직내약상관단백적표체급천궁진연합화료적의의.방법 종내약직장암환자수술절제직장암조직상분리제비세포현액,병이용류식세포의화Western-Bitting연구P-170、다약내약상관단백(Muhidrug resistance protein,MRP)、폐내약상관단백(Lung re-sistance associated protein,LRP)등내약단백적표체정황,이천명내약직장암세포적내약궤리,령외응용중약천궁진대5-FU진행증민실험,재연합5-FU대내약직장암환자진행화료,병여상규화료진행료효비교.결과 내약직장암세포적내약궤리주요시통과상조P-170、MRP등내약단백적표체실현,이중약천궁진칙가이통과하조P-170、MRP등내약단백적표체미증민내약직장암세포.결론 천궁진가이증가내약직장암세포대화료약물적민감성,천궁진연합화료가이유효증가내약환자적근기유효솔,치득림상추엄.-sistance associated protein,LRP)등내약단백적표체정황,이천명내약직장암세포적내약궤리,령외응용중약천궁진대5-FU진행증민실험,재연합5-FU대내약직장암환자진행화료,병여상규화진행료효비교.결과 내약직장암세포적내약궤리주요시통과상조p-170、MRP등내약단백적표체실현,이중약천궁진칙가이통과하조P-170、MRP등내약단백적표체미증민내약직장암세포.결론 천궁진가이증가내약직장암세포대화료약물적민감성,천궁진연합화료가이유효증가내약환자적근기유효솔,치득림상추엄.-sistance asso
Objective To investigate resistance-related protein in tumor tissue expression and significance of combination chemotherapy with Liguslrazine.Methods In this paper, drug-resistant cancer patients resected tumor tissue was isolated preparation of cell suspension. Study of P-170, MRP and LRP by using flow cytometry and Western-Blotting to elucidate the mechanism of drug resistance, and use traditional Chinese medicine Ligustrazine reversal of multidrug resistance experiments.Results LOVO/5-FU resistance in the mechanism of drug-resistant mainly by increasing P-170, MRP protein expression. Chinese medicine Ligustrazine can be adopted to reduce P-170, MRP protein expression to sensitize drug-resistant rectal cancer cells.Conclusions Ligustrazine can increase the resistance of cancer cells to chemotherapeutic drug sensitivity, then the Ligustrazine can effectively increase the ORR in patients with short-term effect, worthy to be popularized.