中华核医学杂志
中華覈醫學雜誌
중화핵의학잡지
CHINESE JOURNAL OF NUCLEAR MEDICINE
2009年
2期
99-102
,共4页
多巴胺%甲状腺功能亢进症%大鼠%TRODAT.1
多巴胺%甲狀腺功能亢進癥%大鼠%TRODAT.1
다파알%갑상선공능항진증%대서%TRODAT.1
Dopamine%Hyperparathyroidism%Rats%TRODAT-1
目的 研究甲状腺激素升高时脑纹状体多巴胺转运蛋白(DAT)、多巴胺(DA)及其代谢产物3,4二羟苯乙酸(DOPAC)的含量变化.方法 将24只大鼠分为2组,其中一组采用灌胃法连续每天给予大鼠左甲状腺素钠(优甲乐,用量按大鼠体质量25 ug100 g,溶于生理盐水中,体积1ml造成实验性大鼠甲状腺激素增高状态;对照组仅采用灌胃法给予生理盐水1 ml;于模型建立前后按随机数字表法抽取左甲状腺素钠组及对照组大鼠各6只,分别取尾静脉血1 ml,采用放射免疫法测定TT3及TT4水平.14 d后将上述对照组及左甲状腺素钠组大鼠再各自平均分为2组,一组用于99Tcm-2B-[N,N'一双(2-巯乙基)乙撑二胺基]甲基-3B-(4-氯苯基)托烷(TRODAT-1)大鼠脑内分布研究,另一组用于高效液相一电化学检测器(HPLC-ECD)测纹状体DA及DOPAC含量,观察上述大鼠脑纹状体区DAT的分布及DA、DOPAC含量的变化.多组比较采用ANOVA分析,2组间比较采用均数t检验.结果 左甲状腺素钠组大鼠在服用左甲状腺素钠后活动明显增加,体质量增长低于正常对照组[(223.90±8.40)与(261.60±14.20)g,t=6.98,P<0.05].甲状腺激素水平测定示:左甲状腺素钠组给药前后TT3分别为(1.46±0.17)和(2.72±O.29)nmol/L(t=10.51.P<O.0001);TT4分别为(83.52±10.06)和(187.12±36.48)nmol/L(t=7.74,P<0.0001);对照组生理盐水注射前后,TT3分别为(I.54±O.09)和(I.71±0.20)mot/L(t=1.68,P>0.05),TT4分别为(98.38±9.77)和(88.38±6.76)mnel/L(t=1.36,P>0.05).99Tcm-TRODAT-I大鼠脑内分布(每克脑组织百分注射剂量率,%ID/g)研究示:左甲状腺素钠组纹状体为(2.80±O.25)%ID/g,明显低于正常对照组[(3.13±O.14)%ID/g,t=一3.62,P<0.05].经检测左甲状腺素钠组脑纹状体DA含量为(12 109.30±629.20),ng/g脑组织,明显高于对照组[(9012.CO±318.80)ng/g脑组织,t=10.76,P<O.0001];DOPAC含量为(1079.30+97.00)ng/g脑组织,亦高于对照组[(873.20±67.50)ng/g脑组织,t:4.27,P<0.05].结论 在实验性甲状腺功能亢进症(简称甲亢)时,脑纹状体DA及其代谢产物的合成及代谢加快,与99Tcm-TRODAT-1结合的DAT水平则出现下降趋势.
