CAJ | 학술논문

目的研究核酸内切酶(Dicer)在肝门部胆管癌组织和胆管癌细胞株(QBC939)中的表达及其与临床病理因素和预后的相关性.方法采用免疫组化染色检测40例肝门部胆管癌和10例正常胆管组织中Dicer的表达；Western-blot和RT-PCR检测人胆管癌细胞株QBC939与人胆管上皮细胞株HIBEpic中Dicer的表达量；分析Dicer表达与临床病理因素的相关性.对根治性切除的肝门部胆管癌患者术后总生存期和无病生存期进行单因素和多因素分析.结果 Dicer在肝门部胆管癌组织中的表达明显低于正常胆管组织(P＜0.05),在QBC939中的表达明显低于HIBEpic(P＜0.05),高分化胆管腺癌中Dicer表达明显高于中、低分化腺癌.单因素生存分析结果显示Dicer低表达组的根治性切除术后总生存期和无病生存期均短于高表达组(P＜0.01).多因素分析显示Dicer表达水平为与预后相关的独立危险因素(P＜0.05).结论在肝门部胆管癌和胆管癌细胞株Dicer表达下调；低表达Dicer的胆管癌患者预后更差.Dicer可能为肝门部胆管癌的诊断和判断预后的分子标志物.
목적 연구핵산내절매(Dicer)재간문부담관암조직화담관암세포주(QBC939)중적표체급기여림상병리인소화예후적상관성.방법 채용면역조화염색검측40례간문부담관암화10례정상담관조직중Dicer적표체；Western-blot화RT-PCR검측인담관암세포주QBC939여인담관상피세포주HIBEpic중Dicer적표체량；분석Dicer표체여림상병리인소적상관성.대근치성절제적간문부담관암환자술후총생존기화무병생존기진행단인소화다인소분석.결과 Dicer재간문부담관암조직중적표체명현저우정상담관조직(P＜0.05),재QBC939중적표체명현저우HIBEpic(P＜0.05),고분화담관선암중Dicer표체명현고우중、저분화선암.단인소생존분석결과현시Dicer저표체조적근치성절제술후총생존기화무병생존기균단우고표체조(P＜0.01).다인소분석현시Dicer표체수평위여예후상관적독립위험인소(P＜0.05).결론 재간문부담관암화담관암세포주Dicer표체하조；저표체Dicer적담관암환자예후경차.Dicer가능위간문부담관암적진단화판단예후적분자표지물.
Objective To investigate the relationship between Dicer expression and clinicopathological characteristics and prognosis by detecting the expression of Dicer in hilar cholangiocarcinoma tissues and cells.Methods The expression of Dicer in tissues was detected using immunohistochemistry.Western blotting and RT-PCR were used to investigate Dicer expression in QBC939 and HIBEpic cells.The relationship between Dicer expression and clinicopathological characteristics was analyzed.A Kaplan-Maier analysis was performed to analyze the disease-free survival (DFS) and overall survival (OS) after radical surgical resection of hilar cholangiocarcinoma.Results When compared to control,Dicer was significantly down-regulated in hilar cholangiocarcinoma tissues (P＜0.05) and in QBC939 (P＜0.05).The expression of Dicer was higher in well differentiated adenocarcinoma than poorly and moderately differentiated tumours. Univariate analysis showed low expression of Dicer protein was significantly correlated with short disease-free survival and overall survival of patients with hilar cholangiocarcinoma after radical surgical resection (P＜0.01). Multivariate analysis revealed that the expression of Dicer was the most important factor for predicting prognosis after radical surgical resection of hilar cholangiocarcinoma (P＜0.05).Conclusions Dicer could be used as a prognostic marker for hilar cholangiocarcinoma.