CAJ | 학술논문

目的观察脂多糖(LPS)体外作用于大鼠肝星状细胞(HSC)时肿瘤坏死因子-α(TNF-α)和结缔组织生长因子(CTGF)表达变化,探讨TNF-α对LPS诱导的HSC中CTGF表达的影响.方法用一步密度梯度离心法分离大鼠HSC,体外培养LPS处理HSC,逆转录-聚合酶链反应(RT-PCR)方法检测不同LPS浓度处理的HSC CTGF mRNA表达水平,并用ELISA双抗体夹心法测定TNF-α浓度.结果 TNF-α水平随LPS浓度增加而增加,LPS可上调CTGF mRNA的表达,TNF-α浓度及CTGF mRNA表达水平呈一定的平行关系.结论 LPS可上调HSC中CTGF mRNA表达,该过程可能是通过TNF-α介导的.
목적 관찰지다당(LPS)체외작용우대서간성상세포(HSC)시종류배사인자-α(TNF-α)화결체조직생장인자(CTGF)표체변화,탐토TNF-α대LPS유도적HSC중CTGF표체적영향.방법 용일보밀도제도리심법분리대서HSC,체외배양LPS처리HSC,역전록-취합매련반응(RT-PCR)방법검측불동LPS농도처리적HSC CTGF mRNA표체수평,병용ELISA쌍항체협심법측정TNF-α농도.결과 TNF-α수평수LPS농도증가이증가,LPS가상조CTGF mRNA적표체,TNF-α농도급CTGF mRNA표체수평정일정적평행관계.결론 LPS가상조HSC중CTGF mRNA표체,해과정가능시통과TNF-α개도적.
Objective To explore the effect of lipopolysaccharide(LPS)on the expression of connective tissue growth factor(CTGF)in rat hepatic stellate cells(HSC)in vitro and the effect of tumor necrosis factor-α(TNF-α)on the expression of CTGF in HSC induced by LPS.Methods Rat HSCs were separated by in situ perfusion of pronase and eoUengase,and single-step Nyeodenz density gradient centrifugation.After in vitro cultare and passages.HSCs were treated by E coli-derived LPS.The CTGF mRNA expression was detected by simiquantitative RT-PCR with difierent LPS concentrations.TNF-α was determined by enzyme-linked immunosorbnent assay.Results The level of TNF-α was increased with the increased concentrations of LPS,and LPS could also up-regulate the expression of CTGF mRNA and there existed a parallel relationship.Conclusion LPS can up-regulate the expression of CTGF mRNA of HSCs,and TNF-α might play an important role in the pathological process of the expression of CTGF mRNA.