目的 研究甲狀腺激素升高時腦紋狀體多巴胺轉運蛋白(DAT)、多巴胺(DA)及其代謝產物3,4二羥苯乙痠(DOPAC)的含量變化.方法 將24隻大鼠分為2組,其中一組採用灌胃法連續每天給予大鼠左甲狀腺素鈉(優甲樂,用量按大鼠體質量25 ug100 g,溶于生理鹽水中,體積1ml造成實驗性大鼠甲狀腺激素增高狀態;對照組僅採用灌胃法給予生理鹽水1 ml;于模型建立前後按隨機數字錶法抽取左甲狀腺素鈉組及對照組大鼠各6隻,分彆取尾靜脈血1 ml,採用放射免疫法測定TT3及TT4水平.14 d後將上述對照組及左甲狀腺素鈉組大鼠再各自平均分為2組,一組用于99Tcm-2B-[N,N'一雙(2-巰乙基)乙撐二胺基]甲基-3B-(4-氯苯基)託烷(TRODAT-1)大鼠腦內分佈研究,另一組用于高效液相一電化學檢測器(HPLC-ECD)測紋狀體DA及DOPAC含量,觀察上述大鼠腦紋狀體區DAT的分佈及DA、DOPAC含量的變化.多組比較採用ANOVA分析,2組間比較採用均數t檢驗.結果 左甲狀腺素鈉組大鼠在服用左甲狀腺素鈉後活動明顯增加,體質量增長低于正常對照組[(223.90±8.40)與(261.60±14.20)g,t=6.98,P<0.05].甲狀腺激素水平測定示:左甲狀腺素鈉組給藥前後TT3分彆為(1.46±0.17)和(2.72±O.29)nmol/L(t=10.51.P<O.0001);TT4分彆為(83.52±10.06)和(187.12±36.48)nmol/L(t=7.74,P<0.0001);對照組生理鹽水註射前後,TT3分彆為(I.54±O.09)和(I.71±0.20)mot/L(t=1.68,P>0.05),TT4分彆為(98.38±9.77)和(88.38±6.76)mnel/L(t=1.36,P>0.05).99Tcm-TRODAT-I大鼠腦內分佈(每剋腦組織百分註射劑量率,%ID/g)研究示:左甲狀腺素鈉組紋狀體為(2.80±O.25)%ID/g,明顯低于正常對照組[(3.13±O.14)%ID/g,t=一3.62,P<0.05].經檢測左甲狀腺素鈉組腦紋狀體DA含量為(12 109.30±629.20),ng/g腦組織,明顯高于對照組[(9012.CO±318.80)ng/g腦組織,t=10.76,P<O.0001];DOPAC含量為(1079.30+97.00)ng/g腦組織,亦高于對照組[(873.20±67.50)ng/g腦組織,t:4.27,P<0.05].結論 在實驗性甲狀腺功能亢進癥(簡稱甲亢)時,腦紋狀體DA及其代謝產物的閤成及代謝加快,與99Tcm-TRODAT-1結閤的DAT水平則齣現下降趨勢.
목적 연구갑상선격소승고시뇌문상체다파알전운단백(DAT)、다파알(DA)급기대사산물3,4이간분을산(DOPAC)적함량변화.방법 장24지대서분위2조,기중일조채용관위법련속매천급여대서좌갑상선소납(우갑악,용량안대서체질량25 ug100 g,용우생리염수중,체적1ml조성실험성대서갑상선격소증고상태;대조조부채용관위법급여생리염수1 ml;우모형건립전후안수궤수자표법추취좌갑상선소납조급대조조대서각6지,분별취미정맥혈1 ml,채용방사면역법측정TT3급TT4수평.14 d후장상술대조조급좌갑상선소납조대서재각자평균분위2조,일조용우99Tcm-2B-[N,N'일쌍(2-구을기)을탱이알기]갑기-3B-(4-록분기)탁완(TRODAT-1)대서뇌내분포연구,령일조용우고효액상일전화학검측기(HPLC-ECD)측문상체DA급DOPAC함량,관찰상술대서뇌문상체구DAT적분포급DA、DOPAC함량적변화.다조비교채용ANOVA분석,2조간비교채용균수t검험.결과 좌갑상선소납조대서재복용좌갑상선소납후활동명현증가,체질량증장저우정상대조조[(223.90±8.40)여(261.60±14.20)g,t=6.98,P<0.05].갑상선격소수평측정시:좌갑상선소납조급약전후TT3분별위(1.46±0.17)화(2.72±O.29)nmol/L(t=10.51.P<O.0001);TT4분별위(83.52±10.06)화(187.12±36.48)nmol/L(t=7.74,P<0.0001);대조조생리염수주사전후,TT3분별위(I.54±O.09)화(I.71±0.20)mot/L(t=1.68,P>0.05),TT4분별위(98.38±9.77)화(88.38±6.76)mnel/L(t=1.36,P>0.05).99Tcm-TRODAT-I대서뇌내분포(매극뇌조직백분주사제량솔,%ID/g)연구시:좌갑상선소납조문상체위(2.80±O.25)%ID/g,명현저우정상대조조[(3.13±O.14)%ID/g,t=일3.62,P<0.05].경검측좌갑상선소납조뇌문상체DA함량위(12 109.30±629.20),ng/g뇌조직,명현고우대조조[(9012.CO±318.80)ng/g뇌조직,t=10.76,P<O.0001];DOPAC함량위(1079.30+97.00)ng/g뇌조직,역고우대조조[(873.20±67.50)ng/g뇌조직,t:4.27,P<0.05].결론 재실험성갑상선공능항진증(간칭갑항)시,뇌문상체DA급기대사산물적합성급대사가쾌,여99Tcm-TRODAT-1결합적DAT수평칙출현하강추세.
