中华老年医学杂志
中華老年醫學雜誌
중화노년의학잡지
Chinese Journal of Geriatrics
2012年
9期
810-813
,共4页
李云桥%侯晓华%王艺峰%张俐娜
李雲橋%侯曉華%王藝峰%張俐娜
리운교%후효화%왕예봉%장리나
茯苓%葡聚糖类%胃肿瘤
茯苓%葡聚糖類%胃腫瘤
복령%포취당류%위종류
Poria cocos%Glucans%Stomach neoplasms
目的 探讨茯苓多糖及其衍生物结构与抗老年大鼠胃癌活性之间的构效关系. 方法 从茯苓菌核中提取的(1→3)β-D-葡聚糖(PCS3-Ⅱ)及其硫酸酯、羧甲基、甲基化、羟乙基、羟丙基衍生物,观察PCS3-Ⅱ及5种衍生物在体内外对胃癌细胞株MKN-45、SGC 7901和MKN-28生长的抑制作用. 结果 未改性β-葡聚糖PCS3-Ⅱ几乎无抗胃癌活性,而PCS3-Ⅱ硫酸酯和羧甲基衍生物对细胞株MKN-45、SGC-7901、MKN-28有较高的抑制率；体内实验显示,硫酸酯和羧甲基衍生物对老年大鼠MKN-45胃癌移植瘤亦有较高抑制率,剂量为100 mg/kg时对老年大鼠胃肿瘤的抑制率分别达到32.7％和36.4％. 结论 天然茯苓菌核多糖体外无抗胃癌活性；PCS3-Ⅱ羧甲基和硫酸酯基后衍生物溶于水,其抗胃癌活性增强；良好的水溶性、较高的链刚性和适当大的分子量有利于提高茯苓多糖抗老年人胃癌的活性.
目的 探討茯苓多糖及其衍生物結構與抗老年大鼠胃癌活性之間的構效關繫. 方法 從茯苓菌覈中提取的(1→3)β-D-葡聚糖(PCS3-Ⅱ)及其硫痠酯、羧甲基、甲基化、羥乙基、羥丙基衍生物,觀察PCS3-Ⅱ及5種衍生物在體內外對胃癌細胞株MKN-45、SGC 7901和MKN-28生長的抑製作用. 結果 未改性β-葡聚糖PCS3-Ⅱ幾乎無抗胃癌活性,而PCS3-Ⅱ硫痠酯和羧甲基衍生物對細胞株MKN-45、SGC-7901、MKN-28有較高的抑製率；體內實驗顯示,硫痠酯和羧甲基衍生物對老年大鼠MKN-45胃癌移植瘤亦有較高抑製率,劑量為100 mg/kg時對老年大鼠胃腫瘤的抑製率分彆達到32.7％和36.4％. 結論 天然茯苓菌覈多糖體外無抗胃癌活性；PCS3-Ⅱ羧甲基和硫痠酯基後衍生物溶于水,其抗胃癌活性增彊；良好的水溶性、較高的鏈剛性和適噹大的分子量有利于提高茯苓多糖抗老年人胃癌的活性.
목적 탐토복령다당급기연생물결구여항노년대서위암활성지간적구효관계. 방법 종복령균핵중제취적(1→3)β-D-포취당(PCS3-Ⅱ)급기류산지、최갑기、갑기화、간을기、간병기연생물,관찰PCS3-Ⅱ급5충연생물재체내외대위암세포주MKN-45、SGC 7901화MKN-28생장적억제작용. 결과 미개성β-포취당PCS3-Ⅱ궤호무항위암활성,이PCS3-Ⅱ류산지화최갑기연생물대세포주MKN-45、SGC-7901、MKN-28유교고적억제솔；체내실험현시,류산지화최갑기연생물대노년대서MKN-45위암이식류역유교고억제솔,제량위100 mg/kg시대노년대서위종류적억제솔분별체도32.7％화36.4％. 결론 천연복령균핵다당체외무항위암활성；PCS3-Ⅱ최갑기화류산지기후연생물용우수,기항위암활성증강；량호적수용성、교고적련강성화괄당대적분자량유리우제고복령다당항노년인위암적활성.
Objective To study the correlation between structure of b D -glucan derivatives from Poria cocos sclerotiuma and anti gastric cancer activities of elderly rats.Methods A water insoluble (1→3)-β-D-glucan (PCS3-Ⅱ ) isolated from fresh sclerotium of Poria cocos was sulfated,carboxymethylated,methylated,hydroxyethylated and hydroxypropylated,respectively,to prepare five water-soluble derivatives.Their activities of the native β-glucan and five derivatives against gastric cancer cell strains of MKN-45,SGC 7901and MKN-28 were tested in vitro and in vivo.Results The native β-glucan did not show any anti-gastric cancer activity,while the sulfated and carboxymethylated derivatives significantly exhibited the anti gastric cancer activity against MKN-45,SGC-7901 and MKN-28 cells in vitro,and elderly rats with MKN-45 transplanted tumor,especially.The gastric cancer inhibition rates of elderly rats were 32.7％ and 36.4％ for the 100 mg/kg sulfated and carboxymethylated derivatives,respectively.Conclusions The fresh sclerotium of Poria cocos polysaccharides can not show any anti gastric cancer activity in vitro,but the sulfated and carboxymethylated derivatives may increase the inhibition effect.Water-solubility,higher chain stiffness and relatively molecular weight would be benefit to increase anti-elderly gastric cancer activity of polysaccharides from Poria cocos sclerotiuma.