Objective The central dopamine system plays an important role in regulating movementand mood.In our routine clinical practice,we found that patients with hyperthyroidism often show tremor oftheir hands or legs.Meanwhile they also experienced emotional problems such as anxiety and depression.These reminded us that there might be close relationship between thyroid hormone and dopamine system.Based on our previous works,in this study we evaluated the change of striatum dopamine transporter(DAT)by using 99Tcm-2β-[N,N'-bis(2-mereaptoethyl)ethylenediamino]methyl,3β-(4-chlorophenyl)tropane (TRODAT-1) brain biodistribution,and the content of striatum dopamine and its metabolites 3.4-di-hydroxyphenylacetic (DOPAC)by high performace liquid chromatograph-electrochemical detection(HPLC-ECD)in rats with experimental hyperthyroidism.Methods All 24 rats were randomly divided into two groups,thyroxin group were induced by daily thyroxin infusion for 14d to be experimental hyperthyroidism,the others were control group which received saline infusion.The blood samples were randomly taken from thyroxine group and control group,and the concentration of Tr3 and 13"4 were detected by radioimmunoas-say.After 14 d,both experimental (thyroxine group) and control group were further divided into 2 sub-groups.One was for evaluation of the function of striatum DAT by 99Tcm-TRODAT-1 biodistribution study and the other was for HPIJC-ECD measurement of the concentration of dopamine and its metabolites DOPAC.Results Significantly hyperactivity and weight loss[(223.90±8.40)VS(261.60±14.20)g,t=6.98.P<0.05] in thyroxin than control groups were observed.Of the thyroxin group.the concentration of TT3 and TT4 after thyroxin infusion was significantly elevated than that of before thyroxin infusion[(2.72±0.29)V8(1.46±0.17)nmol/L,t=10.51,P<0.0001.and (187.12±36.48) vs (83.52±10.06)nmol/L,t=7.74,P<0.0001].Of the control group,the concentration of TT3,and TT4 after saline infu-sion showed no statistical significance as compared with before saline infusion [(1.71±0.20) vs (1.54±O.09)nmol/L,t=1.68.P>0.05 and(88.38±6.76) vs (98.38±9.77)nmol/L.t=1.36.P>O.05].For 99Tcm-TRODAT-l brain biodistribution(the percent injection dose of per gram of tissue,%ID/g),the striatum of thyroxin group was(2.80±0.25)%ID/g,lower than that of the control group [(3.13±0.14)%ID/g,t=-3.62,P<0.05].The content of striatum DA was(12 109.30±629.20)ng/g brain tissue,higher than that of the control[(9012.00±318.80)ng/g brain tissue,t=10.76.P<0.000l 1],the content of DOPAC Was(1079.3±97.00)ng/g brain tissue,higher than that of the control[(873.20±67.50)ng/g brain tissue,t=4.27.P<0.05].Conclusions Decreased 99Tcm-TRODAT-1 uptake was ob-served in striatum DAT during rats with experimental hyperthyroidism.The possible reason might be due to the increased dopamine binding with DAT.
